Figures
Abstract
There is a long-standing debate on the extent of vicariance and long-distance dispersal events to explain the current distribution of organisms, especially in those with small diaspores potentially prone to long-distance dispersal. Age estimates of clades play a crucial role in evaluating the impact of these processes. The aim of this study is to understand the evolutionary history of the largest clade of macrolichens, the parmelioid lichens (Parmeliaceae, Lecanoromycetes, Ascomycota) by dating the origin of the group and its major lineages. They have a worldwide distribution with centers of distribution in the Neo- and Paleotropics, and semi-arid subtropical regions of the Southern Hemisphere. Phylogenetic analyses were performed using DNA sequences of nuLSU and mtSSU rDNA, and the protein-coding RPB1 gene. The three DNA regions had different evolutionary rates: RPB1 gave a rate two to four times higher than nuLSU and mtSSU. Divergence times of the major clades were estimated with partitioned BEAST analyses allowing different rates for each DNA region and using a relaxed clock model. Three calibrations points were used to date the tree: an inferred age at the stem of Lecanoromycetes, and two dated fossils: Parmelia in the parmelioid group, and Alectoria. Palaeoclimatic conditions and the palaeogeological area cladogram were compared to the dated phylogeny of parmelioid. The parmelioid group diversified around the K/T boundary, and the major clades diverged during the Eocene and Oligocene. The radiation of the genera occurred through globally changing climatic condition of the early Oligocene, Miocene and early Pliocene. The estimated divergence times are consistent with long-distance dispersal events being the major factor to explain the biogeographical distribution patterns of Southern Hemisphere parmelioids, especially for Africa-Australia disjunctions, because the sequential break-up of Gondwana started much earlier than the origin of these clades. However, our data cannot reject vicariance to explain South America-Australia disjunctions.
Citation: Amo de Paz G, Cubas P, Divakar PK, Lumbsch HT, Crespo A (2011) Origin and Diversification of Major Clades in Parmelioid Lichens (Parmeliaceae, Ascomycota) during the Paleogene Inferred by Bayesian Analysis. PLoS ONE 6(12): e28161. https://doi.org/10.1371/journal.pone.0028161
Editor: Robert DeSalle, American Museum of Natural History, United States of America
Received: September 28, 2011; Accepted: November 2, 2011; Published: December 8, 2011
Copyright: © 2011 Amo de Paz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by funds of the Ministerio de Ciencia e Innovación (CGL2010-21646), a Ramón y Cajal grant (RYC02007-01576) to PKD, and a grant of the National Science Foundation to HTL (“Hidden diversity in parmelioid lichens”, DEB-0949147). The Universidad Complutense de Madrid is thanked for providing all complementary facilities. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
In traditional, morphology-based concepts, lichenized fungi often have wide distributions spanning over several continents with a number of species being cosmopolitan. This has led to a widespread notion that the distribution of these fungi is primarily shaped by ecological conditions rather than explained by historical events. In contrast, a few authors have invoked plate tectonics and emphasized vicariance to explain distribution patterns of lichens, especially for species occurring in the Southern Hemisphere [1]–[6]. However, within the last decade, molecular data have helped to revolutionize the species delimitation of lichenized fungi and demonstrated that morphology-based concepts largely underestimate the diversity of lichens [7]–[16]. As a result of these studies, it became clear that – although the number of widely distributed species in lichenized fungi is generally higher than in plants or animals – lichens have more restricted distribution areas than previously assumed. For example, supposedly cosmopolitan species in Parmeliaceae and Physciaceae were found to represent distinct lineages in different continents [11], [13]. Further, recent progress in our knowledge of the phylogeny of some clades of lichenized fungi revealed the presence of clades at generic rank that originated and diversified in the Southern Hemisphere [17]–[21]. Hence, we have turned our attention to address the issue of the extent of vicariance and long-distance dispersal to understand the current distribution of lichenized fungi anew using molecular phylogenies.
For this purpose a dated phylogeny with the estimated ages of origin and diversification of the parmelioid group is required. A main problem for building dated phylogenies in fungi is the poor fossil record. While our understanding of the divergence time of angiosperms is well established [22]–[25],. time estimates for fungi were long disputed based on uncertainties in the interpretation of the few known fossils [26]. Consequently, published dating estimates ranged from 660 million to 2.15 billion years ago for the origin of Fungi and from 390 million to 1.5 billion years for the split of the two crown groups of fungi, Ascomycota and Basidiomycota [27]–[31]. Re-examination of the morphology of the fossils and re-evaluation of the published dating studies, however, suggested more consistent results with the origin of the Fungi dating back to between 760 million and 1.06 billion years, and the split of Ascomycota and Basidiomycota for about 500–650 Ma [26], [32]. This suggests that terrestrial fungi evolved and diversified more or less simultaneously with the evolution of land plants.
Given that the phylogeny of parmelioid lichens is well resolved [18] and the recent advantages in our understanding of the timing of major events in the evolution of fungi, we feel confident that the times of main nodes differentiation can be estimated. Moreover, this dating approach can be applied to address the issue of the extent of vicariance on distribution patterns in lichens and use parmelioids as an example.
The family Parmeliaceae is widely distributed throughout the world from polar to tropical regions and is one of the largest families of lichenized Ascomycota [18], [33]–[36]. Most species form foliose or fruticose thalli, but some also have subcrustose, umbilicate, peltate thalli, and even lichenicolous fungi were found to belong here [18], [35], [37]. The family includes about 2500 species classified in 84 genera and is characterized by cup-shaped apothecia with cupulate exciple, Lecanora-type asci, often with hyaline ascospores [35]. Within the family, six strongly supported major monophyletic groups can be distinguished [35], which are: alectorioid, cetrarioid, hypogymnioid, letharioid, parmelioid and psiloparmelioid. By far, the largest of these groups is the parmelioid group with about 1500 species [38], [39] classified in 27 accepted genera [18]. Phenotypically, the parmelioid lichens are characterized by having mostly foliose thalli with thread-like rhizines on the lower surface, cup-shaped apothecia on the thallus upper surface and Lecanora-type asci with hyaline ascospores (Fig. 1). Within the parmelioid lichens, eight major monophyletic clades can be distinguished, which are Cetrelia-, Hypotrachyna-, Melanohalea-, Parmelia-, Parmelina-, Parmeliopsis-, Parmotrema- and Xanthoparmelia-groups [18]. During the last decades, phylogenetic studies based on DNA sequence data have greatly advanced our understanding of the evolution of the family including the phylogenetic relationships among major clades [18], [34]–[35], [40]–[46], phenotypical evolution [35], [45], [47], disparity in substitution rates among clades [48], and the geographic origin of certain clades [17], [19]. However, so far none of these have aimed at dating major cladogenesis events in Parmeliaceae. Besides the issues discussed above, this is probably also due to the poor fossil record of Parmeliaceae. Only very few fossils have been recorded of Parmeliaceae and the known ones are all preserved in amber. An Alectoria species was described from Baltic amber (35–40 Ma) [49]. Two fossil species of Parmelia were described from Dominican amber (15–45 Ma) [50] and two specimens of Anzia have been found from European amber (35–40 Ma) [51].
