Research Article

Substantial Contribution of Submicroscopical Plasmodium falciparum Gametocyte Carriage to the Infectious Reservoir in an Area of Seasonal Transmission

  • André Lin Ouédraogo mail,

    Affiliations: Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso, Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

  • Teun Bousema,

    Affiliation: Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom

  • Petra Schneider,

    Affiliation: Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, United Kingdom

  • Sake J. de Vlas,

    Affiliation: Department of Public Health, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands

  • Edith Ilboudo-Sanogo,

    Affiliation: Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso

  • Nadine Cuzin-Ouattara,

    Affiliation: Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso

  • Issa Nébié,

    Affiliation: Department of Biomedical Sciences, Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso

  • Will Roeffen,

    Affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

  • Jan Peter Verhave,

    Affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

  • Adrian J. F. Luty,

    Affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

  • Robert Sauerwein

    Affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, The Netherlands

  • Published: December 22, 2009
  • DOI: 10.1371/journal.pone.0008410



Man to mosquito transmission of malaria depends on the presence of the sexual stage parasites, gametocytes, that often circulate at low densities. Gametocyte densities below the microscopical threshold of detection may be sufficient to infect mosquitoes but the importance of submicroscopical gametocyte carriage in different transmission settings is unknown.

Methodology/Principal Findings

Membrane feeding experiments were carried out on 80 children below 14 years of age at the end of the wet season in an area of seasonal malaria transmission in Burkina Faso. Gametocytes were quantified by microscopy and by Pfs25-based quantitative nucleic acid sequence-based amplification assay (QT-NASBA). The children's infectiousness was determined by membrane feeding experiments in which a venous blood sample was offered to locally reared Anopheles mosquitoes. Gametocytes were detected in 30.0% (24/80) of the children by microscopy compared to 91.6% (65/71) by QT-NASBA (p<0.001). We observed a strong association between QT-NASBA gametocyte density and infection rates (p = 0.007). Children with microscopically detectable gametocytes were more likely to be infectious (68.2% compared to 31.7% of carriers of submicroscopical gametocytes, p = 0.001), and on average infected more mosquitoes (13.2% compared to 2.3%, p<0.001). However, because of the high prevalence of submicroscopical gametocyte carriage in the study population, carriers of sub-microscopical gametocytes were responsible for 24.2% of the malaria transmission in this population.


Submicroscopical gametocyte carriage is common in an area of seasonal transmission in Burkina Faso and contributes substantially to the human infectious reservoir. Submicroscopical gametocyte carriage should therefore be considered when implementing interventions that aim to reduce malaria transmission.


The transmission of malaria depends on the presence of mature sexual stage parasites, gametocytes, in the human peripheral blood. Once ingested by a mosquito taking a blood meal, gametocytes develop through different mosquito-specific stages and ultimately result in infection of the mosquito salivary glands with sporozoites. This renders the mosquito infectious to humans. There is growing awareness that targeting gametocytes, either alone or as part of integrated control programmes, is essential for malaria control and elimination efforts [1], [2], [3], [4]. The identification of the human infectious reservoir is therefore important for successful malaria control. Gametocytes frequently occur at low densities, making microscopical detection complicated [5]. In the last decade, molecular tools have become available to detect and quantify gametocytes at densities well below the microscopical threshold, in the order of 0.02–10 gametocytes/µL of blood [4]. Using these techniques, it has become evident that the proportion of gametocyte carriers in the population has been grossly underestimated and that the gametocyte reservoir may be 2–5 fold larger than assumed based on microscopy [6], [7]. Carriers of gametocytes at submicroscopical levels are capable of infecting mosquitoes [7], [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18], [19] although at a lower degree than those with gametocytes detectable microscopically in whom gametocytes are present at higher densities [12], [18].

The importance of submicroscopical gametocyte carriage for malaria epidemiology and malaria control is the subject of some debate. While carriers of gametocytes at submicroscopical densities were concluded to be as important for the human infectious reservoir as carriers of microscopically-detectable gametocytes in areas of perennial transmission in Kenya [18] and Thailand [16], data from the Gambia suggest that submicroscopical gametocyte carriers only form a very small fraction of the infectious reservoir in this area of seasonal transmission [20]. This suggests that the relevance of submicroscopical gametocyte carriage may depend on transmission settings.

