The ability to regenerate tissues is shared across many metazoan taxa, yet the type and extent to which multiple cellular mechanisms come into play can differ across species. For example, urodele amphibians can completely regenerate all lost tissues, including skeletal muscles after limb amputation. This remarkable ability of urodeles to restore entire limbs has been largely linked to a dedifferentiation-dependent mechanism of regeneration. However, whether cell dedifferentiation is the fundamental factor that triggers a robust regeneration capacity, and whether the loss or inhibition of this process explains the limited regeneration potential in other vertebrates is not known. Here, we studied the cellular mechanisms underlying the repetitive regeneration of myogenic tissues in the electric fish S. macrurus. Our in vivo microinjection studies of high molecular weight cell lineage tracers into single identified adult myogenic cells (muscle or noncontractile muscle-derived electrocytes) revealed no fragmentation or cellularization proximal to the amputation plane. In contrast, ultrastructural and immunolabeling studies verified the presence of myogenic stem cells that express the satellite cell marker Pax7 in mature muscle fibers and electrocytes of S. macrurus. These data provide the first example of Pax-7 positive muscle stem cells localized within a non-contractile electrogenic tissue. Moreover, upon amputation, Pax-7 positive cells underwent a robust replication and were detected exclusively in regions that give rise to myogenic cells and dorsal spinal cord components revealing a regeneration process in S. macrurus that is dependent on the activation of myogenic stem cells for the renewal of both skeletal muscle and the muscle-derived electric organ. These data are consistent with the emergent concept in vertebrate regeneration that different tissues provide a distinct progenitor cell population to the regeneration blastema, and these progenitor cells subsequently restore the original tissue.
Citation: Weber CM, Martindale MQ, Tapscott SJ, Unguez GA (2012) Activation of Pax7-Positive Cells in a Non-Contractile Tissue Contributes to Regeneration of Myogenic Tissues in the Electric Fish S. macrurus. PLoS ONE 7(5): e36819. doi:10.1371/journal.pone.0036819
Editor: Marcello Rota, Brigham & Women’s Hospital - Harvard Medical School, United States of America
Received: August 25, 2011; Accepted: April 15, 2012; Published: May 31, 2012
Copyright: © 2012 Weber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was funded by National Institutes of Health grants S06-GM008136 (GAU), RR16480 (GAU), and U56-CA96286 (GAU and SJT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
A major challenge in the study of mammalian regeneration remains the identification of the limiting factors that constrain the restoration of a tissue with the purpose of modifying these factors to boost the regeneration potential , . Many vertebrate animals repair and remodel their injured or lost tissues primarily through the activation of stem cells and/or the process of dedifferentiation, i.e., a process by which mature cells lose their differentiated phenotype to produce progenitor cells that can reenter the cell cycle. Multiple regeneration mechanisms may come into play in one animal in response to injury or disease. For example, in humans liver tissue regenerates through a dedifferentiation process , whereas skeletal muscle regenerates through the activation of myogenic stem cells ,  suggesting that programs of tissue regeneration might be broadly conserved but their expression is regulated by tissue-specific constraints.
Although one mechanism might be favored under certain conditions, it is not possible to exclude some contribution from an alternative mode of regeneration. Nevertheless, the idea that a greater potential for regeneration is associated with a corresponding potential for cell dedifferentiation remains broadly accepted , , . Support for this idea is based largely on the vast body of evidence from experiments in urodele amphibians such as the newt and axolotls, which are regarded as the champions of regeneration among vertebrates. Urodeles are able to regenerate limbs, tail, jaws, ocular tissues like retina and lens, and some portions of the heart. Studies have suggested that their ability to regenerate these tissues depends largely on dedifferentiation of cells at the site of injury to reenter the cell cycle , . In contrast, there is less evidence for the existence of adult stem cell populations and their contribution to restored tissues in urodeles , .
Some studies using urodele amphibians have indicated that regeneration does not proceed in the absence of cell dedifferentiation , , , . Whether cell dedifferentiation is the fundamental factor that triggers a robust regeneration capacity, and whether the loss or inhibition of this process explains the limited regeneration potential in other vertebrates is not known. In order to test this idea by studying different species, it is important to first recognize that cell dedifferentiation involves different (and likely, independent) cellular events . The first involves the reentry of myonuclei into the cell cycle suggesting that the postmitotic arrest of nuclei in mature muscle fibers is reversible in urodeles . The second type of dedifferentiation involves the reversal of multinucleated muscle fibers to mononucleated cells (aka, cellularization). The third event involves the loss of gene expression markers of the differentiated state such as sarcomeric proteins. Each of these processes has been demonstrated to occur in the dedifferentiation of skeletal muscle fibers after limb amputation in urodeles. However, the extent to which each of these events occurs in response to injury among other vertebrates has not been well studied. Investigation of the regenerative processes in vertebrates other than urodeles with extensive regenerative capacities is essential for the identification of the limiting factors that constrain the restoration of tissues in some species and development of strategies to improve their regeneration potential.
The South American gymnotiform electric fishes are unique vertebrate teleosts in that they possess a muscle-derived electric organ (EO) that is specialized for the production of an electric field outside the body  and they also possess a robust capacity to regenerate lost tissues. Of the seventy known species of gymnotiforms, all can regenerate their tails following amputation. Specifically, they can replace lost spinal cord, skin, electroreceptors, skeleton, blood vessels, skeletal muscle and the muscle-derived electric organ , , , , , , , . Tail regeneration in all gymnotiforms begins by epidermal cells covering the wound followed by formation of a blastema of apparently undifferentiated cells appearing as a small swelling at the end of the tail that gives rise to all newly formed adult tissues , , , a process widespread in the animal kingdom, appearing in both invertebrate and vertebrate organisms , . Moreover, some gymnotiforms can replace all tissues lost after repeated tail amputations, suggesting an inexhaustible regeneration capacity in the adult  and representing the ideal experimental system for investigation of regeneration in teleosts.
Tail regeneration in gymnotiforms has been most studied in the yellow stripe knife fish S. macrurus , , . As in all gymnotiforms, S. macrurus responds to tail amputation by wound healing, blastema formation, and blastema elongation by cell proliferation. At the site of amputation at the end of the ventral fin, electrocytes predominate, but also present are skeletal muscle fibers found peripherally beneath the skin and vertebrae protecting the central artery and motoneurons that innervate electrocytes and muscle fibers (Figure 1). Cell differentiation during regeneration proceeds with slow-twitch fibers appearing under the skin followed by differentiation of fast-twitch fibers medial to the slow fibers . A subpopulation of the most centrally-located fast-twitch fibers then fuses and subsequently downregulates many muscle genes to give rise to cells of the electric organ, i.e., electrocytes, all while the spinal cord, vertebrae and blood vessel are differentiating within the center of the tail , . Within three weeks of tail amputation, a new tail develops complete with peripheral slow and fast muscle fibers and a centrally located electric organ surrounding the vertebrae, spinal cord and central blood vessel .
Figure 1. Schematic illustration of S. macrurus and the amputation protocol used in the present study.
Tails were amputated at the end of the ventral fin (scissors). At the site of amputation, vertebrae, central artery, motor neurons, electrocytes and muscle fibers are present. The spinal cord contains two distinct populations of motor neurons: electromotoneurons that innervate electrocytes and somatomotoneurons that innervate muscle fibers. Muscle fibers are located peripherally and surround a central core of electrocytes. Electrocytes are up to 3 mm in length with a cross sectional area up to 30 times that of the adjacent muscle fibers. As shown in the cross sectional view at the bottom, muscle fibers and electrocytes are separated into distinct compartments. In this study, we analyzed the most-distal segment (50 mm) of the tail stump after tail amputation to investigate effects of amputation on tissues immediately adjacent to the injury site.doi:10.1371/journal.pone.0036819.g001
A previous study looking at the labeling patterns of cell replication markers during tail regeneration in S. macrurus suggested that the source of the regeneration blastema consisted of local reserve cells that became activated to contribute to the restoration of all lost tissues . Specifically, the authors concluded that myogenic satellite cells contributed to the blastema and the regeneration of skeletal muscle and the myogenically derived EO reviving the argument for determining the extent to which stem cell activation and dedifferentiation are conserved across different vertebrate species. Here we tested the hypothesis that myogenic stem cells contribute to blastema formation during adult tail regeneration in S. macrurus. We detected myogenic cells associated with adult skeletal muscle and electric organ that express the mammalian satellite cell marker Pax7 . After tail amputation, these Pax7-positive satellite cells proliferated and populated blastema regions that exclusively regenerate muscle fibers and electrocytes. Accompanying in vivo microinjection studies using high molecular weight lineage tracers showed that multinucleate muscle fibers and electrocytes proximal to the injury site do not undergo fragmentation (cellularization) and do not participate in the formation of regenerated myogenic cells. Our results reveal a regeneration process in S. macrurus that is dependent on the activation of myogenic stem cells for the renewal of both skeletal muscle and the muscle-derived electric organ. These data also suggest that muscle dedifferentiation is not required for a robust regenerative capacity in the myogenic lineage of the adult teleost S. macrurus.
