Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Deletion of L-Selectin Increases Atherosclerosis Development in ApoE−/− Mice

  • Izabela Rozenberg,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland

  • Susanna H. M. Sluka,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland

  • Pavani Mocharla,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland

  • Anders Hallenberg,

    Affiliation Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden

  • Pierre Rotzius,

    Affiliation Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden

  • Jan Borén,

    Affiliation Wallenberg Laboratory, Sahlgrenska Academy at Göteborg University, Goteborg, Sweden

  • Nicolle Kränkel,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland

  • Ulf Landmesser,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland, Cardiology, Cardiovascular Center, University Hospital Zurich, Zurich, Switzerland

  • Lubor Borsig,

    Affiliations Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland, Institute of Physiology, University of Zürich, Zürich, Switzerland

  • Thomas F. Lüscher,

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland, Cardiology, Cardiovascular Center, University Hospital Zurich, Zurich, Switzerland

  • Einar E. Eriksson,

    Affiliations Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden, Department of Molecular Medicine and Surgery, Karolinska Institute, Center for Molecular Medicine, Karolinska Hospital, Stockholm, Sweden

  • Felix C. Tanner

    felix.tanner@access.uzh.ch

    Affiliations Cardiovascular Research, Institute of Physiology, University of Zurich, Zurich, Switzerland, Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland, Cardiology, Cardiovascular Center, University Hospital Zurich, Zurich, Switzerland

Abstract

Atherosclerosis is an inflammatory disease characterized by accumulation of leukocytes in the arterial intima. Members of the selectin family of adhesion molecules are important mediators of leukocyte extravasation. However, it is unclear whether L-selectin (L-sel) is involved in the pathogenesis of atherosclerosis. In the present study, mice deficient in L-selectin (L-sel/) animals were crossed with mice lacking Apolipoprotein E (ApoE/). The development of atherosclerosis was analyzed in double-knockout ApoE/L-sel (ApoE/ L-sel/) mice and the corresponding ApoE/ controls fed either a normal or a high cholesterol diet (HCD). After 6 weeks of HCD, aortic lesions were increased two-fold in ApoE/ L-sel/ mice as compared to ApoE/ controls (2.46%±0.54% vs 1.28%±0.24% of total aortic area; p<0.05). Formation of atherosclerotic lesions was also enhanced in 6-month-old ApoE/ L-sel/ animals fed a normal diet (10.45%±2.58% vs 1.87%±0.37%; p<0.05). In contrast, after 12 weeks of HCD, there was no difference in atheroma formation between ApoE/ L-sel/ and ApoE/ mice. Serum cholesterol levels remained unchanged by L-sel deletion. Atherosclerotic plaques did not exhibit any differences in cellular composition assessed by immunohistochemistry for CD68, CD3, CD4, and CD8 in ApoE/ L-sel/ as compared to ApoE/ mice. Leukocyte rolling on lesions in the aorta was similar in ApoE/ L-sel/ and ApoE/ animals. ApoE/ L-sel/ mice exhibited reduced size and cellularity of peripheral lymph nodes, increased size of spleen, and increased number of peripheral lymphocytes as compared to ApoE/ controls. These data indicate that L-sel does not promote atherosclerotic lesion formation and suggest that it rather protects from early atherosclerosis.

Introduction

Endothelial activation and subsequent accumulation of leukocytes is a key event in early atherosclerosis [1]. The selectin family of adhesion molecules mediates initial rolling and tethering of inflammatory cells at sites of activated endothelium [2], [3], [4], [5], [6]. The family consists of the three closely homologous glycoproteins E-selectin (E-sel), P-selectin (P-sel), and L-selectin (L-sel), that all bind glycoproteins and glycolipids bearing sialyl Lewis X (sLeX) in a calcium-dependent manner [7], [8]. Upon stimulation, E-sel is expressed on endothelial cells, while P-sel is expressed in both endothelial cells and platelets. L-sel, on the other hand, is constitutively expressed on the majority of leukocytes [6].