Here, we determine the contribution of submicroscopical densities of gametocytes to the human infectious reservoir in an area of seasonal malaria transmission in Burkina Faso.


This study was conducted in September-November 2005 in the village of Laye, 30 km northwest of Ouagadougou, Burkina Faso. The area is characterised by Sudanese savannah with a marked wet season from June to October and an estimated entomological inoculation rate of 300–500 infective bites per person per year [21]. Asexual parasite carriage in the population shows seasonal fluctuations and was recently estimated at 60–90% in children below 15 years of age and 20–50% in adults [22]. Clearance was received by the Ministry of Health of Burkina Faso. Children below 14 years of age were randomly selected from village census lists and written informed consent was obtained from parents/guardians after the purpose of the study was explained. Children were accompanied to the Centre National de Recherche et de Formation sur le Paludisme (CNRFP) 1 to 2 days after the consenting procedure. At CNRFP, children underwent a clinical examination and their axillary temperature was measured. Children were enrolled in membrane feeding experiments regardless of symptoms or the presence of asexual malaria parasites or gametocytes. Venous blood samples (3 mL) were drawn into heparin-containing tubes for membrane feeding and for gametocyte detection both by microscopy and by real-time Pfs25 quantitative nucleic acid sequence based amplification (QT-NASBA). For all membrane-feeding assays, 3 mL venous blood samples were obtained and fed to ~50 locally colony-reared 4–5-day-old female A. gambiae sensu stricto mosquitoes. The mosquito colony was established three years prior to the current experiments. Blood was offered via an artificial membrane attached to a water-jacketed glass feeder maintained at 37°C. After 10–15 min, fully fed mosquitoes were selected and kept on glucose at 29°C [7]. Unfed and partially fed mosquitoes were removed by aspiration and discarded. Mosquito midguts were examined after 7 days for the presence of oocysts following dissection in 2% mercurochrome. A second microscopist confirmed the presence of oocysts in midguts that were scored positive.

Only the total number of oocysts per batch of fed mosquitoes was recorded; not the number of oocysts per individual mosquito.Experiments in which a minimum of 10 mosquitoes were examined on day 7 after feeding were included in the analyses. After membrane feedings, children with fever (axillary temperature ≥37.5°C) and malaria parasites were treated with artemisinin-based combination therapy according to the national guidelines. Individuals for whom infections other than malaria were suspected were accompanied to the nearest health facility for appropriate clinical care. The study protocol was viewed and approved by the Ministry of Health of Burkina Faso on August 8th 2000(Research's Authorization number 2000/3174/MS/SG/DEP).

Microscopical Detection of P. falciparum Parasites

Samples were considered negative if no parasites were detected in 100 fields. Both asexual stage and gametocyte densities were simultaneously assessed by counting against 1000 leucocytes in the thick smear. The lower limit of microscopy for gametocyte quantification was therefore estimated at 8 gametocytes/ µl of blood. Parasite counts were converted to numbers of parasites per µl by assuming a standard count of 8000 leucocytes/µl of blood. Each sample was read independently by two microscopists and the mean density was used. A third reader was involved when the first two readers disagreed about the prevalence of gametocytes or their estimated densities differed ≥30%. In these cases the mean density of the two closest readings was used.

Gametocyte Detection by Real Time Pfs25 QT-NASBA

Gametocyte detection by Pfs25 QT-NASBA was performed as described elsewhere using a NucliSens EasyQ analyser (Bio-Mérieux) [23], [24]. Nucleic acid was extracted from 50-µL blood samples as described by Boom et al.[25]. The first part of the RNA extraction was done in the field following the original guanidinium isothiocyanate (GuSCN) RNA extraction method [25] until the nucleic acids were bound to silica dioxide particles. At this point, samples were stored at −20°C and transferred to the laboratory for completion of the extraction and QT-NASBA analysis. The number of gametocytes was calculated in relation to a standard gametocyte stage V dilution series [26], using the time point of amplification at which the fluorescence detecting target amplicons exceeded the mean fluorescence of three negative controls + 20 standard deviations. The Pfs25 QT-NASBA technique is gametocyte specific and has a detection limit of 20–100 gametocytes/mL[24]. Samples with Pfs25 QT-NASBA gametocyte concentrations <20 gametocytes/mL were considered gametocyte negative.