Materials and Methods
Animal Surgeries and Tissue Preparation
S. macrurus is a fresh-water species of knife fish native to South America and was obtained commercially from Segrest Farms (Gibsonton, FL). Adult fish, 20–35 cm in length, were housed individually in 15 to 20-gallon aerated aquaria maintained at 25–28°C and fed three times weekly. Fish were anesthetized in 2-phenoxyethanol (1:1,500 in tank water) and a tail segment distal to the ventral fin was amputated with a sharp blade as described previously , , . In one group the most-distal segment (50 mm) of the tail stump along with the blastema was harvested 7 (n = 4) and 14 (n = 3) days after initial tail amputation. In a second group, only the regeneration blastema was removed 1 (n = 5) or 2 (n = 5) weeks after initial tail amputation. In a third group, only the distal-most section of ventral muscle (2–3 cm) was amputated. In this procedure, electric organ, central blood vessel and spinal cord were left intact and regeneration studied until full restoration of muscle was complete. Immediately following each amputation surgery, fish were treated with a topical antibiotic (Nystatin and Triamcinolone Acetonide Ointment USP), returned to their tanks, Stress Coat® (Aquarium Pharmaceuticals, Inc., Chalfont, PA) was added to the aquarium tank as an additional anti-infection agent, and fish were monitored until they recovered fully from anesthesia. Control fish were overdosed in 2-phenoxyethanol (1:500 in tank water) and the liver, brain, spinal cord, ventral skeletal muscle and EO were excised under a dissecting microscope. All procedures used in this study followed the American Physiological Society Animal Care Guidelines and were approved by the Animal Use Committee at New Mexico State University. Excised tail segments were 1) immediately immersed in RNAlater™ (Ambion, Austin, TX) and stored at −80°C until RNA extraction, 2) frozen directly in liquid nitrogen and stored at −80°C until protein extraction for Western blots, or 3) frozen on cork with Tissue Freezing Medium™ (TBS™, Durham, NC) in isopentane chilled in liquid nitrogen and used for immunohistochemical analysis.
RNA Isolation and Reverse-transcription and Polymerase Chain Reaction (RT-PCR)
Total cellular RNA was isolated from skeletal muscle, EO, spinal cord, brain, and liver of adult control fish using a protocol adapted from the guanidinium isothiocyanate method used in previous studies with S. macrurus myogenic tissues –. To isolate total RNA from distal-most (50 mm) tail stumps and regeneration blastema (1- and 2-week), we used the RNeasy kit (Qiagen, Valencia, CA). To remove residual DNA, total RNA isolated from each tissue sample was treated with DNase I, Amplification Grade (Ambion, Austin, TX) and analyzed by spectrophotometry (OD260/OD280). On average, total RNA isolations yielded 1–3% of starting material from each of the different tissues. All RNA samples were stored at −80°C.
Cloning and Isolation of S. macrurus Pax7
Heterologous degenerate oligonucleotide primers were designed for Pax7 based on respective GenBank vertebrate sequences using Dialign and CODEHOP. Pax7 primers corresponding to the PAPGQNYPRT (sense primer) and GKKDDDDDC (antisense primer) domains were designed using alignments from protein sequences of Danio rerio (GenBank: NP_571400), Salmo salar (GenBank: CAF02090.1), Tetraodon nigroviridis (GenBank: CAG07040.1), and two sequences from Salvelinus alpinus (GenBank: CAG25716.1; CAG25714.1). To clone partial Pax7 cDNAs from S. macrurus, we carried out reverse transcription (RT) and PCR separately. The RT reaction from 250–300 ng total RNA was performed for 50 min at 42°C using the SuperScript™ First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA). PCR amplification (1 µg of 1-week regeneration blastema cDNA) was carried out using PCR with annealing temperature 5°C below the calculated Tm for 35–40 cycles with Platinum® Taq DNA Polymerase (Invitrogen).
RT-PCR products for Pax7 were PCR purified by gel extraction (Qiagen, Valencia, CA), subcloned into the Topo TA2.1 Plasmid (Invitrogen) or pGEMTeasy (Promega, Madison, WI) and transformed into Mach1 cells (Invitrogen) or JM109 cells (Promega). Plasmids from 10–20 cDNA clones of each transcript were isolated using the QIAprep® Spin Miniprep Kit (Qiagen), sequenced in both directions using a Li-Cor 4200 L Global IR2 DNA Sequencer or an Applied Biosystems automated DNA sequencer (Model 3100), and analyzed using the Vector NTI Suite 8.0 software (InforMax, Inc., Bethesda, MD). Upon verification of the cloned sequences obtained from 1-wk cDNA regeneration blastema, nondegenerate primers for the Pax7 transcript specific to S. macrurus were generated from the on-line Primer3 software program (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi).
Molecular Cloning of Full-length Pax7 from S. macrurus
RACE first-strand synthesis from total RNA (1 µg) of 1-week regeneration blastema was performed using the BD SMART™ RACE cDNA Amplification Kit (BD Biosciences). Pax7-specific primers used for 5′-RACE were 5′-GCACACTCCGTCCTTCAGCAGCTTG-3′ and 5′ - GCTGGAATGGCTACTTTACCG 3′, and for 3′-RACE were 5′-GAGACGGGTTCGATTCGTCCTGGAG–3′ and 5′– TAGCCAGGTAGTCCACAGCAC –3′. Purified products from Pax7 RACE experiments were subcloned into the pCR® 2.1-TOPO® vector (Invitrogen), transformed into One Shot® MACH1™ T1 Phage-Resistant Chemically Competent E. coli cells (Invitrogen), isolated with the QIAprep® Spin Miniprep Kit (Qiagen), and sequenced in both directions using either a Li-Cor-4200 L Global IR2 DNA or an Applied Biosystems automated DNA sequencer (Model 3100). Chromatogram traces were analyzed using the Vector NTI Suite 8.0 software (InforMax Inc.). Searches were performed using the Basic Local Alignment Search Tool (BLAST) network service from the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov). Multiple sequence alignments were generated with ClustalW from the European Bioinformatics Institute (http://www.ebi.ac.uk/clustalw). The novel sequence described here and a transcript variant were submitted to NCBI and assigned accession numbers EU624121.2 and EU624121.1 for S. macrurus Pax7.
Fresh-frozen tissue samples were pulverized with a mortar and pestle in liquid nitrogen and total protein lysates were prepared using lysis buffer (1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 10 mM beta-mercaptoethanol, 10 µg/mL PMSF, 5 µg/mL aprotinin, 0.1 mg/mL benzamidine, 1 µg/mL pepstatin A, 1 µg/mL leupeptin, 100 mM sodium orthovanadate, 15 µL/mL Triton X-100®, in Phosphate Buffered Saline (PBS) pH 7.4) on ice for 45 min and homogenized by two 15-sec pulses. Lysates were centrifuged at 14,000 g for 10 min at 4°C and supernatants were collected. Protein concentration was determined using the Bradford assay (Bio-Rad, Hercules, CA).