A. Parmelina tiliacea, one of the most common lichens in the Mediterranean basin. B. Parmotrema hypoleucinum, endemic of the southwestern Mediterranean Region, occurring in warm and humid areas. C. Melanelixia glabra occurs from southwestern Europe to eastern Russia. D. Parmelia sulcata, a common species from cold to temperate regions of both Hemispheres. E. Flavoparmelia soredians occurs in warm and humid areas of temperate regions of both Hemispheres. F. Xanthoparmelia conspersa, one of the most widespread species of macrolichens growing on acid rocks in temperate areas of both Hemipheres, excluding Australia and South Africa. The distribution areas after Nimis [105]. All photographs were taken in the field. Scale = 1 cm.
The aims of this study are 1) to put a time-scale on the phylogenetic tree of parmelioid lichens and thus identify when the main nodes differentiated, employing calibration points from a recent dating estimate of fungi [26] and available fossil reference-points, and 2) to address the impact of vicariance and long-distance dispersal processes in the distribution patterns in parmelioid lichens. Specifically we address the question whether plate tectonics can be invoked to explain distribution areas of species groups or species occurring in the Southern Hemisphere.
Results
Data and substitution patterns
For the analyses a dataset of three loci of 225 OTUs was used. The matrix included 1849 unambiguously aligned nucleotide characters, with 599 positions in the RPB1gene, 655 positions in the nuLSU and 595 positions in the mtSSU. The number of constant characters was 643. The likelihood value of the ML tree obtained with Garli was lnL = −59525.412. The constrained position of Chaethothyriomycetidae as sister group of Lecanoromycetes is not significantly worse than the unconstrained topology (p-SH = 0.444, c-ELW = 0.441).
The substitution rates of the three loci (nuLSU, mtSSU and RPB1) are shown for each lineage of the parmelioid lichens in Fig. 2 and Table 1. The different lineages have similar range of variation in each gene but there are clear differences in mean rates between the three genes, the rate of RPB1 being two to four times higher than mtSSU and nuLSU, respectively.
Dots represent mean rates and bars cover the 95% highest posterior density (HPD). Units: substitution/site/year. Abbreviations for the Parmelioid clades are listed in Table 1.
Estimating divergence times
A chronogram based on the analysis of the combined matrix of three loci is shown in Fig. 3, showing the relationships of Lecanoromycetes and Parmeliaceae (highlighted in dark grey color). The detailed chronogram for Parmeliaceae is depicted in Fig. 4. This analysis used as calibration points the age of the split of Lecanoromycetes-Chaethothyriomycetidae (C1); the age of the fossil (P. ambra) assigned to the Parmelia s.s. crown node (C2), and the age of the fossil A. succini assigned to the Alectoria crown (C3). The node ages, 95% highest posterior density intervals (HPD) of ages, substitution rates and 95% HPD of substitution rates for the main clades, divergence points and diversification point of the genera are shown in Table 2.
The chronogram was estimated from a partitioned data set of three loci (mtSSU, nuLSU, RPB1) using BEAST. The calibration point (C1) was set at the divergence node of Lecanoromycetes and Chaethothyriomycetidae.
The chronogram was estimated as described in the legend for figure 3. Two calibration points based on fossil assignation are marked as C2 (crown node of Parmelia) and C3 (crown node of Alectoria). C3* is the alternative calibration with the Alectoria fossil assigned to the alectorioid crown. The Parmelioid group is highlighted by the grey box. Numbers inside circles refer to divergence nodes between the main clades. Grey bars show the 95% highest posterior density intervals (HPD). Detailed ages are given in Table 2.
The alternative analyses gave similar results (Table 3). The small differences are as follows:1) using two calibration points (C1 and C2) and excluding the Alectoria fossil, node ages and 95% HPD intervals are slightly younger for some of the parmelioid clades; 2) using C1 and C3 and excluding Parmelia, the node ages and 95% HPD intervals had a small decrease in older clades; and 3) using C1, C2 and the Alectoria fossil constraining the age of the alectorioid clade (Alectoria, Bryoria and Pseudephebe; C3*) the node ages are similar with very small fluctuations. The 95% HPD intervals for all the clades largely agree and are similar to those obtained in the first analysis using the calibration points C1, C2 and C3.
The split of the Parmeliaceae core was estimated to be around 109 Ma (85.52–136.55 Ma) when the crustose genus Protoparmelia separated from the rest of the Parmeliaceae (Fig. 4, Table 2). The basal radiation of Parmeliaceae core took place between 60 and 74 Ma, when the main lineages of the family originated. The radiation of the large group of Parmelioid lichens was estimated to have begun in the Paleocene, around 60 Ma (49.81–73.55 Ma). The morphologically close but phylogenetically distant cetrarioid group, mostly distributed in temperate to alpine regions, was estimated to radiate in the Eocene (27 Ma, 18.81–37.90 Ma, Fig. 4) while the alectorioid clade radiated about 47 Ma (40.88–54.97 Ma).
Our analyses suggest seven separate major divergence events that led to the evolution of the main clades of parmelioid lichens (Fig. 4, marked with dots and numbers). The earliest divergence, estimated around 48 Ma, separated the Xanthoparmelia-clade from the Parmotrema-clade (including the genera Austroparmelina, Canoparmelia, Flavoparmelia, Punctelia, Flavopunctelia, Nesolechia and Parmotrema). In subsequent divergence events during Eocene time Melanohalea split from Melanelixia (about 42 Ma), Parmelina separated from Myelochroa (37 Ma), Remototrachyna from Bulbothrix (about 37 Ma) and Austroparmelina differentiated from the most recent common ancestor (MRCA) of Parmotrema, Flavoparmelia and Canoparmelia crozalsiana-clade (about 34 Ma). During the early Oligocene Cetrariastrum (about 32 Ma) differentiated from the rest of the complex Hypotrachyna-clade (including Everniastrum, Hypotrachyna s.l., and Parmelinopsis), and. Flavoparmelia separated from Parmotrema and Canoparmelia crozalsiana-clade (30 Ma).