L-sel exhibits adhesive as well as signaling functions [9], [10] and is particularly important for lymphocyte homing to secondary lymphoid organs [5], [11]. Indeed, animals lacking L-sel display an altered size of secondary lymphoid tissues and increased numbers of peripheral lymphocytes [11], [12], [13]. Moreover, L-sel deficient mice show reduced leukocyte rolling along cytokine-stimulated endothelium in vivo. This is well documented in venules in the microcirculation and primarily depends on a lack of L-sel-mediated interactions between leukocytes regulating capture of cells from the free flow [14], [15]. Indeed, whether functional L-sel ligand activity is regularly upregulated on inflamed endothelium is still under debate [16].

Since the selectins are known to regulate leukocyte recruitment in inflammation, they are interesting candidates to study in the context of atherogenesis. Indeed, mice deficient in E- and P-sel display attenuated development of atherosclerosis [2], [17]. Moreover, lymphocyte recruitment to the aortic wall during atherosclerosis development is partially L-sel dependent [18]. However, there are no in vivo reports addressing the impact of L-sel for the development of atherosclerotic lesions in vivo. In this study, L-selectin deficient (L-sel/) mice were crossed with Apolipoprotein E deficient mice (ApoE/) to investigate the relevance of L-sel on both early and advanced stages of atherosclerosis.

Results

L-selectin attenuates early, but not advanced atherosclerosis

The development of atherosclerosis was monitored in descending aortas of mice with or without L-sel. In 12 week old ApoE/ L-sel/ animals fed a HCD for 6 weeks, the percentage of the aorta occupied by atherosclerotic plaques was two fold higher than in age- and diet-matched ApoE/ controls (2.46%±0.54% vs 1.13%±0.19%, respectively; p<0.05; Fig. 1A). The effect of L-sel deletion was even more pronounced in 6 month old animals fed a normal diet. Under these conditions, ApoE/ L-sel/ mice had 10.45±2.58% of the descending aorta covered by plaques as compared to 1.87±0.37% in ApoE/ controls (p<0.05; Fig. 1B). In contrast, the atherosclerotic burden in 18 week old ApoE/ L-sel/ animals fed a HCD for 12 weeks (11.80±1.86%) was similar to that of ApoE/ controls (13.89±2.06%; p = n.s.; Fig. 1C). There was no difference in plasma cholesterol levels between double knockout and control mice in any of the groups (p = n.s.; Table S1). Expression of E-sel did not differ in ApoE/ controls and ApoE/ L-sel/ animals during atherosclerotic lesion formation (p = n.s.; Fig. S1A). P-sel expression was significantly increased in arteries of ApoE/ mice after 6 weeks of HCD compared to ApoE/ L-Sel/ mice. (p<0.05; Fig. S1B) Vascular smooth muscle cell accumulation was similar in the two groups (p = n.s.; Fig. S1D). Staining for collagen exhibited a minor increase in plaques from ApoE/ L-sel/ animals as compared to ApoE/ controls (p<0.05; Fig. S1C).

thumbnail
Figure 1. L-sel modulates atherosclerosis development.

Representative images of aortas stained with Oil-Red-O (ORO). Atherosclerotic lesion area is expressed as percentage of total aortic area. Compared to ApoE/ controls, there is an increased atherosclerotic burden in A) 12 week old ApoE/ L-sel/ animals after 6 weeks of HCD (n = 11–15; *p<0.05); and B) 6 month old ApoE/ L-sel/ animals after 6 months of a normal diet (n = 5–9; *p<0.05), but not C) 18 week old ApoE/ L-sel/ animals after 12 weeks of HCD (n = 10–15; p = n.s.).

https://doi.org/10.1371/journal.pone.0021675.g001

L-selectin does not influence leukocyte capture and rolling in atherosclerosis

Leukocyte capture and rolling were assessed using intravital microscopy. There was no difference between ApoE/ and ApoE/ L-sel/ animals in primary leukocyte capture directly to the endothelium from the free flow (5.4±1.3 cells vs 5.5±1.3 cells, respectively; p = n.s.; Fig. 2A). Secondary capture mediated by interactions between leukocytes was low in ApoE/ as well as in ApoE/ L-sel/ mice (1.28±0.92 cells vs 0.17±0.14 cells, respectively; p = n.s.; Fig. 2B). Correspondingly, there was no difference in the total number of cells rolling along the aortic endothelium in ApoE/ controls and ApoE/ L-sel/ mice (p = n.s.; Fig. 2C). Total capture correlated with the number of rolling cells (Fig. 2D).

thumbnail
Figure 2. L-sel does not affect leukocyte capture and rolling in atherosclerosis.