Proteins (20 µg/lane) were separated by SDS-PAGE alongside Kaleidoscope pre-stained standards on 4–15% Tris-HCl gradient gels (Bio-Rad; 100 V, 120 min) and transferred onto PVDF membranes using a Transblot SD semi-dry electrophoretic transfer cell (Bio-Rad) following manufacturer’s instructions. Membranes were washed twice with TBS-T [0.1% Tween-20 in Tris buffered saline (TBS)], blocked in blocking buffer (3% non-fat dry milk in TBS-T) for 60 min at RT, and incubated with anti-Pax7 antibody (1:50; Developmental Hybridoma Bank, Iowa City, IA) diluted in 5% BSA in TBS-T overnight at 4°C. After PBST washes, membranes were incubated with an HRP-conjugated secondary antibody for 1 hr. The BioRAD amplification module was used according to manufacturer’s instructions. Colorimetric detection via BioRAD’s Opti-4CN™ Substrate Kit was performed according to manufacturer’s instructions to visualize antigen-antibody complexes.
In vitro Translation of S. macrururs Pax7 cDNA
To further verify antibody specificity to S. macrurus Pax7 protein, a 1,435 bp Pax7 fragment from muscle was ligated into the pGEMTeasy vector, (Promega), and subcloned. Following sequence verification, the insert from muscle cDNA was translated in vitro using the TNT® T7 Coupled Reticulocyte Lysate System (Promega) following manufacturer’s instructions. Translation product was then separated by SDS PAGE (product diluted 4× in 2× SDS load buffer & 20 µL, 25 µL, and 30 µL were loaded), transferred to PVDF, and probed with the Pax7 antibody as described above.
Normal adult tails (n = 2) were examined under a transmission microscope (TEM). After surgical removal, blastemas and tails were fixed in 4% paraformaldehyde/2.5% gluteraldehyde overnight, transferred to sodium phosphate buffer (pH = 7.2), post-fixed in osmium (2% osmium in sodium phosphate buffer) for 1 hour, dehydrated in an alcohol series and propylene oxide, and embedded in Spurrs’ plastic resin (Polysciences, Warrington, PA). Ultrathin (90-nm) tissue cross sections were cut with a diamond knife, stained with uranyl acetate and lead citrate, and examined with the Hitachi HU11-E TEM (Cell Research Institute of the University of Texas at Austin).
Serial transverse and longitudinal cryosections (14–30 µm thick) were mounted on glass slides (Superfrost Plus, Fisher, Pittsburgh, PA) and air-dried at room temperature. Sections were surrounded with a hydrophobic barrier by using an ImmEdge pen (Vector Laboratories, Ingold, CA) to retain incubating solutions on the tissue. Sections were post-fixed on slides by immersion in 4% buffered formaldehyde (pH 7.4, 20 min, 37°C) and rinsed twice in 0.1 M PBS (pH 7.4). Slides were immersed in permeabilization solution (0.5% Triton® X-100 in PBS for 30 min.) and subsequently incubated in 1% blocking reagent (TSA kit, Invitrogen) for 1 hr at room temperature. Mouse monoclonal antibodies against Pax7 (1:20), sarcomeric myosin heavy chain isoforms (MF20, 1:100), and laminin (1:100) were diluted in 1% blocking solution and applied to separate slides for 15–20 h at room temperature. Antibodies against Pax7 and MF20 were obtained from the Developmental Hybridoma Bank (Iowa City, IA) and the anti-laminin antibody was obtained from Sigma (St. Louis, MO). Tissue sections were incubated in 1% blocking solution without primary antibodies to assess nonspecific labeling by secondary antibodies. Sections were rinsed in PBS and incubated in horseradish peroxidase-conjugated goat anti-mouse IgG (TSA kit, diluted 1:100 in 1% blocking solution) for 1 h. Tissue sections were rinsed in PBS, and incubated in Alexa Fluor 488 or 546 tyramide working solution (TSA kit, 10 min., diluted 1:1000 in 0.0015% H2O2). Sections were rinsed in PBS, counterstained with propidium iodide (Sigma; 1:1000, 1 min) or SYTOX Green nucleic acid stain (Invitrogen, 1:1000, 1 min), rinsed in deionized water, and cover-slipped with Vectashield Hard Set mounting media (Vector) or Gelmount (Biomeda, Foster City, CA).
Fish were given intraperitoneal injections of BrdU (Sigma) at a dose of 100 mg/kg. Fish were given two BrdU injections: immediately after tail amputation and 24 h after tail amputation. In these fish, the distal-most 50 mm portion of the tail stump was removed 7 or 14 days post amputation. For each group, non-amputated control fish were injected (n = 2) to determine the level of cell replication in the absence of tail amputation. The fates of BrdU-labeled cells in regeneration blastema were assessed 1 (n = 5) and 2 (n = 5) weeks after tail amputation. Serial transverse and longitudinal cryosections (20–30 µm thick) were mounted on glass slides, and air-dried at room temperature. Slides were immersed in 0.1 M PBS for 5 min followed by incubation in HCl solution (1.5 mL HCl/23.5 mL ddH2O) for 30 min. at 37°C. Slides were rinsed in PBS, incubated in.1 M sodium borate (pH = 7–8) for 10 min at room temperature, and then immersed in 1% blocking solution (Invitrogen) for 30 min prior to incubation in mouse anti-BrdU antibody (BD biosciences, San Jose, CA; 1:20) for 15–20 h at room temperature. Slides were rinsed in PBS and incubated in fluorescein-conjugated secondary antibody (Alexa Fluor 488 or 546; dilution, 1:200; Molecular Probes, Eugene, OR) for 1 hr. Sections were again rinsed in PBS, counterstained in propidium iodide or SYTOX, rinsed in deionized water, and cover-slipped with Vectashield Hard Set mounting media (Vector) or Gelmount (Biomeda).
Intracellular Dye Injections
To test the incidence of cell dedifferentiation during regeneration in S. macrurus, fluorescent lineage tracer lysinated tetramethylrhodamine (Fluororuby; 10,000 MW; Molecular Probes, Inc.) was pressure-injected with glass micropipettes and a picospritzer into individual electrocytes or muscle fibers (n = 10–15/fish) of a total of 22 fish under a Zeiss Axiovert 100 M fluorescent microscope. A flap of skin was reflected to allow visualization and access to individual cell types. Fluororuby exhibits low toxicity and is retained in cells for periods of up to 3–4 weeks with little incidence of diffusion ; . Tails were transected immediately after muscle fibers and electrocytes were microinjected with the dye and cell filling with the dye verified under a fluorescent scope. At 1-, 2-, 3-, 4-, 5- and 6-days post-amputation, proximal stumps were processed for cryosectioning and immunolabeling with Pax7 and MF20 antibodies using methods described above.
Images of immunolabeled tissue sections were visualized and captured on a BioRad 1024 confocal microscope (Bio-Rad Laboratories, Richmond, CA), controlled by OpenLab imaging software (Improvision, Lexington, MA). To quantify Pax7-positive cells in control versus regeneration, images from separate samples of proximal tissue at day 7 (n = 4), control (n = 3) and day 7 blastema (n = 3) were sampled using ImageJ. In each image, three different 100 µm2 areas with highest Pax7-positive density were converted to 8-bit and the threshold was modified to reflect positively labeled cells. For proximal and control samples, cells were manually counted using the cell counter plugin. Day 7 blastema was counted using the analyze particle function and the results were displayed to validate correct counts. Manual counting produced the same numbers as produced by the analyze particle plugin. Counts for each image were averaged and the average value from the set of images for each experimental condition were graphed with error bars representing the standard deviation. To quantify colocalization of Pax7 and BrdU, day 7, day 14, and control (n = 3) images were used. For each image, red and green channels were converted to 8-bit and the JACoP plugin  was used to calculate Pearson’s coefficient.