Sample Size Considerations

Based on a previous study in the area, we expected a gametocyte prevalence of 10–20% by microscopy and 70–80% by Pfs25 QT-NASBA [23]. Including 80 individuals in the membrane feed experiments would allow us to detect a threefold lower infectiousness of submicroscopical carriers compared to microscopical gametocyte carriers [12], [20] when we assumed that 60% of the microscopical gametocyte carriers infected at least one mosquito [18] (Zα = 1.645; Zβ = 0.84).

Data Analysis

Data analyses were performed using SPSS version 16.0 (SPSS Inc., Chicago, IL, USA) and Stata 10 (Statacorp, Texas US). Densities of gametocytes were analysed on a log10-scale. The prevalence of mosquito infection (i.e. whether an individual infected at least one mosquito) and the proportion of infected mosquitoes were used as outcomes of the membrane feeding experiments and were related to Pfs25 QT-NASBA gametocyte density, age and fever in logistic and linear regression models. Individual oocyst densities in mosquitoes were not recorded.


We enrolled 80 children in our study who were aged 2.9–13.6 years. In line with previous studies from the study area, microscopy indicated an asexual parasite prevalence of 82.5% (66/80) and a gametocyte prevalence of 30.0% (24/80; Table 1) [22], [23]. When the Pfs25 QT-NASBA was used for gametocyte detection, 91.6% (65/71) individuals were shown to be carrying gametocytes. There was a strong correlation between gametocyte densities detected by QT-NASBA and microscopy for microscopically gametocyte positive samples (Spearman correlation coefficient = 0.60; p = 0.004). For nine individuals RNA collection failed, i.e. no sample was collected, and therefore no QT-NASBA data were available. Membrane feeds were successful for all 80 individuals but, due to mosquito mortality between the day of feeding and the day of dissection, only a total of 74 membrane feeds had at least 10 mosquitoes dissected and were therefore included in the analyses. The proportion of infected mosquitoes was positively associated with Pfs25 QT-NASBA gametocyte density (Spearman correlation coefficient = 0.34, p = 0.007; Figure 1) and was not influenced by a clinical malaria episode (i.e. fever with a parasite density ≥500 parasites/µL) (p = 0.18) or the presence of fever (p = 0.63).The relation between the proportion of infected mosquitoes and Pfs25 QT-NASBA gametocyte density was best described by the equation Y = 0.0176Ln(X) + 0.0187 (R2 = 0.153).


Figure 1. The relationship between Pfs25 QT-NASBA gametocyte density and the proportion of infected mosquitoes in membrane feeding experiments.

The solid line indicates the best fitted line (Y = 0.0176Ln(X) + 0.0187; R2 = 0.153). The dashed line indicates the estimated microscopic threshold for gametocyte detection, 8 gametocytes/µL, when screening 100 high power fields (i.e. ~1000 white blood cells).


Table 1. Baseline characteristics.


Of those children with microscopically detectable gametocytes, 68.2% (15/22) infected at least one mosquito compared to 31.7% (13/41) of children with submicroscopical gametocyte densities (p = 0.001; Table 2). The proportion of infected mosquitoes was also higher in carriers of microscopically- compared to submicroscopically-detectable gametocytes (p<0.001). Oocyst densities of individual mosquitoes were not recorded, only the total number of oocysts observed in each experiment. Formal statistical testing on oocyst burden could therefore not be done although the total number of oocysts relative to the number of infected mosquitoes was higher for individuals with microscopically-detectable densities of gametocytes. The relative contribution to transmission of microscopical and submicroscopical gametocyte carriage was determined based on their prevalence in the studied population and the proportion of mosquitoes infected by each group. This resulted in a relative contribution to malaria transmission of 24.2% for submicroscopical gametocyte carriage, compared to 75.8% for microscopical gametocyte carriage.