There was no difference between ApoE/ and ApoE/ L-sel/ animals in either A) primary or B) secondary leukocyte capture (n = 7; p = n.s.). C) Leukocyte rolling in atherosclerotic aorta was not affected by the presence or absence of L-sel (n = 7; p = ns). D) Total leukocyte capture was proportional to leukocyte rolling flux (R = 0.8).

https://doi.org/10.1371/journal.pone.0021675.g002

L-selectin does not influence leukocyte accumulation in atherosclerotic plaques

Immunohistochemistry on the aortic root was performed to monitor the composition of atherosclerotic plaques following L-sel deletion. Macrophages were visualized with anti-CD68 antibody, lymphocytes with anti-CD3 antibody, T-helper and T-cytotoxic cells with anti-CD4 and anti-CD8 antibodies, respectively. L-sel deletion did not result in an altered CD68 positive area in either early (71.10%±1.36% vs 74.44%±2.90%; p = n.s.; Fig. 3A) or advanced atherosclerosis (48.20%±4.13% vs 39.62%±1.99%; p = n.s.; Fig. 3A). Similarly, there was no difference between ApoE/ and ApoE/ L-sel/ mice in the number of CD3 (p = n.s.; Fig. 3B), CD4 (p = n.s.; Fig. 3C), and CD8 positive cells (p = n.s.; Fig. 3D) after 6 and 12 weeks of HCD. Increased duration of HCD resulted in a decreased plaque area occupied by macrophages in both ApoE/ and ApoE/ L-sel/ mice (p<0.05; Fig. 3A). In contrast, no significant difference in T-cell accumulation was observed after 6 and 12 weeks of HCD (p = n.s.). Cytokine expression was similar in aortas of ApoE/ L-sel/ mice as compared to ApoE/ controls after 6 and 12 weeks of HCD (p = ns; Table 1). In contrast, the animals exhibited enhanced cytokine expression after 12 as compared to 6 weeks of HCD irrespective of the genotype (p<0.05; Table 1). The majority of circulating cytokines exhibited similar plasma levels in ApoE/ and ApoE/ L-sel/ mice after 6 weeks of HCD. Interestingly, the level of the chemotactic cytokine MCP-1 was four fold elevated in plasma of ApoE/ L-sel/ mice (p<0.05; Table 2).

thumbnail
Figure 3. L-sel does not affect leucocyte accumulation in atherosclerotic plaques.

Immunohistochemical analysis of A) macrophages (CD68+), B) T-lymphocytes (CD3+), C) T helper cells (CD4+), and D) T-cytotoxic cells (CD8+) in aortic arch after 6 and 12 weeks of HCD (n = 5–13; p = n.s.).

https://doi.org/10.1371/journal.pone.0021675.g003

thumbnail
Table 1. mRNA expression (normalized to S12 expression) of different cytokines in atherosclerotic plaques is not affected by L-sel after 6 and 12 weeks of HCD.

https://doi.org/10.1371/journal.pone.0021675.t001

thumbnail
Table 2. Plasma MCP-1 is elevated in ApoE/ L-sel/ mice compared to ApoE/ mice after 6 weeks of HCD, while the other plasma cytokines are not affected.

https://doi.org/10.1371/journal.pone.0021675.t002

Increased number of circulating lymphocytes in L-sel/ mice

ApoE/ L-sel/ animals exhibited a 1.4 fold and 1.6 fold increased number of blood lymphocytes as compared to ApoE/ controls after 6 and 12 weeks of HCD, respectively (p<0.05; Fig. 4A). Consistent with this observation, there was an increased number of CD8+ and CD19+ cells in ApoE/ L-sel/ mice irrespectively of the duration of HCD (p<0.05; Fig. 4B and C). Moreover, there was tendency towards an increased number of CD4+ cells in ApoE/ L-sel/ mice after 6 weeks of HCD (p = 0.24), which was significant after 12 weeks of this diet (p<0.05; Fig. 4D). The increased number of circulating lymphocytes in ApoE/ L-sel/ mice was associated with an increased number of naive T helper cells (CD4+CD44; p<0.05; Fig. 4E) after 6 and 12 weeks of HCD. The number of activated T helper cells (CD4+CD44+) did not differ after 6 weeks, but was lower in ApoE/ L-sel/ as compared to ApoE/ mice after 12 weeks of HCD (p<0.05; Fig. 4F). No significant difference in the circulating leukocyte profile was observed after 6 and 12 weeks of HCD in any of the genotypes.