S. macrurus Pax7 Protein is Highly Conserved
PCR-coupled RACE generated two Pax7 cDNA sequences from S. macrurus skeletal muscle and electric organ tissues: a 2,187 bp cDNA (GenBank accession number EU624121.1) and a 1,809 bp cDNA (GenBank accession number EU624122.1). The longest cDNA contains a 507 amino acid open reading frame that is flanked by a 216 bp 5′ UTR and a 447 bp 3′ UTR. Multiple sequence alignments of the predicted S. macrurus Pax7 protein with mouse (accession number NP_035169.1; Figure 2A), and other teleosts including zebrafish (D. rerio; accession number NP_571400), Atlantic salmon (S. salar; accession number CAF02090.1), and arctic char (S. alpinus; accession number CAG25716.1) (Figure 2B) revealed a high degree of conservation among these Pax7 amino acid sequences. The similarity between Pax7 of S. macrurus, mouse, and other piscine species ranged from 87% (mouse) to 96% identity (D. rerio) (Figure 2). Vertebrate Pax7 contains distinct protein regions that are required for transcriptional activation of its target genes, i.e., the paired box domain, the octapeptide, and the homeodomain (Figure 2A; 37; 38). Within the paired box domain, S. macrurus Pax7 showed a 93% homology compared to the mouse protein (Figure 2A), and 98–100% homology to that of other teleosts (Figure 2B). The octapeptide was 100% similar to both mouse and teleost regions (Figure 2), and the homeodomain region of S. macrurus Pax7 differed only at one of sixty amino acids (98%) with the other vertebrate Pax7 sequences. In sum, S. macrurus Pax7 protein exhibits a high degree of sequence conservation in the specific amino acids that are important for its transcriptional activation. The shorter Pax7 variant contains a 300 amino acid open reading frame that is flanked by a 216 bp 5′ UTR and a 689 bp 3′ UTR (data not shown). The sequences of the three functional protein domains, the paired box domain (100%), the octapeptide (100%), and the homeodomain (78%) were highly conserved between the two Pax7 variants in S. macrurus. Compared to the longer variant, the shorter Pax7 variant was truncated downstream of the homeodomain resulting in a shorter putative protein by 207 amino acids.
Figure 2. S. macrurus Pax7 protein is highly conserved.
Sequence comparison of the deduced protein encoded by S. macrurus Pax7 (GenBank accession number ACC86106) with comparable sequences from mouse (A) and other piscine species (B). Protein sequences from mouse (M. musculus, GenBank accession number NP_035169.1), zebrafish (D. rerio; accession number NP_571400), Atlantic salmon (S. salar; accession number CAF02090.1), and arctic char (S. alpinus; accession number CAG25716.1) were used. The boxed areas are labeled to indicate the highly conserved regions of members of the Pax family: the paired domain, octapeptide, and homeodomain. Asterisks represent amino acid similarities across sequences.doi:10.1371/journal.pone.0036819.g002
Pax7 Expression in Adult Tissues
We used RT-PCR and immunolabeling to compare the tissue-specific patterns of Pax7 mRNA and protein expression, respectively, in adult S. macrurus tissues. Pax7 transcript was detected in mature skeletal muscle, electric organ, brain, and spinal cord tissues, but not in liver (Figure 3A). This tissue expression pattern in S. macrurus is consistent with that reported in other vertebrates , . In vitro translation of a partial 1,435-bp Pax7 cDNA transcript generated protein products that immunoreacted with the mouse monoclonal Pax7 antibody obtained from the Developmental Hybridoma Bank (Figure 3B). A band corresponding to the predicted S. macrurus Pax7 protein size of ~55 kDa was strongly labelled and two smaller bands of sizes between 44–55 kDa were also detected (Figure 3B). All bands detected correspond to sizes that are within the range in Pax7 protein sizes found in mouse (~55 kDa; accession number P47239), and other teleosts including zebrafish (~56 kDa; accession number NP_571400.1), arctic char (~55 kDa; accession number Q5ZNB2), and Atlantic salmon (~56 kDa; accession number Q683Y9). Western blots using S. macrurus control skeletal muscle and electric organ tissues revealed a single band of approximately 55 kDa. These data provide support for the specificity of the mammalian Pax7 antibody against S. macrurus Pax7 protein. It was not determined whether the multiple in vitro translation products were due to internal translation initiation of the cDNA. However, proteins of approximately 54, 53, 49, and 43 kD could be explained by leaky initiation of translation at the first four internal in-frame ATG residues of the S. macrurus Pax7 mRNA. On tissue sections, Pax7 co-localized with laminin immunolabeling in both muscle fibers and electrocytes (Figure 3C). Ultrastructural analysis using electron micrographs of longitudinal and cross sections from muscle and electric organ of adult tails corroborated the location of mononuclear cells beneath the basement membrane of muscle cells and electrocytes (Figure 3D). In control tails, the incidence of Pax7-positive cells that incorporated BrdU was relatively low (Figure 3E). By virtue of their Pax7 expression, morphology, and location, these cells associated with muscle fibers and electrocytes can be considered to be homologs of satellite cells observed in mammals , , salamanders , frogs , and other teleosts  and birds .
Figure 3. Pax7 labels myogenic satellite cells in adult skeletal muscle and electric organ of S. macrurus.
(A) Pax7 RT-PCR shows expression in skeletal muscle (SM), electric organ (EO), brain (B), spinal cord (SC), but not liver (L). Negative controls for each sample omitted reverse transcriptase (nrt). (B) Pax7 antibody detects in vitro translation products from partial Pax7 cDNA sequence (1,435 bp) and expected band (~55 kDa) in homogenates (20 µg/lane) from control muscle (M) and electric organ (EO). (C) Pax7-positive cells (red) co-label with laminin (green) in tissue cryosections (20-µm thick). (D) Electron micrograph cross-sections of adult intact tail show satellite cells (SC) unlike other nuclei (N) are found between the plasma membrane (arrow) and basal lamina in both electrocytes and muscle fibers. (E) Few Pax7 cells (green) colabel with BrdU (red) in control longitudinal sections (20-µm thick) (2 Arrow points to co-localization of Pax7 and BrdU. Abbreviations: EC, electrocyte; mm, muscle fiber; epi, epidermis. Scale bars: 50 µm.doi:10.1371/journal.pone.0036819.g003
Spatial distribution of Pax7-positive cells in regeneration blastemas.
Characterization of the tail regeneration process after tail amputation in S. macrurus has been described in previous studies , , . Briefly, when the tail is amputated at the end of the ventral fin, epidermal cells at the wound margin rapidly proliferated and covered the wound within 24 hr. After one week, blastemas reached an average length of 4 mm (range: 2.2–4.8 mm; SD: ±1.1) and were composed primarily of undifferentiated mesenchymal cells. During the second week, blastemas grew to an average length of 7 mm and contained spinal neurons, muscle fibers and electrocytes at different stages of differentiation. Muscle fibers formed adjacent to the epithelium with some of the larger muscle cells farthest from the epithelium fusing and converting into electrocytes – data that supports previous observations in S. macrurus tail regeneration , , .
To determine whether Pax7-positive cells are associated with the restoration of myogenic cells in the blastema, we studied the expression and distribution of Pax7 at different stages of regeneration. Within the first week after amputation, most Pax7-positive cells were detected peripherally underneath the epithelium of the blastema (arrowheads, Figure 4A). This distribution was also true for 2-week blastemas, i.e., Pax7-positive cells were abundant and highly concentrated beneath the epithelium (Figure 4B). Blastema regions under the epithelium correspond to those that give rise to myogenic cells as evident by co-labeling with antibodies against muscle markers desmin (Figure 4C) and sarcomeric myosin heavy chain (Figure 5B). The co-localization of Pax7-positive cells within developing muscle fibers and electrocytes (Figures 4C and 5A-B) clearly demonstrate the contribution of Pax7-positive cells to the formation of the same myogenic tissues they are associated with in adult intact tails. Outside of these myogenic zones in the blastema, Pax7 immunolabeling was only detected in cells along the entire antero-posterior axis of the regenerating spinal cord (Figure 5A, C). The occurrence of Pax7-positive cells exclusively in the dorsal zones of the spinal cord in blastema and adult tails of S. macrurus is consistent with expression patterns reported in developing and regenerating spinal cords in other vertebrates , , –. In sum, our data support a mechanism of regeneration that is mediated primarily through a population of Pax7-positive stem cells that give rise to dorsal spinal cord, muscle fibers and electrocytes.
Figure 4. Spatial distribution of Pax7-positive cells in regeneration blastema.
Confocal images of longitudinal cryosections (20-µm thick) from 7-day (A) and 14-day (B) blastemas immunolabeled with anti-Pax7 (A, B, C), BrdU (B) and desmin (C) antibodies. Dashed line in A denotes the site of amputation with the newly regenerated blastema to the right of the amputation site. Arrows point to electrocytes damaged by amputation. Arrowheads point to Pax7-positive cells adjacent to the epithelium (Ep). Pax7-positive cells are red in A and C, and green in B. Images in C represent the region enclosed in the dotted box in B from a serial cryosection co-labelled with anti-desmin and Pax7 antibodies. Scale bars: A, 400 µm; B, 150 µm; C, 50 µm.doi:10.1371/journal.pone.0036819.g004
Figure 5. Pax7-positive cells are localized in blastema regions that give rise to muscle, electric organ and dorsal spinal cord.