Our data indicate that diversification of the recent genera of parmelioid lichens occurred during Oligocene and Miocene. The diversification of the Melanelixia was estimated to be around (34 Ma), and in a second radiation event (22–25 Ma) diversified Melanohalea, Parmelia, Remototrachyna, Flavoparmelia and Xanthoparmelia. Myelochroa, Punctelia and Parmotrema radiated during the Miocene (between 14 and 18 Ma), and Cetrelia, Parmelina and Canoparmelia crozalsiana-clade was estimated to radiated by late Miocene and early Pliocene (4–9 Ma).
The estimated ages of diversification of the main clades of parmelioid lichens compared to the geological area cladogram representing the relationships among the Southern Hemisphere landmasses are shown in Figure 5. The break-up of the main landmasses of the South Hemisphere predate the ages of diversification of the parmelioid clades and the splits of the main lineages (Fig. 5). The presence of several genera (e.g. Xanthoparmelia, Parmotrema, Bulbothrix, Austroparmelina, Flavoparmelia, Melanelixia, Myelochroa, Hypotrachyna, Punctelia) in different continents of the Southern Hemisphere (A and B, Fig. 5) cannot be explained by continental drift and vicariance because when the parmelioid lineages started to diverge (60 Ma) Africa had already separated from South America, India, New Zealand and Australia.
Dots represent node ages and bars cover the 95%HPD. The geological area cladogram representing the relationships among the Southern Hemisphere landmasses is based on Sanmartín & Ronquist [106].
Discussion
In this study we put a time-scale on the phylogenetic tree of parmelioid lichens using three DNA regions with different evolutionary rates. The estimated ages allow addressing how vicariance and long-distance dispersal shaped the current distribution patterns of parmelioid lichens, specially the disjunct distributions of species groups in the Southern Hemisphere. Moreover the dated phylogeny provides a general picture of the palaeoclimatic conditions prevalent on Earth when the main lineages differentiated.
The three DNA regions used in this study to build the phylogenetic trees have different evolutionary rates. We found higher substitution rates in the protein coding gene RPB1 than in the nuLSU and mtSSU ribosomal DNA (Fig. 2, Table 2). Ribosomal DNA has been frequently used in molecular studies of Parmeliaceae and other lichenized fungi [34], [41]–[42], [44]–[47], [52]–[54] but so far few molecular studies have used protein-coding genes to infer phylogenetic relationships in Parmeliaceae [18], [35], [43], [55]–[56]. In our analysis the RPB1 gene (with high substitution rates) provided better resolution of the terminal lineages of the tree while the more conserved genes with lower substitution rate (nuLSU, mtSSU) better supported the backbone of the tree topology.
Substitution rates have been used to estimate divergence times due to the lack of fossil records. Takamatsu & Matsuda [57] calculated a substitution rates for Erysiphales (2.52×10−9 s/s/y for nuLSU). Our estimation of the substitution rate for the nuLSU of the Parmeliaceae (1.12×10−9 s/s/y; Table 2) is in the same order of magnitude but slower than in the phytopathogenous fungi of Erysiphales. This indicates that caution should be used when applying substitution rates of a group of fungi to estimate divergence times of an unrelated group [58]–[59].
The scarcity and uncertainty of the fossil record was a major obstacle for estimation of dates of radiation in most groups of fungi. We have estimated divergence times of the major lineages of Parmeliaceae and times of the main radiations of the genera using a comprehensive phylogenetic hypothesis of the family, calibrated with the available fossil evidence, a root time inferred from [26], and allowing a relaxed-clock model for the rates of evolution of the main clades.
Berbee & Taylor [32] estimated the age of the parmeliaceae crown node at about 60 Ma based on substitution rates, and a minimum age of the family was given at about 40 Ma according to fossil records [50]–[51]. However, the age estimates obtained herein suggested that Parmeliaceae evolved much earlier. Our analyses indicate that the Parmeliaceae core originated rather recently with a stem node age estimate of 108 Ma (Fig. 3, 4, Table 2) and a crown node age estimate of 74 Ma (Fig. 4, Table 2). For other major families of lichenized ascomycetes much older crown node ages have been estimated (Rivas Plata & Lumbsch, pers. com.), including Graphidaceae (156 Ma), Physciaceae (153 Ma), and Ramalinaceae (126 Ma). The parmelioid clade is the largest and most strongly supported monophyletic group of the family. Within the parmelioid crown a total of seven major divergence events at different times have been found (Fig. 4), ranging from early Eocene (separation of Xanthoparmelia from the Parmotrema-clade) to Oligocene (separation of Flavoparmelia from Parmotrema and Canoparmelia crozalsiana-clade). The radiations of parmelioid genera were estimated to start at the end of Eocene (Melanelixia) and occurred during the Oligocene-Miocene for most of the genera.
Major clades of parmelioid lichens either show distinct distribution patterns of the clade or include numerous species with disjunct distributions, as in the genera Xanthoparmelia, Austroparmelina, Melanohalea, Parmelina, and Remototrachyna [11], [17], [19], [41], [60]–[64]. Examples of disjunct distributions include Xanthoparmelia and Austroparmelina. Xanthoparmelia with ca. 800 species, the most speciose clade of Parmeliaceae, occurs worldwide, although in some cases the species delimitation has recently been challenged [65], and in other cases they have been shown much more restricted than previously known [61]–[64]. Despite being cosmopolitan, Xanthoparmelia has two main areas of distribution in arid regions of the Southern Hemisphere (Australia and Africa). A distribution pattern spanning over Australia and Africa cannot be explained by vicariance since these landmasses separated much earlier (Fig. 5; 110–135 Ma) than the split of the Xanthoparmelia lineage from the Parmotrema-clade (37–58 Ma). The data, however, cannot reject vicariance as the reason for distribution ranges of species occurring in South America and Australia. The separation of these continents (35–52 Ma) happened at a similar time as the origin of this lineage.
Austroparmelina includes 13 species that occur in southern and eastern Australia, Tasmania and New Zealand. Two species have wider distribution areas, occurring also in South Africa (A. labrosa, A. pseudorelicina; [18]); and one species also is known from South America (A. labrosa, Chile; [66]). According to our estimates the genus separated from its sister lineages in the late Eocene. Thus the presence of Austroparmelina in South Africa is most plausibly explained as a result of long distance dispersal because separation of Africa took place much earlier (Fig. 5). As in the case of Xanthoparmelia, our data cannot reject vicariance events as an explanation for the presence of A. labrosa in South America and Australia.