thumbnail
Figure 4. L-sel decreases the number of circulating lymphocytes.

ApoE/ L-sel/ animals exhibit increased number of A) total blood lymphocytes (n = 5–8; *p<0.05), B) T-cytotoxic cells (CD8+; n = 6–7; **p<0.01, #p<0.05), C) B lymphocytes (CD19+; n = 5–7; *p<0.05, #p<0.05), D) total T-helper cells (CD4+; n = 6–7; #p<0.0), E) naive T-helper cells (CD4+CD44; n = 5–7; *p<0.05; ##p<0.0001) after 6 and 12 weeks of HCD, respectively. F) The number of activated T-cells (CD4+CD44+) remained unchanged after 6 weeks of HCD (n = 5–7; p = n.s.) and decreased after 12 weeks of HCD (n = 5–7; *p<0.05) as compared to control.

https://doi.org/10.1371/journal.pone.0021675.g004

A significant reduction in size (p<0.05; Fig. S2A) and cellularity (p<0.05; Fig. S2B) of peripheral lymph nodes was observed in ApoE/ L-sel/ as compared to ApoE/ mice after 6 and 12 weeks of HCD. In contrast, the spleen was 30% larger in L-sel deficient mice at both time-points (p<0.05; Fig. S3A). The increased spleen size was not associated with an altered cellularity, cell composition (p = n.s.; Fig. S3B and C) or an altered cytokine expression (p = n.s.; data not shown).

Materials and Methods

Mice

The development of atherosclerosis in ApoE/ L-sel/ mice was studied by two independent experiments. In the first set, L-sel/ mice backcrossed to C57Bl/6 background for 9 generations were used. In the other set, L-sel/ mice were purchased from Jackson Lab and backcrossed to C57Bl/6 background for 5 generations. L-sel/ mice were cross-bred with ApoE/ mice to generate ApoE/ L-sel/ and littermate ApoE/ controls. In the first set, 6 week old males were fed a HCD (Clinton-Cybulski diet, 1.25% cholesterol, Research Diets #D12108) for 6 or 12 weeks. In the other set, male ApoE/ L-sel/ animals and controls were fed a normal diet for 6 months before analysis. All animal experiments were approved by the appropriate authorities.

Quantification of atherosclerosis development

Under anesthesia, blood was collected through right ventricular puncture. Mice were then perfused with ice-cold saline. Aortas were harvested for RNA isolation, en face, and histological analysis. The thoracic and abdominal part of the aortas were fixed overnight with 4% paraformaldehyde (PFA), washed 3 times with ice-cold PBS, and stained for 3 hours with Oil red-O (Sigma #O9755). Quantification of aortic plaque area was performed using AnalysisFIVE software manually by an investigator blinded to the genotypes.

Plasma triglyceride and cholesterol level

Plasma cholesterol level was determined using Infinity™ Cholesterol (Thermo Electron Corporation Standard #TR13421) and MC Cal (Abbott #1E65-02), plasma triglycerides using Infinity™ Triglycerides (Thermo Electron Corporation Standard #TR22421) and MC Cal (Abbott #1E65-02). The distribution of lipids within the plasma lipoprotein fractions was assessed by fast-performance liquid chromatography (FPLC) gel filtration using a Superose 6 HR 10/30 column (Pharmacia).