Table 2. Membrane feeding results.



In this study, we observed that submicroscopical gametocyte carriage is common in children in an area of seasonal malaria in Burkina Faso. Although, on average, carriers of gametocytes at submicroscopical densities infected significantly fewer mosquitoes that themselves developed lower oocyst burdens, the contribution to the infectious reservoir of this age group was considerable and estimated at 24%.

There are numerous reports that suggest that some individuals can infect mosquitoes despite the absence of microscopically detectable gametocytes [7], [8], [10], [12], [16], [18], [19]. However, it is unclear (i) how important this phenomenon is in areas of seasonal malaria [20] and (ii) how important submicroscopical gametocyte densities are for malaria transmission in the general, typically asymptomatic, population. Several detailed studies on the infectiousness of carriers of submicroscopical gametocytes have been carried out, but only after chemotherapeutic treatment of symptomatic malaria cases [7], [18], [20]. Because children in those studies all had high densities of asexual parasites in the weeks prior to the mosquito feeding experiments, they were more likely to have gametocytes at the time of the membrane feedings [5]. Although it was previously shown that gametocyte carriage may be common in asymptomatic individuals as well [27], findings from clinical trials cannot be extrapolated to the general population. To the best of our knowledge, the infectious reservoir has never been determined in the general population by means of the combination of molecular gametocyte detection tools with membrane feeding experiments used in the study described here. Although our experiments were restricted to children, our findings provide valuable information about the infectiousness of a cross-section of the population living in an area of seasonal malaria transmission. At the end of the wet season, 90% of the children in our study area carried gametocytes [23], two-thirds of them at densities below the microscopical threshold of detection. The infectiousness of individuals with submicroscopical gametocyte densities was lower than that of children with microscopical gametocyte densities in terms of (i) prevalence of infection, (ii) proportion of infected mosquitoes and (iii) oocyst burden in mosquitoes. These observations are largely in agreement with previous studies [12], [18] although a study in symptomatic children in Kenya reported that submicroscopical gametocyte carriers were as likely as microscopical gametocyte carriers to be infectious and only the average number of infected mosquitoes and the oocyst burden were lower for submicroscopical gametocyte carriers [18]. This difference may be due to the different populations: we enrolled asymptomatic children compared to the Kenyan study where children were sampled 14 days after a clinical malaria episode [18]. We nevertheless consider the current findings biologically more plausible since the chance of a submicroscopical gametocyte carrier being classified as ‘infectious’ (i.e. infecting at least one mosquito) is likely to be lower if submicroscopical gametocyte carriers on average infect a lower proportion of mosquitoes [12], [18]. Contrary to a recently published hypothesis that infection of mosquitoes by submicroscopical gametocytaemia may be rare in areas of seasonal malaria transmission [20], our findings suggest that carriers of submicroscopical gametocyte densities may be common in these circumstances. The relative contribution to transmission per gametocyte carrier may be lower for submicroscopical gametocyte carriers but their relative abundance in a population appears to counterbalance this and makes them important contributors to malaria transmission. To reliably determine the influence of transmission intensity and seasonality on the occurrence and infectiousness of submicroscopical gametocyte densities, a direct comparison is needed where the infectiousness of different populations is assessed at several time-points during the season.

Our study has two limitations: we determined the infectious reservoir at the end of the wet season only and restricted our experiments to children. Seasonal patterns in gametocyte carriage [22] make it impossible to draw conclusions about the importance of submicroscopical gametocyte carriage for malaria transmission at other time-points in the season. For this, a series of membrane feeding experiments are needed throughout the year. We have previously reported that submicroscopical gametocyte carriage is less prevalent in adults in our study area [23]. Our data can therefore not be extrapolated to the whole population. Although we observed a significant correlation between Pfs25 QT-NASBA gametocyte density and mosquito infection rates, it is not possible to reliably estimate the infectiousness of individuals based on gametocyte density data only. Some children with a gametocyte density below 1 gametocyte/µL were able to infect mosquitoes in our study. This is surprising since a blood meal, that is on average 2–3µL, should contain at least one male and one female gametocyte to result in infection. However, the phenomenon has been observed before [18] and may be influenced by the aggregation of gametocytes that favours the encounter of males and females [28]. Alternatively, we cannot rule out that artefacts resulting from RNA degradation have resulted in unrealistically low estimates of gametocyte densities in occasional samples. We also observed that some carriers of gametocytes at high density were unable to infect mosquitoes [8], [12], [18]. This could be partly due to transmission reducing immune responses [8], [29]. These immune responses may be inversely related to age [18], [29]. Further studies should therefore be conducted at different time-points, include all age groups and preferably incorporate transmission reducing immune responses and sexing of gametocytes to further elucidate the detailed processes that determine the composition of the human infectious reservoir of malaria in a given transmission setting.