Serial cross-sections taken from distal half of 14-day blastemas (A and B) immunolabeled with antibodies against Pax7 (A, green) and MF20 (B, green). MF20 labels all mammalian myosin heavy chain (MyHC) isoforms present in all muscle fibers and developing electrocytes that arise from the fusion of muscle fibers. Immunolabeling by Pax7 and MF20 was detected in peripheral regions of the blastema underneath the epithelium. Pax7 immunolabel was also detected in cells within the dorsal spinal cord of regenerating blastema (A, arrowhead). C, Pax7 immunolabeling was detected along the entire regenerating dorsal spinal cord in 14-day blastemas as shown in this longitudinal section. Abbreviations: ECs, electrocytes; mm, muscle fibers; SC, spinal cord. Scale bars: A, 300 µm; B, 300 µm; C, 150 µm.doi:10.1371/journal.pone.0036819.g005
Pax7 expression increases in tissues proximal to the amputation site.
Changes in Pax7 expression were determined at different time periods after amputation in the tail region proximal to the amputation site, a tail segment that included both injured and intact cells (Figure 6). Electrocytes injured by tail transection were easily distinguished from intact electrocytes based on their staining with hematoxylin and eosin (Figure 6A–B). Similar distinctions among muscle fibers before and after injury were not made due to their much smaller size and number. Injured electrocytes atrophied into long thin strips that extended into the regeneration blastema (Figure 6A–B). At seven days after amputation, these thin strips of damaged electrocytes contained Pax7-positive cells (Figure 6C–D). BrdU incorporation studies showed higher Pearson’s correlation r values for colocalization of Pax7 and BrdU (Figure 6E) in tails from both 7 and 14 days after amputation compared to tails from control uncut fish (Figure 6F), providing further support that they are the product of a recent cell division. The longitudinal cryosections shown in Figure 7 are representative of the density of Pax7-positive cells proximal to the amputation site observed in all fish studied. We observed an increase in the density of Pax7-positive cells associated with electrocytes and muscle fibers in the proximal stump seven days after tail amputation (Figure 7A) that was >8× higher than that in control uncut tails (Figure 7B). The increased density of Pax7-positive cells in blastema was >100× higher than found in control tissues (Figure 7B). By day 14 post-amputation, most Pax7-positive cells occupied the extracellular space (Figure 7C–D) a distribution not observed in control intact tails (Figure 3C).
Figure 6. Degeneration of electrocytes injured by amputation.
Portions of longitudinal cryosections (14–30 µm thick) from 7-day (A) and 10-day (B) blastemas stained with hematoxyline and eosin. (C) 7-day blastema co-labeled with anti-Pax7 (red) and anti-laminin (green) antibodies. (D) 7-day blastema co-labeled with anti-Pax7 (red) and anti-BrdU (green) antibodies. Dashed line in A, B and D show the sites of tail amputation. Arrows in A, B, C and D point to electrocytes that were damaged by the amputation. E, Enlargement of the region within the dashed area in D to more clearly visualize the colocalization of Pax7 (red) and BrdU (green) as yellow. F, Correlation of Pax7 and BrdU immunolabeling in cells from control tails (Con), and tails after amputation at 7 (7 d) and 14 (14 d) days. Abbreviations: EC, electrocyte; Ep, epithelium; mm, muscle fiber. Scale bars: A, 2 mm; B, 5 mm; C, 500 µm; D, 200 µm. F, Range of Pearson’s correlation values for Pax7/BrdU co-localization for images from 3 different tails taken from control fish, and 7-day and 14-day amputated tails.doi:10.1371/journal.pone.0036819.g006
Figure 7. Pax7 expression increases proximal to the amputation site. A
, Black and white image of a portion of a longitudinal cryosection (20-µm thick) of a 7-day tail immunolabeled with the antibody against Pax7. Proximal stump is the region below to the dashed line and regeneration blastema is the region above dashed line. B, The density of Pax7-positive cells is increased in the proximal stump (>8×) (stump) and regeneration blastema (>100×) (blast) compared to control, based on images from 4 different tails taken from control fish, and 7-day amputated tails. C and D, Portions of longitudinal sections (20-µm thick) of 14-day amputated tails immunolabeled with antibodies against Pax7 (green) and BrdU (red). Dashed lines denote the site of amputation with the proximal stump to the left. Arrows point to cells that were co-labeled with Pax7 and BrdU. Abbreviations: EC, electrocyte; Ep, epithelium. Scale bars: A, 250 µm; B, 100 µm.doi:10.1371/journal.pone.0036819.g007
Injury-dependent responses of muscle and Pax7 expression.
To further investigate the regenerative potential of skeletal muscle Pax7-positive cells in S. macrurus, a section of ventral muscle was amputated. In this procedure, electric organ, central blood vessel and spinal cord were left intact. Pax7 immunolabeling and BrdU incorporation studies were done in the same way as with studies after tail amputation described above. The response to removal of ventral muscle was similar to the process observed after tail amputation, i.e., immediate formation of a wound followed by the formation of a blastema and its growth to give rise to new ventral muscle (Figure 8). Full restoration of all ventral muscle tissue removed was generally completed 20 to 22 weeks after its removal, that is, 15 to 18 weeks longer than when a similar length of muscle was removed after complete tail amputation. Pax7-positive cells were detected within muscle fibers adjacent to the amputation site. However, despite the lack of quantitative assessment, the apparent number of Pax7-positive cells observed in response to tail amputation appeared higher than that observed in response to ventral muscle amputation. This difference is visibly evident in Figures 7 and 8 wherein the incidence of Pax7-positive cells within a 200 µm segment of tissue in tails after amputation (Figure 7) is higher compared to that within a 200 µm segment of tissue after ventral muscle amputation (Figure 8). BrdU incorporation was observed but mainly within the fin and not in muscle fibers adjacent to the injury sites (Figure 8). These data could be explained by an injury response that involves a differential potential in the activation of Pax7-positive cells under different injury scenarios in S. macrurus – a hypothesis that will be tested in future studies.
Figure 8. Delayed regeneration of ventral muscle associated with fewer Pax7-positive cells.
An adult S. macrurus and delineation of its ventral and dorsal muscle groups. Four images show the regeneration of the most distal portion (2 to 3 cm) of the ventral muscle over a 120-day period after its ampuation. Blue arrow points to the distal ventral muscle region removed including portions of the ventral fin. Serial longitudinal cryosections of a tail 10 days after ventral muscle excision were immunolabeled with anti-Pax7 (red) antibody to show the incidence of Pax7 immunolabeling at two different depths of the tail region adjacent to the muscle excision area. Dotted lines on the top two Pax7 images represent site of ventral muscle excision. The bottom panel shows longitidunal section from the same animal immunolabeled with anti-BrdU (red) and MyHC (MF20; green) antibodies. A dotted line has been added to the bottom image (BrdU/MyHC) to clearly discern the boundary between skeletal muscle and fin adjacent to the site of excision. MyHC is not expressed in the fin. Scale bar for Pax7/BrdU immunolabeled images: 200 µm.doi:10.1371/journal.pone.0036819.g008
No Evidence for Myogenic Cell Fragmentation after Tail Amputation
The occurrence of cell dedifferentiation has been a major focus in urodele limb regeneration studies. Morphological and cell tracing experiments, for instance, have demonstrated that mature skeletal muscle fibers can fragment (or cellularize) into smaller nuclei-containing units, proliferate, and contribute as a source of new cells that make up the blastema –. In this study, when we stained longitudinal tissue sections with hematoxylin and eosin (Figure 6A–B) or immunolabeled them with anti-laminin (Figure 9A–B), we did not observe changes in cell morphology of muscle fibers and electrocytes proximal to the transection site that resembled cell fragmentation. Similarly, our BrdU incorporation studies did not reveal labeling of nuclei inside muscle fibers or electrocytes (Figure 9C) showing that these cells did not dedifferentiate via reentry into the cell cycle. Instead, BrdU incorporation observed was in cells outside the plasma membrane of muscle fibers and electrocytes (Figure 9C, arrows). There were few intact electrocytes that were immunolabeled with the anti-sarcomeric MHC antibody MF20 (Figure 9D) manifesting an earlier stage in their differentiation from skeletal muscle fibers. However, these MF20-positive electrocytes did not show any other evidence of a dedifferentiation process beyond the re-expression of MyHC.