Immunohistochemistry

Aortic roots were harvested, mounted in O.C.T. compound (Tissue-Tek #62550-01) and frozen at −20°C. 8 µm-thick slices were fixed with 4% PFA and stained with anti-mouse CD3, CD4 CD8, or CD68 antibodies (Serotec), followed by incubation with alkaline phosphatase–conjugated secondary antibody (Jackson ImmunoResearch). Aortic arches were fixed with 4% formalin and embedded in paraffin. Sections were stained with anti-mouse α-SMA (clone 1A4, SIGMA), E-selectin (abcam), and P-selectin (LSBio). Percentages of stained area were quantified with AnalySIS-FIVE program.

Intravital microscopy

18 week old male mice fed with a HCD for 12 weeks were anesthetized with isofluran. The aorta was prepared as described previously [19]. Briefly, the abdomen was opened by a midline incision and the intestines were retracted. The peritoneum was then dissected to expose the abdominal aorta. The exposed tissue was superfused with a thermostated (37°C) bicarbonate-buffered saline solution. Microscopic observations were made using an intravital microscope (Leitz Biomed) with a water immersion objective (Leitz SW 25×). Epi-illumination fluorescence microscopy (Leitz Ploem-o-pac, filter block M2 illuminated by a cooled infrared filtered lamp (Osram HBO 200W/4)) was started 2 minutes after labeling of circulating leukocytes with an intravenous injection of rhodamine 6G (0.3 mg/ml, 0.67 mg/kg). Images were televised and recorded on videotape using a VNC-703 video camera. Leukocyte rolling flux was determined as the average number of leukocytes rolling within a 10000 µm2 area during 30 seconds within a total observation time of at least 180 seconds. Leukocyte capture was determined as the number of leukocytes that initiated rolling within a 10000 µm2 area during 30 seconds [20]. Leukocyte capture in contact with or 50 µm downstream of rolling or adherent leukocytes were regarded as secondary, all other capture was regarded as primary.

Facs

Blood cells were stained with fluorescently labeled anti-mouse CD4 (PE-conjugated, clone RM4-5), CD8 (PE-Cy7-conjugated, clone H35-17.2), CD19 (PE-Cy7-conjugated, clone 1D3), or CD44 (APC-conjugated, clone IM7) antibodies (Pharmingen) for 30 minutes at 4°C. Erythrocytes were lyzed following staining using commercially available lysis buffer (BD #555899). Data were collected using DIVAII (BD), and FACS analysis was performed using FlowJo software.

Statistical analysis

The results were expressed as mean ± S.E.M. Comparison of means was carried out by Student's t-test or ANOVA in case of multiple comparisons. For each experiment, P<0.05 was accepted as statistically significant.