In general the estimated ages of diversification events found in our analyses indicate that disjunct distribution patterns of Southern Hemisphere lineages cannot be explained by vicariance. Transoceanic long distance dispersal is the most plausible cause to explain these distribution patterns. This is especially true for taxa occurring in Africa and Australia. This is consistent with recent studies employing molecular clock approaches interpreting distribution patterns in other groups of fungi and plants [58]–[59], [67]–[76], suggesting that long-distance dispersal is an important factor to explain the current distribution patterns of plants and fungi. In the case of lineages distributed at present in South America, Antarctica and Australia the estimated ages do not discard that vicariance could have resulted from the break-up of continents that had occurred 35–52 Ma.
For genera with more restricted distribution ranges and species groups occurring mainly in the Holarctic, additional phylogeographical data are necessary to test biogeographical hypotheses. This is the case e.g., for Parmelina, a genus that occurs in areas with a Mediterranean climate in the Northern Hemisphere [11]. Its separation from Myelochroa, a genus with center of distribution in eastern Asia [77] but extends further into temperate regions, was estimated as having happened in the late Eocene. Similarly the recently described genus Remototrachyna, a Southeast Asian element with only one pantropical species [19], diverged from its sister-lineage Bulbothrix also in the late Eocene.
Our analyses suggest a complex relationship of diversification events and palaeoclimatic conditions. The origin of Parmeliaceae was estimated in the late Cretaceous when the climate was warmer than today, and when subtropical to temperate fauna and flora extended well into polar latitudes [78]–[79]. The first radiation of the parmelioid group (60 Ma) occurred just before the Early Eocene Climatic Optimum (52 Ma) (Fig. 6) when temperature and atmospheric CO2 reached maximum levels [80]. During this time period, the general climate was warm and humid, associated with tectonic changes and volcanism [81]–[82]. Most of the parmelioid lineages, however originated during the Eocene cooling and Oligocene glaciation (Fig. 6). During the Eocene–Oligocene transition (33.5 Ma) a profound global climate shift took place changing the Cretaceous/early Palaeogene “Green House” conditions to “Ice House” conditions, with the growth of Antarctic ice sheets to approximately their modern size, and the appearance of Northern Hemisphere glacial ice [80]. The radiation of the genera took place at different times from the early Oligocene to the Pliocene. Melanelixia started to radiate during the early Oligocene. During this time, the general climate was characterized for cold conditions of the Oligocene glaciation (B, Fig. 6). However Melanohalea, Parmelia, Remototrachyna, Flavoparmelia, Xanthoparmelia and Myelochroa started to radiate between the Late Oligocene Warming. and the Mid-Miocene Climatic Optimum (C–D, Fig. 6). The radiation of other genera (Punctelia, Parmotrema, Cetrelia, Parmelina, Canoparmelia crozalsiana-clade) is estimated as having occurred at the transition from the middle Miocene to the Early Pliocene, before the climate became cooler, drier and seasonal at the end of Pliocene [80].
The global temperature changes obtained from the deep-sea oxygen and carbon isotype proxies after Zachos et al [107].The major diversification events of parmelioid lichens are mapped onto the temperature curve: the square represents the parmelioid most recent common ancestor (MCRA), circles indicate splits of major lineages (numbers in the tree refer to those in Table 2), and diamonds the radiation of genera. Climatic events: A. Early Eocene Climatic Optimum. B. Oi-1 Glaciation. C. Late Oligocene Warming. D. Mid-Miocene Climatic Optimum.
Conclusions
Using three calibration points (one at the split of Lecanoromycetes and Eurotiomycetes, and the ages of two fossil lichens) we obtained the first dated phylogeny of parmelioid lichens and estimated the ages of divergence of the well-resolved lineages and main genera. The radiation of the Parmelioid occurred near the Cretaceous-Tertiary (K/T) boundary, before the climatic optima. These age estimates indicate that long-distance dispersal has played a major role in shaping the current distribution of the Southern Hemisphere parmelioid lichens and that continental drift of Gondwana landmasses and vicariance cannot explain the Africa-Australia disjunct patterns. The major genera originated during Eocene and Oligocene, and radiated during cooling periods at different times from the late Oligocene to early Pliocene.
Materials and Methods
Taxon sampling, sequence alignment and selection of substitution model
A dataset of 225 specimens of Lecanoromycetes with complete sequences of nuLSU rDNA, mtSSU rDNA and the protein coding RPB1 gene, generated in previous studies [18], [83]–[84], was compiled for this study. GenBank accession numbers are listed in Table S1. The parmelioid lineages (the main focus of this work) were represented by 96 OTUs including 24 genera. The major lineages of Lecanoromycetes [85]–[86] and outgroups (Chaethothyriomycetidae and Dothideomycetes), represented by 129 OTUs were included in the analyses to prevent that uneven sampling across the tree could distort the apparent trend in speciation through time [87]. The sequences of each locus were aligned separately using Muscle 3.6 [88] and the ambiguous positions removed using Gblocks with default settings [89]–[90]. The general time reversible model including estimation of invariant sites (GTR+I+G) was selected by jModelTest v 0.1.1 [91]–[92] as the most appropriate nucleotide substitution model for the three separated loci.
Calibration of nodes and dating analysis
The divergence time analyses were performed using BEAST v.1.6.1 [93]. For the dating analysis it is recommended to use a user starting tree instead of the random starting tree built by BEAST. The latter is very likely to violate the temporal and/or topological constraints specified to calibrate divergence times, and cause an error when attempting to initiate the MCMC. For building this tree we checked the phylogenetic signal of our matrix running preliminary ML and Bayesian analyses using Garli 0.96 [94] and MrBayes 3.1.1 [95]. ML analyses were carried out with the default settings and Bayesian analyses were performed assuming a GTR+I+G model, run for 5 million of generations with 4 chains and every 100th tree sampled. The first 5000 generations were burned in and a majority rule consensus tree was calculated with the sumt option. The topologies generated separately for each locus by ML and Bayesian analyses were congruent with the topology of the three loci concatenated, and with the general phylogeny obtained by Crespo et al. [18].