Figure 9. No evidence of cell dedifferentiation.
Portions of longitudinal cryosections (14-µm thick) from intact tails (A and D) and tails 4 (B) and 14 days (C) after amputation. Tissue sections were immunoreacted with anti-laminin (A and B) to study changes in basal lamina morphology that may indicate cellularization (arrows), anti-BrdU (C) to investigate myonuclei proliferation after tail cut (arrow), and anti-myosin heavy chain (D, red) to examine changes in muscle protein expression in intact mature electrocytes proximal to the tail transection site. Sections in D were co-immunolabeled with anti-Pax7 (green). Abbreviations: EC, electrocyte. Scale bars: 50 µm.doi:10.1371/journal.pone.0036819.g009
To more rigorously test the incidence of cell fragmentation of myogenic tissues during regeneration in S. macrurus, fluorescent dextran was injected into single electrocytes and muscle fibers prior to tail amputation. Longitudinal cryosections of the distal-most region of the ventral fin (50 mm from amputation site) were processed for detection of dextran during the first two weeks after tail amputation. Our in vivo dye-injection studies showed no evidence of cell fragmentation in any of the tails analyzed up to 14 days post-amputation. Electrocytes labeled with single-cell dextran injections showed an intact morphology with no fragmentation into smaller cellular components (Figure 10A). Similar results were obtained with dextran-injected muscle fibers (Figure 10B–C). Small fluorescent remnants of dextran labeling were at times detected around some electrocytes and muscle fibers (Figure 10C), but this fluorescence was not co-labeled with nuclear stain DAPI (Figure 10C–D) indicating these were acellular debris generated by cutting labeled cells. Hence, at least with regard to data from laminin labeling and intracellular dye injection experiments, multinucleated muscle fibers and electrocytes proximal to the injury site did not undergo fragmentation. Hence, these findings are contrary to the association between blastema formation and dedifferentiation of cells near the injury site that has been reported in urodeles , –.
Figure 10. Intracellular tracer dye injections into single muscle fibers and electrocytes at the level of the distal-most ventral fin reveal no cell fragmentation.
Fluororuby (red) was injected into single electrocytes (A) and muscle fibers (B) and visualized 7 days after injection by retracting the overlying skin of the fish. Tail sections at the level of the distal-most region of the ventral fin were processed for immunolabeling and viewed under a fluorescent microscope. (C), region of a cryosection containing muscle fibers injected with Fluororuby dextran (red) and counterstained with DAPI (blue). (D), same image as C showing only DAPI labeling. Scale bars: A and B, 20 µm; C and D, 10 µm.doi:10.1371/journal.pone.0036819.g010
Our cloning analysis revealed that specific amino acids important for transcriptional activation by Pax7 in mammals are also highly conserved in the S. macrurus ortholog (Figure 2). A common feature of the Pax7 gene is the generation of different transcripts through alternative splicing –; –, a feature confirmed for the Pax7 gene in S. macrurus. We identified 2 alternate Pax7 isoforms (GenBank accession numbers EU624121 and EU624122), which varied in size from 300 to 507 amino acid residues, a range similar to that found among Pax7 isoforms reported within different vertebrate species. The number of Pax7 variants confirmed in S. macrurus is fewer than the number of alternate Pax7 transcripts identified in other vertebrates, which range from at least four variants in mice – and zebrafish  to at least 10 variants in other teleosts including Atlantic salmon  and the arctic charr . Hence, it remains to be determined whether the Pax7 gene of S. macrurus encodes additional isoforms, an issue that was beyond the scope of the present study. In addition, establishing whether alternate transcripts are expressed in different cell populations in S. macrurus as it is known in mice and other teleosts would suggest different tissue-specific roles of Pax7 function that are highly conserved in vertebrates.
Our RT-PCR and immunolabeling studies also suggest that expression of Pax7 mRNA and protein, respectively, is highly conserved in S. macrurus. Pax7 transcripts in S. macrurus were mainly found in mature skeletal muscle, electric organ, brain and spinal cord where Pax7 expression was restricted to a subpopulation of neural cells in the dorsal spinal cord. These Pax7 expression patterns in skeletal muscle, brain and spinal cord are similar to those in adult mouse , , urodele amphibians  and other teleosts –. We also show that the commonly used mammalian antibody against Pax7 from the Developmental Hybridoma Bank labeled protein products from in vitro translated Pax7 cDNA and whole tissue homogenates from muscle and electric organ of S. macrurus that correspond to the expected sizes of known Pax7 proteins in mammals and other teleosts (Figure 3B). More importantly, cells associated with muscle fibers and electrocytes with the morphology and location similar to those that define myogenic satellite cells were immunolabeled with the Pax7 antibody (Figure 3C). By combining nuclear Pax7 expression with morphological observations of satellite cells by electron microscopy we conclude that Pax7 is a reliable satellite cell marker in S. macrurus muscle and electric organ. Other molecules like c-Met and M-cadherin have been used to identify satellite cells by other investigators –. However, unlike the Pax7 antibody, antibodies against these other markers did not cross-react successfully with S. macrurus tissues. Pax7 is a commonly used marker for identifying quiescent satellite cells , . In control intact fish, Pax7-positive cells associated with electrocytes and muscle fibers rarely incorporated BrdU (Figure 3E and Figure 6E) suggesting a predominant state of quiescence. Following tail amputation, only some Pax7-positive cells proximal to the amputation site were BrdU positive, and the low incidence of Pax7-BrdU-positive co-labeling was similar regardless of whether satellite cells were associated with, or free of, electrocytes and muscle fibers (Figure 7). These data may reflect the BrdU injection paradigm used for this timepoint wherein, in all likelihood, was not incorporated into all cells that re-entered cell division in response to tail amputation.
Since the first description in frog muscle by Mauro in 1961 , satellite cells have been identified in many vertebrate species and have been considered distinct progenitor cells with the unique ability of associating with, generating, repairing, and maintaining skeletal muscle. This muscle-specific fate has recently been challenged by studies showing that satellite cells can adopt different cell phenotypes –. In each of these cases, satellite cells have been taken away from their natural niche and cultured under conditions to promote the conversion of satellite cells into cells with adipocyte and osteocyte –, and even some neuronal characteristics . Yet, we are not aware of any studies wherein satellite cells show this cell fate potential in vivo and/or demonstrate the occurrence of satellite cells in mature fat, bone or neuronal tissues. In contrast, the association of satellite cells with mature electrocytes is a natural occurrence that represents an exception to the tissue-specificity of these myogenic stem cells to striated muscle fibers. A major quest of future studies is to determine whether the Pax7-positive cells in muscle and electric organ of S. macrurus form a single multipotent class of cells or whether they represent discrete satellite cells for muscle and electrocyte lineages. One way to explore this is to replicate differentiation of muscle fibers and electrocytes in culture. Our ability to isolate satellite cells from muscle fibers or electrocytes will allow us to directly test the potential of these myogenic cells under suitable differentiation conditions –. It will be interesting to find out if satellite cell populations isolated from either tissue reflect their cell type of origin as has been observed with satellite cell populations that give rise to either slow or fast muscle fibers in birds and rodents – – or not, as it has been reported in humans . Our present characterization of satellite cells in muscle and electric organ of S. macrurus represent a modest beginning of a large-scale project that ultimately includes understanding how the skeletal muscle phenotype is transformed to acquire new functions and how this may occur independently in multiple lineages in teleosts.