Discussion

Accumulation of leukocytes in the arterial wall is an important pathogenic event in atherogenesis. It is well documented that the selectin family of adhesion molecules mediates initial attachment of leukocytes to activated endothelium, representing the first step of leukocyte emigration into sites of inflammation [2], [6]. Correspondingly, L-sel may play a role in the migration of leukocytes to atherosclerotic lesions and data have been presented supporting that lymphocyte recruitment during atherosclerosis development is partially L-sel dependent [18]. Thus, we hypothesized that deletion of L-sel might attenuate the development of atherosclerosis due to inhibition of leukocyte rolling and capture. To test this hypothesis, we compared atherogenesis in ApoE/ L-sel/ mice with that of ApoE/ controls [21]. Interestingly, the data show that L-sel does not promote atherosclerotic lesion formation in ApoE/ mice. On the contrary, genetic deficiency in L-sel resulted in a significant increase in lesion formation, at least during early stages of the disease. Indeed, after 6 month of normal diet, atherosclerosis was relatively advanced in the absence of L-sel, while plaque burden was still low in the ApoE/ control group and comparable to the ApoE/ control animals after 6 weeks of HCD. Thus, both feeding protocols induce an early stage of the disease; however, the absence of L-sel results in a strong increase of atherosclerosis. In line with this, there was no decrease in leukocyte rolling between ApoE/ L-sel/ and ApoE/ control mice in the atherosclerotic aorta. These observations indicate that other members of the selectin family are sufficient to maintain leukocyte-endothelium interactions under conditions of L-sel deletion [17], [22], [23]. In line with this interpretation, expression of E-sel and P-sel were not upregulated in the absence of L-sel during atherosclerotic lesion formation. Previous data reveal that the effect of combined deficiency of P- and E-sel has an effect on rolling and recruitment in inflammation which is much stronger than that seen in mice deficient in L-sel [22], [23]. Blockage of P-sel also virtually abolishes interactions between leukocytes and endothelium in the atherosclerotic aorta and inhibition of E-sel stabilizes leukocyte rolling under these conditions [2] supporting that E- and P-sel are key mediators of initial leukocyte attachment in arteries. Combined deficiency in E-sel and P-sel also strongly reduces the formation of atherosclerotic lesions [17]. In contrast, as previously indicated [14], L-sel-dependent secondary capture does not increase rolling on atherosclerotic endothelium. Nonetheless, L-sel increases rolling in venules in the microcirculation [11], [14], [24], which has been shown to be dependent mainly on interactions between leukocytes. Ligands for L-sel are only expressed by endothelium in secondary lymphoid tissues and, under certain circumstances, also by chronically inflamed systemic endothelium [18], [25]. Interestingly, data from a previous study suggested that L-sel dependent accumulation of lymphocytes in arteries occurs almost exclusively from the adventitial side of the vessel suggesting that L-sel influences recruitment from the vasa vasorum [18]. This apparent role of L-sel could be mediated by both direct interactions between leukocytes and endothelium as well as secondary capture interactions. Thus, it is possible that L-sel influences rolling and recruitment in other parts of the vascular wall than in the arterial lumen. A strong argument against this interpretation is that plaques from ApoE/ L-sel/ mice exhibited similar numbers of macrophages and T cells as compared to lesions from ApoE/ controls. Ideally, the cellular composition of the plaque should be examined in the descending aorta, i.e. at that site of the aorta in which significant differences in plaque size were noted. As the atherosclerotic alterations in the descending aorta are focal, it is virtually impossible to cut the descending aorta at the same site and find a plaque of similar size to assess and compare its composition. For this reason, cellular plaque composition was studied in the aortic sinuses, i.e. at a site where plaques of similar size could consistently be detected. It has been observed that even if plaque size is similar at the level of the aortic root, differences in plaque composition can be detected [26].

No difference in cytokine expression in atherosclerotic vessel walls from ApoE/ L-sel/ and ApoE/ mice was detected indicating a similar extent of local inflammation without or with L-sel. The enhanced aortic cytokine levels in animals treated with HCD for 12 weeks as compared to those treated for 6 weeks is consistent with a more advanced stage of atherosclerosis in these mice and did not differ between strains. Hence, alterations in local inflammation do not seem to account for the atheroprotective actions of L-sel. In line with these observations, plasma cytokine levels were similar in ApoE/ L-sel/ and ApoE/ mice. In plasma of ApoE/ L-sel/ mice fed a HCD for 6 weeks the levels of the chemotactic cytokine MCP-1 were elevated compared to ApoE/ mice, which most likely reflects the increased plaque burden in these animals.

Atherosclerosis does not only affect the wall of blood vessels, but also provokes changes at the systemic level [27], [28]. Deletion of L-sel resulted in abnormal systemic leukocyte distribution, which could potentially affect atherosclerosis development [29]. Both size and cellularity of peripheral lymph nodes were decreased in ApoE/ L-sel/ mice as compared to ApoE/ controls, which is consistent with the observation that migration of naive lymphocytes into peripheral lymph nodes is impaired in L-sel deficient mice [11], [13]. Likely due to compensation for this impaired migration into tissues, L-sel deficiency results in increased numbers of circulating lymphocytes. Hence, it is possible that the enhanced atherosclerosis in ApoE/ L-sel/ mice is driven by more abundant circulating proatherogenic cells, in particular because migration of these cells into lesions appears not to be impaired by lack of L-sel [2], [30].

In conclusion, this study demonstrates that absence of L-sel does not inhibit atherosclerosis but rather augments the early stages of atherogenesis. This effect is not associated with reduced leukocyte rolling or accumulation nor with altered cytokine production in atherosclerotic plaques.

Supporting Information

Figure S1.