We are grateful to all the inhabitants of the study village for their co-operation, the Ministry of health of Burkina Faso and the staff of CNRFP for their assistance in the implementation of this study. We thank Marcel de Kruijf for his assistance in the QT-NASBA analyses.

Author Contributions

Conceived and designed the experiments: ALO SJdV EIS NCO IN JPV RWS. Performed the experiments: ALO PS WR. Analyzed the data: ALO TB PS SJdV AJFL. Wrote the paper: ALO TB AJFL RWS.


  1. 1. Carter R, Mendis KN, Miller LH, Molineaux L, Saul A (2000) Malaria transmission-blocking vaccines–how can their development be supported? NatMed 6: 241–244.
  2. 2. Sauerwein RW (2007) Malaria transmission-blocking vaccines: the bonus of effective malaria control. Microbes Infect 9: 792–795.
  3. 3. White NJ (2008) The role of anti-malarial drugs in eliminating malaria. Malar J 7: Suppl 1S8.
  4. 4. Babiker HA, Schneider P, Reece SE (2008) Gametocytes: insights gained during a decade of molecular monitoring. Trends Parasitol 24: 525–530.
  5. 5. Drakeley C, Sutherland C, Bousema JT, Sauerwein RW, Targett GA (2006) The epidemiology of Plasmodium falciparum gametocytes: weapons of mass dispersion. Trends Parasitol 22: 424–430.
  6. 6. Shekalaghe SA, Bousema JT, Kunei KK, Lushino P, Masokoto A, et al. (2007) Submicroscopic Plasmodium falciparum gametocyte carriage is common in an area of low and seasonal transmission in Tanzania. TropMedIntHealth 12: 547–553.
  7. 7. Bousema JT, Schneider P, Gouagna LC, Drakeley CJ, Tostmann A, et al. (2006) Moderate Effect of Artemisinin-Based Combination Therapy on Transmission of Plasmodium falciparum. JInfectDis 193: 1151–1159.
  8. 8. Bonnet S, Gouagna LC, Paul RE, Safeukui I, Meunier JY, et al. (2003) Estimation of malaria transmission from humans to mosquitoes in two neighbouring villages in south Cameroon: evaluation and comparison of several indices. TransRSocTropMedHyg 97: 53–59.
  9. 9. Gamage-Mendis AC, Rajakaruna J, Carter R, Mendis KN (1991) Infectious reservoir of Plasmodium vivax and Plasmodium falciparum malaria in an endemic region of Sri Lanka. American Journal of Tropical Medicine and Hygiene 45: 479–487.
  10. 10. Boudin C, Olivier M, Molez JF, Chiron JP, Ambroise-Thomas P (1993) High human malarial infectivity to laboratory-bred Anopheles gambiae in a village in Burkina Faso. AmJTropMedHyg 48: 700–706.
  11. 11. Young MD, Hardman NF, Burgess RW, Frohne WC, Sabrosky CW (1948) The infectivity of native malarias in South Carolina to Anopheles quadrimaculatus. Am J Trop Med Hyg 28: 303–311.
  12. 12. Pethleart A, Prajakwong S, Suwonkerd W, Corthong B, Webber R, et al. (2004) Infectious reservoir of Plasmodium infection in Mae Hong Son Province, north-west Thailand. Malar J 3: 34.
  13. 13. Jeffery GM, Eyles DE (1955) Infectivity to mosquitoes of Plasmodium falciparum as related to gametocyte density and duration of infection. AmJTropMedHyg 4: 781–789.
  14. 14. Bonnet S, Gouagna C, Safeukui I, Meunier JY, Boudin C (2000) Comparison of artificial membrane feeding with direct skin feeding to estimate infectiousness of Plasmodium falciparum gametocyte carriers to mosquitoes. TransRSocTropMedHyg 94: 103–106.