We studied the response of Pax7-positive cells following amputation, blastema formation, and regeneration of muscle fibers and electrocytes. Upon tail amputation, Pax7 positive cells associated with intact electrocytes and muscle fibers proliferated profusely (Figure 7). Pax7-positive cells associated with electrocytes and muscle fibers that were damaged by tail amputation were also observed to extend in narrow strip-like formations into the blastema (Figures 3A, 5C–D). Within the blastema, the incorporation of Pax7-positive cells into developing dorsal spinal cord, muscle fibers and electrocytes was unmistakable (Figures 4 and 5). This cellular distribution corresponds to tissues which Pax7-positive cells manifest in adult intact tails of S. macrurus (Figure 3). Hence, with regards to the incorporation of Pax7-positive cells into myogenic tissues, our results demonstrate that muscle and electrocyte regeneration in adult S. macrurus is similar to the satellite-dependent muscle repair process common in vertebrates including mammals, frogs, birds, fish and some salamanders [9,55–56,å68–70]. That skeletal muscle regeneration in S. macrurus shows considerable dependence on activation of myogenic Pax7-positive stem cells is further supported by results obtained from our ventral muscle amputation experiments that showed a slower regeneration process associated with considerably fewer changes in Pax7-positive cell number (Figure 8) compared to that observed after tail amputation (Figures 5 and 7).
It has been well documented that formation of a blastema can result from the dedifferentiation of mature tissues proximal to the amputation site –, . Muscle dedifferentiation can involve the reentry of myonuclei into the cell cycle, cellularization or fragmentation of muscle fibers, and/or the loss of muscle-specific proteins, such as, sarcomeric proteins. Our morphological analysis of muscle fibers and electrocytes did not detect evidence of fragmentation of these myogenic cells that was indisputable when compared to control muscle and electrocytes (Figure 9). Our experiments also do not support the occurrence of myonuclei reentering the cell cycle since BrdU incorporation was only detected in satellite cells (Figure 9C). We did observe a few intact electrocytes near the amputation site that re-expressed sarcomeric myosin heavy chain (Figure 9D) indicating a partial reversal in their myogenic phenotype. However, our single-cell tracer dye injection experiments provide the strongest evidence against in vivo fragmentation or cellularization of either muscle fibers or electrocytes in response to tail amputation (Figure 10). Data from these intracellular dye injection experiments together with our Pax7 expression studies suggest that skeletal muscle and muscle-derived electrocytes are regenerated in S. macrurus by a mechanism similar to the myogenic stem cell-dependent muscle repair process seen in mammals. Hence, muscle dedifferentiation is likely one regeneration strategy, but not the requirement in the myogenic lineage of the adult teleost S. macrurus. In sum, this first characterization of the cellular mechanisms involved in the regeneration of myogenic tissues of the adult tail in S. macrurus: 1) demonstrates the important implications that studying regeneration in different animals with robust regeneration potential have on our understanding of the evolution of regeneration processes, 2) support the emergent concept in vertebrate regeneration that different tissues provide a distinct progenitor cell population to the regeneration blastema, and these progenitor cells subsequently restore the original tissue , and 3) represent an exciting starting point in the comparative investigation of regeneration mechanisms underlying the restoration of myogenically derived, highly specialized non-contractile tissues in other teleosts.
We are grateful to Vince Gutschick for live fish photography, Shannon Manuelito and Amanda Meenach for technical support in confocal microscopy, and Jung Kim for assistance in image processing.
Conceived and designed the experiments: GAU MQM SJT. Performed the experiments: CMW MQM GAU. Analyzed the data: CMW GAU. Contributed reagents/materials/analysis tools: MQM GAU. Wrote the paper: GAU CMW.
- 1. Carlson BM (2005) Some principles of regeneration in mammalian systems. Anat Rec 287B: 4–13.
- 2. van Bekkum DW (2004) Phylogenetic aspects of tissue regeneration: role of stem cells. A concise overview Blood Cells Mol Dis 32: 11–6.
- 3. Fausto N, Riehle KJ (2005) Mechanisms of liver regeneration and their clinical implications. J Hepatobiliary Pancreat Surg 12: 181–9.
- 4. Charge SB, Rudnicki MA (2004) Cellular and molecular regulation of muscle regeneration. Physiol Rev 84: 209–38.
- 5. Grounds MD, White JD, Rosenthal N, Bogoyevitch MA (2002) The role of stem cells in skeletal and cardiac muscle repair. J Histochem Cytochem 50: 589–610.
- 6. Brockes JP, Kumar A (2002) Plasticity and reprogramming of differentiated cells in amphibian regeneration. Nat Rev Mol Cell Biol 3: 566–74.
- 7. Nye HL, Cameron JA, Chernoff EA, Stocum DL (2003) Regeneration of the urodele limb: A review. Dev Dyn 226: 280–94.
- 8. Tsonis PA (2000) Regeneration in vertebrates. Dev Biol 221: 273–284.
- 9. Morrison JI, Lööf S, He P, Simon A (2006) Salamander limb regeneration involves the activation of a multipotent skeletal muscle satellite cell population. J Cell Biol 172: 433–440.
- 10. Tanaka EM, Reddien PW (2011) The cellular basis for animal regeneration. Dev Cell 21: 172–185.
- 11. Brockes JP, Kumar A (2005) Appendage regeneration in adult vertebrates and implications for regenerative medicine. Science 310: 1919–1923.
- 12. Echeverri K, Tanaka EM (2002) Mechanisms of muscle dedifferentiation during regeneration. Semin Cell Dev Biol 13: 353–60.
- 13. Stocum DL (2004) Amphibian regeneration and stem cells. Curr Top Microbiol Immunol 280: 1–70.
- 14. Duckmanton A, Kumar A, Chang Y-T, Brockes JP (2005) A single-cell analysis of myogenic dedifferentiation induced by small molecules. Chem Biol 12: 1117–1126.
- 15. Kumar A, Velloso CP, Imokawa Y, Brockes JP (2000) Plasticity of retrovirus-labelled myotubes in the newt limb regeneration blastema. Dev Biol 218, 125–136:
- 16. Bennett MVL (1971) Electric Organs. In: Hoar WS, Randall DJ, editors. Fish Physiology. Academic Press. pp. 347–491.
- 17. Baillet-Derbin , C (1978) Cytodifferentiation of the regenerating electrocyte in an electric Fish. Biol Cell 33: 15–24.
- 18. Kirschbaum F, Meunier FJ (1981) Experimental regeneration of the caudal skeletal of the glass knifefish Eigenmania virescens J Morphol 168: 121–135.
- 19. Kirschbaum F, Meunier FJ (1988) South American gymnnotiform fishes as model animals for regeneration experiments. Monogr Dev Biol 21: 112–123.
- 20. Meunier FJ, Kirschbaum F (1979) Comparative anatomy of the axial caudal skeleton of Gymnotoidei. Third European Ichthyological Congress, Warsaw.
- 21. Patterson JP, Zakon HH (1993) Bromodeoxyuridine labeling reveals a class of satellite-like cells within the electric organ. J Neurobiol 24: 660–674.
- 22. Sîrbulescu RF, Ilies? I, Zupanc GK (2009) Structural and functional regeneration after spinal cord injury in the weakly electric teleost fish, Apteronotus leptorhynchus. J Comp Physiol A Neuroethol Sens Neural Behav Physiol 195: 699–714.
- 23. Zakon HH (1991) Regeneration of electroreceptors in weakly electric fish. Ciba Found Symp 160: 294–308.
- 24. Anderson MJ, Waxman GG (1981) Morphology of regenerated spinal cord in Sternarchus albifrons. Cell Tiss Res 219: 1–8.
- 25. Zakon HH, Unguez GA (1999) Development and regeneration of the electric organ. J Exp Biol 202: 1427–1434.
- 26. Brusca RC, Brusca GJ (1990) Invertebrates. Sunderland, Mass: Sinauer Associates, Inc.
- 27. Alvarado AS (2000) Regeneration in the metazoans: why does it happen?. BioEssays 22: 578–590.
- 28. Unguez GA, Zakon HH (1998) Phenotypic conversion of distinct muscle fiber populations to electrocytes in a weakly electric fish. J Comp Neurol 399: 20–34.
- 29. Unguez GA, Zakon HH (2002) Skeletal muscle transformation into electric organ in S. macrurus depends on innervation. J Neurobiol 53: 391–402.
- 30. Seale P, Sabourin LA, Girgis-Gabardo A, Mansouri A, Gruss P, Rudnicki MA (2000) Pax7 is required for the specification of myogenic satellite cells. Cell 102: 777–786.
- 31. Kim JA, Laney C, Curry J, Unguez GA (2008) Expression of Myogenic Regulatory Factors in the Muscle Derived Electric Organ of Sternopygus macrurus. J Exp Biol 211: 2172–2184.