Histochemical and immunohistochemical stainings of aortic arches from mice fed a HCD for 6 weeks: A) Similar expression of E-sel in ApoE/ and ApoE/ L-sel/ mice. B) Increased expression of P-sel in ApoE/ compared to ApoE/ L-sel/ mice (p<0.05) C) Collagen area is increased in ApoE/ L-Sel/ mice compared to ApoE/ mice (p<0.01) D) Smooth muscle cell area is similar in ApoE/ and ApoE/ L-sel/ mice.

https://doi.org/10.1371/journal.pone.0021675.s001

(TIF)

Figure S2.

Decreased A) size (n = 5–6; **p<0.01; ##p<0.01) and B) cellularity (n = 5–6; *p<0.05; #p<0.05) of peripheral lymph nodes (LN) in mice lacking L-sel after 6 and 12 weeks of HCD.

https://doi.org/10.1371/journal.pone.0021675.s002

(TIF)

Figure S3.

A) Spleen size is increased upon L-sel deletion (n = 6; **p<0.01; #p<0.05). B) Spleen cellularity is not affected by L-sel deletion (n = 5–6; *p = n.s.) after 6 and 12 weeks of HCD. C) Increased size but unchanged cell composition (table; % of leukocytes ± SEM) of spleens from animals after 6 month of normal diet.

https://doi.org/10.1371/journal.pone.0021675.s003

(TIF)

Table S1.

Plasma cholesterol and triglyceride levels (n = 5–12; p = n.s.) after normal diet or 6 and 12 weeks of HCD.

https://doi.org/10.1371/journal.pone.0021675.s004

(DOC)

Acknowledgments

The authors would like to thank Mrs. Silvia Behnke from the Institute of Clinical Pathology (University Hospital Zurich), for her help with α-SMA, E- and P-Selectin immunohistochemical stainings, the lab technicians from the Institute of Veterinary Pathology (University of Zurich), for paraffin sectioning, and Dr. Jens Sobek from the Functional Genomics Center Zurich, for his help with the mouse cytokine array.

Author Contributions

Conceived and designed the experiments: IR SHMS PM NK EEE FCT. Performed the experiments: IR SHMS PM JB NK EEE. Analyzed the data: IR SHMS PM EEE FCT. Contributed reagents/materials/analysis tools: IR PM AH PR JB UL LB TFL. Wrote the paper: IR SHMS EEE FCT.