  15. 15. Graves PM (1980) Studies on the use of a membrane feeding technique for infecting Anopheles gambiae with Plasmodium falciparum. TransRSocTropMedHyg 74: 738–742.
  16. 16. Coleman RE, Kumpitak C, Ponlawat A, Maneechai N, Phunkitchar V, et al. (2004) Infectivity of asymptomatic Plasmodium-infected human populations to Anopheles dirus mosquitoes in western Thailand. JMedEntomol 41: 201–208.
  17. 17. Githeko AK, Brandling-Bennett AD, Beier M, Atieli F, Owaga M, et al. (1992) The reservoir of Plasmodium falciparum malaria in a holoendemic area of western Kenya. TransRSocTropMedHyg 86: 355–358.
  18. 18. Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, et al. (2007) Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. AmJTropMedHyg 76: 470–474.
  19. 19. Muirhead-Thomson RC (1954) Factors determining the true reservoir of infection of Plasmodium falciparum and Wuchereria bacnrofti in a West African village. Transactions of the Royal Society of Tropical Medicine and Hygiene 48: 208–225.
  20. 20. Okell LC, Drakeley CJ, Ghani AC, Bousema T, Sutherland CJ (2008) Reduction of transmission from malaria patients by artemisinin combination therapies: a pooled analysis of six randomized trials. MalarJ 7: 125.
  21. 21. Cuzin-Ouattara N, Van den Broek AH, Habluetzel A, Diabate A, Sanogo-Ilboudo E, et al. (1999) Wide-scale installation of insecticide-treated curtains confers high levels of protection against malaria transmission in a hyperendemic area of Burkina Faso. Trans R Soc Trop Med Hyg 93: 473–479.
  22. 22. Ouedraogo AL, De Vlas SJ, Nebie I, Ilboudo-Sanogo E, Bousema JT, et al. (2008) Seasonal patterns of Plasmodium falciparum gametocyte prevalence and density in a rural population of Burkina Faso. Acta Trop 105: 28–34.
  23. 23. Ouedraogo AL, Schneider P, de Kruijf M, Nebie I, Verhave JP, et al. (2007) Age-dependent distribution of Plasmodium falciparum gametocytes quantified by Pfs25 real-time QT-NASBA in a cross-sectional study in Burkina Faso. AmJTropMedHyg 76: 626–630.
  24. 24. Schneider P, Bousema T, Omar S, Gouagna L, Sawa P, et al. (2006) (Sub)microscopic Plasmodium falciparum gametocytaemia in Kenyan children after treatment with sulphadoxine-pyrimethamine monotherapy or in combination with artesunate. IntJParasitol 36: 403–408.
  25. 25. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, et al. (1990) Rapid and simple method for purification of nucleic acids. JClinMicrobiol 28: 495–503.
  26. 26. Ponnudurai T, Lensen AH, Meis JF, Meuwissen JH (1986) Synchronization of Plasmodium falciparum gametocytes using an automated suspension culture system. Parasitology 93 (Pt 2): 263–274.
  27. 27. Bousema JT, Gouagna LC, Drakeley CJ, Meutstege AM, Okech BA, et al. (2004) Plasmodium falciparum gametocyte carriage in asymptomatic children in western Kenya. Malaria Journal 3: 18.
  28. 28. Pichon G, Awono-Ambene HP, Robert V (2000) High heterogeneity in the number of Plasmodium falciparum gametocytes in the bloodmeal of mosquitoes fed on the same host. Parasitology 121 (Pt 2): 115–120.
  29. 29. Bousema JT, Drakeley CJ, Sauerwein RW (2006) Sexual-Stage Antibody Responses to P. falciparum in Endemic Populations. CurrMolMed 6: 223–229.