Three points of calibration were used for this study. The principal calibration point (C1) was the divergence time of 280–330 Ma for the stem of Lecanoromycetes following [26]. In addition we used the ages of two fossil lichens: the diversification node (C2) of Parmelia was calibrated with fossils from the Dominican amber (Parmelia ambra, 15–45 Ma, [50]), and the crown node (C3) of Alectoria with a fossil from the Baltic amber (35–40 Ma, [49]). The assignation of fossils to extant groups is a crucial matter in dating analyses, and in the case of the lichens the fossil record is so sparse that this becomes particularly important. Parmelia ambra is a fossil from the Dominican amber resembling Parmelia saxatilis and similar species [50]. It presents unclear terminal pseudocyphellae, elongate isidia, plane to concave upper surface, and simple to dichotomously branched rhizines. All these features are characteristic of the Parmelia s. s. clade, and thus the fossil could also be related to the phylogenetically close genus Relicina (sister group of the Parmelia s.s.), the ‘Parmelia signifera’ group, or to the morphologically close Nipponoparmelia [18]. Relicina was discarded because P. ambra does not have cilia, a feature present on the Relicina species. The species of the ‘Parmelia signifera’ group have subsquarrose rhizines different from the simple to dichotomously branched rhizines of the fossil. On the other hand, Nipponoparmelia presents lobes rolled upwards [96] different from the flat lobes of the fossil. Moreover, only N. isidioclada has isidia but the rhizines are much branched, not simple or bifurcate, than those of the fossil. Thus, the Parmelia ambra fossil was used to calibrate the Parmelia s. s. crown node in the starting tree.
The Alectoria fossil from the Baltic amber [49] is morphologically related to the alectorioid clade (Bryoria, Pseudophebe and Alectoria; [35]). The fossil has abundant apothecia, a character that it shares with Alectoria, while the related Bryoria and Pseudephebe genera rarely have apothecia. Thus this fossil was used to constrain the crown node of Alectoria. Nevertheless, due to the inevitable uncertainty in placement of fossil taxa we assessed the impact of individual fossil calibration on divergence time estimates using alternative analysis: 1) using a single fossil for calibration, either Parmelia (C2 node) or Alectoria (C3 node); and 2) using the Alectoria fossil to calibrate the whole alectorioid clade, including Alectoria, Bryoria and Pseudephebe (C3*).
The divergence time analyses were performed using BEAST v.1.6.1 [93]. We used as starting tree the ML tree obtained with Garli 0.96 [94], made ultrametric using nonparametric rate smoothing (NPRS) implemented in TreeEdit v.10a10 [97] with the divergence between Lecanoromycetes and Chaethothyriomycetidae set at 305 Ma. We constrained the position of Chaethothyriomycetidae as sister clade of Lecanoromycetes based on Schoch et al. [98]. Previously to the analysis, we test that this constraint is not significantly worse than the unconstrained topology, using the Shimodaira-Hasegawa test (SH) [99] and Expected Likelihood Weights test (ELW) [100]. Both tests were run on Tree-Puzzle 5.2 [101].
The final dating analysis was performed with a partitioned BEAST analysis with unlinked substitutions models (GTR+I+G) across the loci, a Birth-Death process tree prior, and a relaxed clock model (uncorrelated lognormal) for each partition. Calibration points were defined as prior distributions: 1) the split of Lecanoromycetes and Chaethothyriomycetidae (C1) was calibrated with a uniform distribution (280–330 Ma). 2) The calibrations points with fossils were considered as minimal ages and calibrated with a lognormal distribution [102]. The Parmelia crown node (C2) at log-normal mean = 2.77, offset = 14, lognormal standard deviation = 0.5. The Alectoria crown node (C3) at lognormal mean = 3.61, offset = 34, lognormal standard deviation = 0.75. The final analysis was run for 10 million generations, with parameter values sampled every 1000 generation. We checked the stationary plateau with Tracer v. 1.4.1 [103]. We discarded 10% of the initial trees as burn in and the consensus tree was calculated using Tree Annotator v 1.6.1 [96]. The results were visualized with FigTree v. 1.3.1 [104]. The substitution rates for each locus were obtained running independent BEAST analyses for each dataset using the same parameters as in the partitioned analysis. Ages and rates were estimated for all the nodes with more than 0.95 of posterior probability both in the BEAST runs and in the previous Bayesian analysis.
Supporting Information
Table S1.
Specimens used in this study with GenBank accession numbers.
https://doi.org/10.1371/journal.pone.0028161.s001
(DOC)
Acknowledgments
We thank Allan Green (Madrid) who kindly facilitated lodging for the visit to Madrid of HTL, and Jack Elix (Australia) for all the help during the PhD of GAP.
Author Contributions
Performed the experiments: GAdP PKD. Analyzed the data: GAdP PC PKD. Contributed reagents/materials/analysis tools: GAdP PC PKD HTL AC. Wrote the paper: GAdP PC PKD HTL AC. Contributed to analyses and discussion: GAdP PC PKD HTL AC.
References
- 1.
Galloway DJ (1979) Biogeographical elements in the New Zealand lichen flora. In: Bramwell D, editor. Plants and Islands. London: Academic Press. pp. 201–224.
- 2. Galloway DJ (1987) Austral lichen genera: some biogeographical problems. Bibl Lichenol 25: 385–399.
- 3. Galloway DJ (1988) Plate tectonics and the distribution of cool temperate Southern Hemisphere macrolichens. Bot J Linn Soc 96: 45–55.
- 4.
Galloway DJ (1991) Phytogeography of Southern hemisphere lichens. In: Nimis PL, CrovelloTJE , editors. Quantitative Approaches to Phytogeography. Dordrecht: Kluwer Academic Publishers. pp. 233–262.
- 5. Jørgensen PM, Sipman HJM (2002) The lichen genus Erioderma in the Southeast Asia. Ann Bot Fenn 39: 201–211.
- 6. Jørgensen PM, van den Boom PPG, Sérusiaux E (2009) Notes on the lichen genus Erioderma in La Réunion. Cryptogamie, Mycologie 30: 263–268.
- 7. Crespo A, Gavilán R, Elix JA, Gutiérrez G (1999) A comparison of morphological, chemical and molecular characters in parmelioid genera. Lichenologist 31: 451–460.
- 8. Grube M, Kroken S (2000) Molecular approaches and the concept of species and species complexes in lichenized fungi. Mycol Res 104: 1284–1294.
- 9. Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, et al. (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 31: 21–32.
- 10. Kroken S, Taylor JW (2001) A gene genealogical approach to recognize phylogenetic species boundaries in the lichenized fungus Letharia. Mycologia 93: 38–53.
- 11. Arguello A, Del Prado R, Cubas P, Crespo A (2007) Parmelina quercina (Parmeliaceae, Lecanorales) includes four phylogenetically supported morphospecies. Biol J Linn Soc 91: 455–467.
- 12. Crespo A, Perez-Ortega S (2009) Cryptic species and species pairs in lichens: A discussion on the relationship between molecular phylogenies and morphological characters. Anales Jard Bot Madrid 66S1: 71–81.