References

  1. 1. Libby P (2002) Inflammation in atherosclerosis. Nature 420: 868–874.
  2. 2. Eriksson EE, Xie X, Werr J, Thoren P, Lindbom L (2001) Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins. Faseb J 15: 1149–1157.
  3. 3. Kunkel EJ, Jung U, Ley K (1997) TNF-alpha induces selectin-mediated leukocyte rolling in mouse cremaster muscle arterioles. Am J Physiol 272: H1391–1400.
  4. 4. Mayadas TN, Johnson RC, Rayburn H, Hynes RO, Wagner DD (1993) Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell 74: 541–554.
  5. 5. Spertini O, Luscinskas FW, Kansas GS, Munro JM, Griffin JD, et al. (1991) Leukocyte adhesion molecule-1 (LAM-1, L-selectin) interacts with an inducible endothelial cell ligand to support leukocyte adhesion. J Immunol 147: 2565–2573.
  6. 6. Tedder TF, Steeber DA, Chen A, Engel P (1995) The selectins: vascular adhesion molecules. Faseb J 9: 866–873.
  7. 7. Brandley BK, Swiedler SJ, Robbins PW (1990) Carbohydrate ligands of the LEC cell adhesion molecules. Cell 63: 861–863.
  8. 8. Phillips ML, Nudelman E, Gaeta FC, Perez M, Singhal AK, et al. (1990) ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science 250: 1130–1132.
  9. 9. Kilian K, Dernedde J, Mueller EC, Bahr I, Tauber R (2004) The interaction of protein kinase C isozymes alpha, iota, and theta with the cytoplasmic domain of L-selectin is modulated by phosphorylation of the receptor. J Biol Chem 279: 34472–34480.
  10. 10. Giuffre L, Cordey AS, Monai N, Tardy Y, Schapira M, et al. (1997) Monocyte adhesion to activated aortic endothelium: role of L-selectin and heparan sulfate proteoglycans. J Cell Biol 136: 945–956.
  11. 11. Arbones ML, Ord DC, Ley K, Ratech H, Maynard-Curry C, et al. (1994) Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice. Immunity 1: 247–260.
  12. 12. Steeber DA, Green NE, Sato S, Tedder TF (1996) Humoral immune responses in L-selectin-deficient mice. J Immunol 157: 4899–4907.
  13. 13. Tedder TF, Steeber DA, Pizcueta P (1995) L-selectin-deficient mice have impaired leukocyte recruitment into inflammatory sites. J Exp Med 181: 2259–2264.
  14. 14. Eriksson EE, Xie X, Werr J, Thoren P, Lindbom L (2001) Importance of primary capture and L-selectin-dependent secondary capture in leukocyte accumulation in inflammation and atherosclerosis in vivo. J Exp Med 194: 205–218.
  15. 15. Sperandio M, Smith ML, Forlow SB, Olson TS, Xia L, et al. (2003) P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules. J Exp Med 197: 1355–1363.
  16. 16. Eriksson EE (2008) No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84: 93–103.
  17. 17. Dong ZM, Chapman SM, Brown AA, Frenette PS, Hynes RO, et al. (1998) The combined role of P- and E-selectins in atherosclerosis. J Clin Invest 102: 145–152.
  18. 18. Galkina E, Kadl A, Sanders J, Varughese D, Sarembock IJ, et al. (2006) Lymphocyte recruitment into the aortic wall before and during development of atherosclerosis is partially L-selectin dependent. J Exp Med 203: 1273–1282.
  19. 19. Eriksson EE, Werr J, Guo Y, Thoren P, Lindbom L (2000) Direct observations in vivo on the role of endothelial selectins and alpha(4) integrin in cytokine-induced leukocyte-endothelium interactions in the mouse aorta. Circ Res 86: 526–533.
  20. 20. Rotzius P, Thams S, Soehnlein O, Kenne E, Tseng CN, et al. (2010) Distinct infiltration of neutrophils in lesion shoulders in ApoE-/- mice. Am J Pathol 177: 493–500.
  21. 21. Rotzius P, Soehnlein O, Kenne E, Lindbom L, Nystrom K, et al. (2009) ApoE(-/-)/lysozyme M(EGFP/EGFP) mice as a versatile model to study monocyte and neutrophil trafficking in atherosclerosis. Atherosclerosis 202: 111–118.
  22. 22. Jung U, Ley K (1999) Mice lacking two or all three selectins demonstrate overlapping and distinct functions for each selectin. J Immunol 162: 6755–6762.
  23. 23. Rigby S, Dailey MO (2000) Traffic of L-selectin-negative T cells to sites of inflammation. Eur J Immunol 30: 98–107.
  24. 24. Ley K, Gaehtgens P, Fennie C, Singer MS, Lasky LA, et al. (1991) Lectin-like cell adhesion molecule 1 mediates leukocyte rolling in mesenteric venules in vivo. Blood 77: 2553–2555.
  25. 25. Rivera-Nieves J, Burcin TL, Olson TS, Morris MA, McDuffie M, et al. (2006) Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis. J Exp Med 203: 907–917.
  26. 26. Rozenberg I, Sluka SH, Rohrer L, Hofmann J, Becher B, et al. (2010) Histamine H1 receptor promotes atherosclerotic lesion formation by increasing vascular permeability for low-density lipoproteins. Arterioscler Thromb Vasc Biol 30: 923–930.
  27. 27. Mullenix PS, Andersen CA, Starnes BW (2005) Atherosclerosis as inflammation. Ann Vasc Surg 19: 130–138.
  28. 28. Willerson JT (2002) Systemic and local inflammation in patients with unstable atherosclerotic plaques. Prog Cardiovasc Dis 44: 469–478.
  29. 29. Hansson GK, Libby P, Schonbeck U, Yan ZQ (2002) Innate and adaptive immunity in the pathogenesis of atherosclerosis. Circ Res 91: 281–291.
  30. 30. Ramos CL, Huo Y, Jung U, Ghosh S, Manka DR, et al. (1999) Direct demonstration of P-selectin- and VCAM-1-dependent mononuclear cell rolling in early atherosclerotic lesions of apolipoprotein E-deficient mice. Circ Res 84: 1237–1244.