- 32. Cuellar H, Kim JA, Unguez GA (2006) Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus. FASEB J 20: E1856–E1865.
- 33. Kim JA, Jonsson CB, Calderone T, Unguez GA (2004) Transcription of MyoD and myogenin in the non-contractile electrogenic cells of the weakly electric fish S. macrurus. Dev Genes Evo 214: 380–392.
- 34. Choi D, Li D, Raisman G (2002) Fluorescent retrograde neuronal tracers that label the rat facial nucleus: a comparison of Fast Blue, Fluoro-ruby, Fluoro-emerald, Fluoro-gold and DiI. J Neurosci Methods 117: 167–172.
- 35. Shimotori T, Goto T (2001) Developmental fates of the first four blastomeres of the chaetognath Paraspadella gotoi: relationship to protostomes. Dev Growth Differ 43: 371–82.
- 36. Bolte S, Corderlieres FP (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Micros 224: 213–232.
- 37. Seo HC, Saetre BO, Håvik B, Ellingsen S, Fjose A (1998) The zebrafish Pax3 and Pax7 homologues are highly conserved, encode multiple isoforms and show dynamic segment-like expression in the developing brain. Mech Dev 70S: 49–63.
- 38. Ziman MR, Fletcher S, Kay PH (1997) Alternate Pax7 transcripts are expressed specifically in skeletal muscle, brain and other organs of adult mice. Int J Biochem Cell Biol 29: 1029–36.
- 39. Jostes B, Walther C, Gruss P (1990) The murine paired box gene, Pax7, is expressed specifically during the development of the nervous and muscular system. Mech Dev 33: 27–37.
- 40. Schultz E, Chamberlain C, McCormick KM, Mozdziak PE (2006) Satellite cells express distinct patterns of myogenic proteins in immature skeletal muscle. Dev Dyn 235: 3230–3239.
- 41. Ishido , Uda M, Kasuga M, Masuhara N, M (2008) The expression patterns of Pax7 in satellite cells during overload-induced rat adult skeletal muscle hypertrophy. Acta Physiologica 195: 459–469.
- 42. Chen Y, Lin GF, Slack JMW (2006) Control of muscle regeneration in the Xenopus tadpole tail by Pax7. Dev 133: 2303–2313.
- 43. Steinbacher P, Haslett JR, Obermayer A, Marschallinger J, Bauer HC, et al. (2007) MyoD and Myogenin Expression During Myogenic Phases in Brown Trout: A precocious onset of mosaic hyperplasia is a prerequisite for fast somatic growth. Dev Dyn 236: 1106–1114.
- 44. Halevy O, Piestun Y, Allouh MZ, Rosser BWC, Rinkevich Y, et al. (2004) Pattern of Pax7 expression during myogenesis in the posthatch chicken establishes a model for satellite cell differentiation and renewal. Dev Dyn 231: 489–502.
- 45. McHedlishvili L, Epperlein HH, Telzerow A, Tanaka EM (2007) A clonal analysis of neural progenitors during axolotl spinal cord regeneration reveals evidence for both spatially restricted and multipotent progenitors. Dev 134: 2083–2093.
- 46. Schnapp E, Tanaka EM (2005) Quantitative evaluation of morpholino-mediated protein knockdown of GFP, MSX1, and PAX7 during tail regeneration in Ambystoma mexicanum. Dev Dyn 232: 162–70.
- 47. Kawakami A, Kimura-Kawakami M, Nomura T, Fujisawa H (1997) Distributions of PAX6 and PAX7 proteins suggest their involvement in both early and late phases of chick brain development. Mech. Dev 66, 119–130:
- 48. Brockes JP (1998) Progenitor cells for regeneration: dedifferentiation and immortaliasation. In: Ferretti P, Geraudie J, editors. Cellular and Molecular Basis of Regeneration; from Invertebrates to Humans. John Wiley and Sons Limited. pp. 63–77.
- 49. Echeverri K, Clarke JD, Tanaka EM (2001) In vivo imaging indicates muscle fiber dedifferentiation is a major contributor to the regenerating tail blastema. Dev Biol 236: 151–64.
- 50. Lo DC, Allen F, Brockes JP (1993) Reversal of muscle differentiation during urodele limb regeneration. Proc Natl Acad Sci 90: 7230–7234.
- 51. Kumar A, Velloso CP, Imokawa Y, Brockes JP (2004) The regenerative plasticity of isolated urodele myofibers and its dependence on Msx1. PLoS Biol 2: E218.
- 52. Gotensparre SM, Andersson E, Wargelius A, Hansen T, Johnston IA (2006) Insight into the complex genetic network of tetraploid Atlantic salmon (Salmo salar L.): description of multiple novel Pax-7 splice variants. Gene 373: 8–15.
- 53. Sibthorpe D, Sturlaugsdóttir R, Kristjansson BK, Thorarensen H, Skúlason S, Johnston IA (2006) Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr (Salvelinus alpinus). Comp Biochem Physiol B Biochem Mol Biol 145: 371–8.
- 54. Cornelison DD, Wold BJ (1997) Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells. Dev Biol 191: 270–83.
- 55. Hawke TJ, Garry DJ (2001) Myogenic satellite cells: physiology to molecular biology. J Appl Physiol 91: 534–51.
- 56. Zammit PS, Golding JP, Nagata Y, Hudon V, Partridge TA, Beauchamp JR (2004) Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? J Cell Biol 166: 347–357.
- 57. Mauro A (1961) Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 9: 493–495.
- 58. Asakura A, Komaki M, Rudnicki M (2001) Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68: 245–253.
- 59. Singh NK, Chae HS, Hwang IH, Yoo IM, Ahn CN, et al. (2007) Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone. J Anim Sci 85: 1126–1135.
- 60. Wada MR, Inagawa-Ogashiwa M, Shimizu S, Yasumoto S, Hashimoto N (2002) Generation of different fates from multipotent muscle stem cells. Dev 129: 2987–95.
- 61. Redshaw Z, McOrist S, Loughna P (2010) Muscle origin of porcine satellite cells affects in vitro differentiation potential. Cell Biochem Funct 28: 403–411.
- 62. Archer E, Escobedo N, Kim HJ, Gabillard J-C, Unguez GA (2008) Differential potential of primary myogenic cells derived from adult muscle and electric organ of the gymnotiform S. macrurus. Abstract Viewer/Itinerary Planner. Society for Developmental Biology.
- 63. Shadd V, Archer E, Escobedo , N , Kim HJ, Unguez GA (2009) Culture of Myogenic Cells Derived from Adult Myogenic Tissues and Regeneration Blastema of the Gymnotiform S. macrurus. Abstract Viewer/Itinerary Planner. Society for Developmental Biology.
- 64. Feldman JL, Stockdale FE (1991) Skeletal muscle satellite cell diversity: satellite cells form fibres of different types in culture. Dev Biol 143: 320–334.
- 65. Rosenblatt JD, Parry DJ, Partridge TA (1996) Phenotype of adult mouse muscle myoblasts reflects their fibre type of origin. Differentiation 60: 39–45.
- 66. Dusterhoft S, Pette D (1993) Satellite cells from slow rat muscle express slow myosin under appropriate culture conditions. Differentiation 53: 25–33.
- 67. Bonavaud S, Agbulut O, Nizard R, D’honneur G, Mouly V, Butler-Browne G (2001) A discrepancy resolved: human satellite cells are not preprogrammed to fast and slow lineages. Neuromuscular Disorders. 11: 747–752.
- 68. Morrison JI, Borg P, Simon A (2010) Plasticity and recovery of skeletal muscle satellite cells during limb regeneration. FASEB J 24: 750–756.
- 69. Feldman JL, DiMario JX, Stockdale FE (1993) Developmental appearance of adult myoblasts (satellite cells): studies of adult myoblasts in culture and adult myoblast transfer into embryonic avian limbs. Prog Clin Biol Res 383B: 563–74.
- 70. Gabillard JC, Sabin N, Paboeuf G (2010) In vitro characterization of proliferation and differentiation of trout satellite cells. Cell Tissue Res 342: 471–7.
- 71. Nechiporuk A, Keating MT (2002) A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration. Dev 129: 2607–2617.