- 13. Elix JA, Corush J, Lumbsch HT (2009) Triterpene chemosyndromes and subtle morphological characters characterise lineages in the Physcia aipolia group in Australia (Ascomycota). Syst Biodivers 7: 479–487.
- 14. Wedin M, Westberg M, Crewe AT, Tehler A, Purvis OW (2009) Species delimitation and evolution of metal bioaccumulation in the lichenized Acarospora smaragdula (Ascomycota, Fungi) complex. Cladistics 25: 161–172.
- 15. Crespo A, Lumbsch HT (2010) Cryptic species in lichen-forming fungi. IMA Fungus 1: 167–170.
- 16. Wirtz N, Printzen C, Lumbsch HT (2011) Using haplotype networks, estimation of gene flow and phenotypic characters to understand species delimitation in fungi of a predominantly Antarctic Usnea group (Ascomycota, Parmeliaceae). Org Divers Evol. in press.
- 17. Crespo A, Ferencova Z, Pérez-Ortega S, Argüello A, Elix JA, et al. (2010a) Austroparmelina, a new Australasian lineage in parmelioid lichens (Parmeliaceae, Ascomycota): a multigene and morphological approach. Syst Biodivers 8: 201–221.
- 18. Crespo A, Kauff F, Divakar PK, Amo G, Arguello A, et al. (2010b) Phylogenetic generic classification of parmelioid lichens (Parmeliaceae, Ascomycota) based on molecular, morphological and chemical evidence. Taxon 59: 1735–1753.
- 19. Divakar PK, Ferencova Z, Del Prado R, Lumbsch HT, Crespo A (2010) Remototrachyna, a new tropical lineage in hypotrachynoid lichens (Parmeliaceae, Ascomycota): a multigene and morphological approach. Am J Bot 97: 579–590.
- 20. Lumbsch HT, Parnmen S, Rangsiruji A, Elix JA (2010) Phenotypic disparity and adaptive radiation in the genus Cladia (Lecanorales, Ascomycota). Australian Syst Bot 23: 239–247.
- 21. Parnmen S, Rangsiruji A, Mongkolsuk P, Boonpragob K, Elix JA, et al. (2010) Morphological disparity in Cladoniaceae: The foliose genus Heterodea evolved from fruticose Cladia species (Lecanorales, lichenized Ascomycota). Taxon 59: 841–849.
- 22. Magallon S, Sanderson MJ (2001) Absolute diversification rates in angiosperm clades. Evolution 55: 1762–1780.
- 23. Bell CD, Soltis DE, Soltis PS (2005) The age of the angiosperms: A molecular timescale without a clock. Evolution 59: 1245–1258.
- 24. Magallon SA, Sanderson MJ (2005) Angiosperm divergence times: The effect of genes, codon positions, and time constraints. Evolution 59: 1653–1670.
- 25. Magallon S, Castillo A (2009) Angiosperm diversification through time. Am J Bot 96: 349–365.
- 26. Lücking R, Huhndorf S, Pfister DH, Plata ER, Lumbsch HT (2009) Fungi evolved right on track. Mycologia 101: 810–822.
- 27. Berbee ML, Taylor JW (1993) Dating the evolutionary radiations of the true fungi. Can J Bot 71: 1114–1127.
- 28.
Berbee ML, Taylor JW (2001) Fungal molecular evolution: gene trees and geologic time. The Mycota. Vol. VII. Part B. Systematics and Evolution. In: Mclaughlin DJ, Mclaughlin EG, Lemkeand PAE, editors. Berlin, Heidelberg, New York: Springer-Verlag. pp. 229–245.
- 29. Heckman DS, Geiser DM, Eidell BR, Stauffer RL, Kardos N, et al. (2001) Molecular evidence for the early colonization of land by fungi and plants. Science 293: 1129–1133.
- 30. Padovan ACB, Sanson GFO, Brunstein A, Briones MRS (2005) Fungi evolution revisited: Application of the penalized likelihood method to a Bayesian fungal phylogeny provides a new perspective on phylogenetic relationships and divergence dates of Ascomycota groups. J Mol Evol 60: 726–735.
- 31. Taylor JW, Berbee ML (2006) Dating divergences in the Fungal Tree of Life: review and new analyses. Mycologia 98: 838–849.
- 32. Berbee ML, Taylor JW (2010) Dating the molecular clock in fungi - how close are we? Fungal Biol Rev 24: 1–16.
- 33. Elix JA (1993) Progress in the generic delimitation of Parmelia sensu lato lichens (Ascomycotina: Parmeliaceae) and a synoptic key to the Parmeliaceae. Bryologist 96: 359–383.
- 34. Wedin M, Döring H, Mattsson JE (1999) A multi-gene study of the phylogenetic relationships of the Parmeliaceae. Mycol Res 103: 1185–1192.
- 35. Crespo A, Lumbsch HT, Mattsson JE, Blanco O, Divakar PK, et al. (2007) Testing morphology-based hypotheses of phylogenetic relationships in Parmeliaceae (Ascomycota) using three ribosomal markers and the nuclear RPB1 gene. Mol Phylogenet Evol 44: 812–824.
- 36. Hawksworth DL, Blanco O, Divakar PK, Ahti T, Crespo A (2008) A first checklist of parmelioid and similar lichens in Europe and adjacent territories, adopting revised generic circumscriptions and with indications of species distribution. Lichenologist 40: 1–21.
- 37. Persoh D, Rambold G (2002) Phacopsis - a lichenicolous genus of the family Parmeliaceae. Mycol Prog 1: 43–55.
- 38. DePriest PT (1999) Development of Mason E. Hale's list of epithets in the parmelioid genera (lichen-forming ascomycotina): A bibliographic review. Bryologist 102: 442–461.
- 39. Hale BW, DePriest PT (1999) Mason E. Hale's list of epithets in the parmelioid genera. Bryologist 102: 462–544.
- 40. Mattsson J-E, Wedin M (1999) A re-assessment of the family Alectoriaceae. Lichenologist 31: 431–440.
- 41. Blanco O, Crespo A, Divakar PK, Esslinger TL, Hawksworth DL, et al. (2004a) Melanelixia and Melanohalea, two new genera segregated from Melanelia (Parmeliaceae) based on molecular and morphological data. Mycol Res 108: 873–884.
- 42. Blanco O, Crespo A, Elix JA, Hawksworth DL, Lumbsch HT (2004b) A molecular phylogeny and a new classification of parmelioid lichens containing Xanthoparmelia-type lichenan (Ascomycota: Lecanorales). Taxon 53: 959–975.
- 43. Thell A, Feuerer T, Kärnefelt I, Myllys L, Stenroos S (2004) Monophyletic groups within the Parmeliaceae identified by ITS rDNA, b-tubulin and GAPDH sequences. Mycol Prog 3: 297–314.
- 44. Blanco O, Crespo A, Divakar PK, Elix JA, Lumbsch HT (2005) Molecular phylogeny of parmotremoid lichens (Ascomycota, Parmeliaceae). Mycologia 97: 150–159.
- 45. Blanco O, Crespo A, Ree RH, Lumbsch HT (2006) Major clades of parmeliold lichens (Parmeliaceae, Ascomycota) and the evolution of their morphological .and chemical diversity. Mol Phylogenet Evol 39: 52–69.
- 46. Arup U, Ekman S, Grube M, Mattsson JE, Wedin M (2007) The sister group relation of Parmeliaceae (Lecanorales, Ascomycota). Mycologia 99: 42–49.
- 47. Divakar PK, Crespo A, Blanco O, Lumbsch HT (2006) Phylogenetic significance of morphological characters in the tropical Hypotrachyna clade of parmelioid lichens (Parmeliaceae, Ascomycota). Mol Phylogenet Evol 40: 448–458.
- 48. Lumbsch HT, Hipp AL, Divakar PK, Blanco O, Crespo A (2008) Accelerated evolutionary rates in tropical and oceanic parmelioid lichens (Ascomycota). BMC Evol Biol 8: 257.
- 49. Mägdefrau K (1957) Flechten und Moose in baltischen Bernstein. Ber Deut Bot Ges 70: 433–435.
- 50. Poinar GO, Peterson EB Jr, Platt JL (2000) Fossil Parmelia in new world amber. Lichenologist 32: 263–269.
- 51. Rikkinen J, Poinar GO (2002) Fossilised Anzia (Lecanorales, lichen-forming Ascomycota) from European Tertiary amber. Mycol Res 106: 984–990.
- 52. Crespo A, Blanco O, Hawksworth DL (2001) The potential of mitochondrial DNA for establishing phylogeny and stabilising generic concepts in the parmelioid lichens. Taxon 50: 807–819.
- 53. Lutzoni F, Pagel M, Reeb V (2001) Major fungal lineages are derived from lichen symbiotic ancestors. Nature 411: 937–940.
- 54. Wedin M, Wiklund E, Crewe A, Döring H, Ekman S, et al. (2005) Phylogenetic relationships of Lecanoromycetes (Ascomycota) as revealed by analyses of mtSSU and nLSU rDNA sequence data. Mycol Res 109: 159–172.
- 55. Thell A, Stenroos S, Feuerer T, Kärnefelt I, Myllys L, et al. (2002) Phylogeny of cetrarioid lichens (Parmeliaceae) inferred from ITS and ß-tubulin sequences, morphology, anatomy and secondary chemistry. Mycological Progress 1: 335–354.
- 56. Thell A, Högnabba F, Elix JA, Feuerer T, Kärnefelt I, et al. (2009b) Phylogeny of the cetrarioid core (Parmeliaceae) based on five genetic markers. Lichenologist 41: 489–511.
- 57. Takamatsu S, Matsuda A (2004) Estimation of molecular clocks for ITS and 28S rDNA in Erysiphales. Mycoscience 45: 340–344.
- 58. Otalora MAG, Martinez I, Aragon G, Molina MC (2010) Phylogeography and divergence date estimates of a lichen species complex with a disjunct distribution pattern. Am J Bot 97: 216–223.
- 59. Sérusiaux E, Villarreal JC, Wheeler T, Goffinet B (2011) Recent origin, active speciation and dispersal for the lichen genus Nephroma (Peltigerales) in Macaronesia. J Biogeogr 38: 1138–1151.
- 60. Divakar PK, Upreti DK (2005) A new species in Melanohalea (Parmeliaceae, Ascomycotina) and new lichen records from India. Lichenologist 37: 511–517.
- 61. Thell A, Elix JA, Søchting U (2009a) Xanthoparmelia lineola s. l. in Australia and North America. Bibl Lichenol 99: 393–404.
- 62. Amo De Paz G, Lumbsch HT, Cubas P, Elix JA, Crespo A (2010a) The genus Karoowia (Parmeliaceae, Ascomycota) includes unrelated clades nested within Xanthoparmelia. Aust Syst Bot 23: 173–184.
- 63. Amo De Paz G, Lumbsch HT, Cubas P, Elix JA, Crespo A (2010b) The morphologically deviating genera Omphalodiella and Placoparmelia belong to Xanthoparmelia (Parmeliaceae). Bryologist 113: 376–386.
- 64. Hodkinson BP, Lendemer JC (2011) Molecular analyses reveal semi-cryptic species in Xanthoparmelia tasmanica. Bibliotheca Lichenologica 106: 108–119.
- 65. Leavitt SD, Johnson L, Clair LL St (2011) Species delimitation and evolution in morphologically and chemically diverse communities of the lichen-forming genus Xanthoparmelia (Parmeliaceae, Ascomycota) in western North America. Am J Bot 98: 175–188.
- 66. Galloway DJ, Quilhot W (1998) Checklist of Chilean lichen-forming and lichenicolous fungi. Gayana Botánica (Chile) 55: 111–185.
- 67. Givnish TJ, Renner SS (2004) Tropical intercontinental disjunctions: Gondwana breakup, immigration from the boreotropics, and transoceanic dispersal. Int J Plant Sci (Chicago) 165: S1–S6.
- 68. Cook LG, Crisp MD (2005) Not so ancient: the extant crown group of Nothofagus represents a post-Gondwanan radiation. Proc R Soc B 272: 2535–2544.
- 69. Knapp M, Stäckler K, Havell D, Delsuc FDR, Sebastiani F, et al. (2005) Relaxed Molecular Clock Provides Evidence for Long-Distance Dispersal of Nothofagus (Southern Beech). PLoS Biol 3: e14.
- 70. Heinrichs J, Lindner M, Groth H, Hentschel J, Feldberg K, et al. (2006) Goodbye or welcome Gondwana? insights into the phylogenetic biogeography of the leafy liverwort Plagiochila with a description of Proskauera gen. nov (Plagiochilaceae, Jungermanniales). Plant Sys Evol 258: 227–250.
- 71. Meudt HM, Simpson BB (2006) The biogeography of the austral, subalpine genus Ourisia (Plantaginaceae) based on molecular phylogenetic evidence: South American origin and dispersal to New Zealand and Tasmania. Biol J Linn Soc 87: 479–513.
- 72. Perrie L, Brownsey P (2007) Molecular evidence for long-distance dispersal in the New Zealand pteridophyte flora. J Biogeogr 34: 2028–2038.
- 73. Hosaka K, Castellano MA, Spatafora JW (2008) Biogeography of Hysterangiales (Phallomycetidae, Basidiomycota). Mycol Res 112: 448–462.
- 74. Moncalvo JM, Buchanan PK (2008) Molecular evidence for long distance dispersal across the Southern Hemisphere in the Ganoderma applanatum-australe species complex (Basidiomycota). Mycol Res 112: 425–436.
- 75. Michalak I, Zhang LB, Renner SS (2010) Trans-Atlantic, trans-Pacific and trans-Indian Ocean dispersal in the small Gondwanan Laurales family Hernandiaceae. J Biogeogr 37: 1214–1226.
- 76. Renner SS, Strijk JS, Strasberg D, Thebaud C (2010) Biogeography of the Monimiaceae (Laurales): a role for East Gondwana and long-distance dispersal, but not West Gondwana. J Biogeogr 37: 1227–1238.
- 77.
Nash TH III, Elix JA (2002) Myelochroa. In: Nash TH III, Ryan BD, Gries C, Bungartz F, editors. Lichen Flora of the greater Sonoran Desert Region. Tempe, vol 1 Arizona. Arizona State University. pp. 288–289.
- 78.
Wolfe JA (1985) Distribution of major vegetational types during the Tertiary. In: Sundquist ET, Broecker WS, editors. The carbon cycle and atmospheric CO2: Natural variations archean to present. WashingtonDC: American Geophysical Union. pp. 357–375.
- 79. Utescher T, Mosbrugger V (2007) Eocene vegetation patterns reconstructed from plant diversity - A global perspective. Palaeogeogr Palaeocl 247: 243–271.
- 80. Zachos JC, Dickens GR, Zeebe RE (2008) An early Cenozoic perspective on greenhouse warming and carbon-cycle dynamics. Nature 451: 279–283.
- 81. Rea DK, Zachos JC, Owen RM, Gingerich PD (1990) Global change at the Paleocene-Eocene boundary: climatic and evolutionary consequences of tectonic events. Palaeogeogr Palaeocl 79: 117–128.
- 82.
Sloan LC, Barron EJ (1992) Paleogene climatic evolution: a climate model investigation of the influence of continental elevation and sea-surface temperature upon continental climate. In: Prothero DR, Berggren WA, editors. Eocene-Oligocene climate and biotic evolution. New York: Princeton University Press. pp. 202–217.
- 83. Lumbsch HT, Schmitt I, Lücking R, Wiklund E, Wedin M (2007a) The phylogenetic placement of Ostropales within Lecanoromycetes (Ascomycota) revisited. Mycol Res 111: 257–267.
- 84. Lumbsch HT, Schmitt I, Mangold A, Wedin M (2007b) Ascus types are phylogenetically misleading in Trapeliaceae and Agyriaceae (Ostropomycetidae, Ascomycota). Mycol Res 111: 1133–1141.
- 85. Miadlikowska J, Kauff F, Hofstetter V, Fraker E, Grube M, et al. (2006) New insights into classification and evolution of the Lecanoromycetes (Pezizomycotina, Ascomycota) from phylogenetic analyses of three ribosomal RNA- and two protein-coding genes. Mycologia 98: 1088–1103.
- 86. Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, et al. (2007) A higher-level phylogenetic classification of the Fungi. Mycol Res 111: 509–547.
- 87. Pybus OG, Harvey PH (2000) Testing macro-evolutionary models using incomplete molecular phylogenies. Proc R Soc B Biol Sci 267: 2267–2272.
- 88. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
- 89. Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17: 540–552.
- 90. Talavera G, Castresana J (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol 56: 564–577.
- 91. Posada D (2008) jModelTest: Phylogenetic Model Averaging. Molecular Biology and Evolution 25: 1253–1256.
- 92. Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52: 696–704.
- 93. Drummond AJ, Rambaut A (2007) Beast: Bayesian evoluionary analysis by sampling trees. BMC Evol Biol 7: 214.
- 94.
Zwickl DJ (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. dissertation. The University of Texas at Austin, Austin.
- 95. Huelsenbeck JP, Ronquist F (2001) Mrbayes: Bayesian Inference of Phylogenetic Trees. Bioinformatics 17: 754–755.
- 96. Kurokawa S (1994) : Japanese species of Parmelia Ach. (sens. str.), Parmeliaceae (2). - J Jpn Bot 69: 121–126.
- 97.
Rambaut A (2007) TreeEdit v.1.0a10. http://tree.bio.ed.ac.uk/software/treeedit/.
- 98. Schoch CL, Sung GH, Lopez-Giraldez F, Townsend JP, Miadlikowska J, et al. (2009) The Ascomycota Tree of Life: A Phylum-wide Phylogeny Clarifies the Origin and Evolution of Fundamental Reproductive and Ecological Traits. Syst Biol 58: 224–239.
- 99. Shimodaira H, Hasegawa M (2001) CONSEL: for assessing the confidence of phylogenetic tree selection. Bioinformatics 17: 1246–1247.
- 100. Strimmer K, Rambaut A (2002) Inferring confidence sets of possibly misspecified gene trees. Proc R Soc B Biol Sci 269: 137–142.
- 101. Schmidt HA, Strimmer K, Vingron M, Von Haeseler A (2002) TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics 18: 502–504.
- 102. Ho SYW, Phillips MJ (2009) Accounting for calibration uncertainty in phylogenetic estimation of evolutionary divergence times. Syst Biol 58: 367–380.
- 103.
Rambaut A, Drummond AJ (2007) Tracer v.1.4.1. In, http://tree.bio.ed.ac.uk/software/tracer/.
- 104.
Rambaut A (2009) FigTree 1.2.2. http://tree.bio.ed.ac.uk/software/figtree/.
- 105.
Nimis PL (1993) The Lichens of Italy. An annotated catalogue. Monografie XII. Torino: Museo Regionale di Scienze Naturali. 897 p.
- 106. Sanmartín I, Ronquist F (2004) Southern Hemisphere biogeography inferred by event-based models: plant versus animal patterns. Syst Biol 53: 216–243.
- 107. Zachos J, Pagani M, Sloan L, Thomas E, Billups K (2001) Trends, rhythms, and aberrations in global climate 65 Ma to present. Science 292: 686–693.