Open Access

Research Article

Introduction Top

Theoretical models of the mammalian jaw apparatus predict that bite force is intimately linked to jaw gape angle and bite position [1]–[4]. Shallower gapes and more proximal tooth positions should lead to greater overall force production. While some validation has been forwarded with respect to humans,[5] few studies have examined relationships between these factors in other vertebrate species and none have investigated their influence on cranial stress distributions. Many studies have focused only on maximum bite force [6]–[8]. Tests on how force varies along the jaw line, and between gape angles, have been few and far between [9]–[12] and none have concentrated on carnivoran mammals. It has been argued that carnivoran muscle insertion geometry, and mandibular articulation angles might facilitate the generation of greater bite forces at wider angles than in more generalized mammals [12]–[13]. Thus, it is possible that carnivoran skull mechanics don't follow patterns deduced for other taxa.

Finite Element (FE) analysis has become increasingly popular as a means of examining biomechanical questions [13]–[18]. The non-destructive, malleable nature of FE models and their capacity to reveal detailed information has allowed researchers to test biomechanical scenarios that would be extremely time-consuming, dangerous, or ethically challenging using available in vivo technologies 19–21.

Our aim in the present study has been to apply a Finite Element approach to examine the affect of both gape angle and bite position on bite force and to map the influence of variation in these factors on cranial stress in a relatively generalized carnivoran mammal. The subject used was an Australian dingo (Canis lupus dingo).

Materials and Methods Top

We used an FE model of a dingo skull (AM 38587) previously assembled by Wroe et al [22] from computerized serial tomography data. This model comprised eight material properties incorporated on the basis of density data [22]. Seven iterations of this model were generated using Strand7 (Vers. 2.3) in which gape angles differed by 10 degrees, ranging from 65° (maximal gape) to 5°.

Restraint and rigid link assignment were as in Wroe et al [22] To prevent free body rotation, and more broadly distribute force, a framework of rigid links was placed at the occipital condyle, and on tooth bite points. A linear static test was performed on each model. Two bite transmitted load cases were used. They consisted of bites that were driven solely by the skull musculature, with maximal bite force being assumed for each instance. The two intrinsic load cases used were a bilateral bite at the canines, and a bilateral bite at the carnassials. Muscle forces and architecture were approximated through the addition of pretensioned trusses. These are beam elements, that carry axial loads only. Truss element numbers and diameter were as in Wroe et al [22].

Mean brick element stress was obtained from six regions of the skull. These regions were: the entire skull, the cranium (skull sans the mandible), the rostrum (the region from the antorbital fenestra, to the anterior-most tip of the cranium), the anterior orbit (anterior margin of orbit, to anterior fenestra), the zygomatic arch and the mandible. These regions were chosen for the symmetrical stress distributions that they produced under bilateral load cases.

Mechanical behaviour was determined by the visual output of the post-processing software and mean brick stress values. All data was calculated in terms of Von Mises (VM) stress. Von Mises stress is a function of the principal stresses (σ₁, σ₂, & σ₃) that measures how stress distorts material. Failure of ductile material, such as bone, is estimated when VM stress equals the yield strength of the material in uniaxial tension [14]. Since Von Mises stress is proportional to strain energy, [12] it can be used to determine the amount of strain placed on bone under various load cases.

Statistical analysis was performed using a customized program written in RGUI by Karen Moreno.

Results Top

Bite Force

A negative correlation was found between bite force and gape angle, with shallower angles producing higher bite forces for both canines and carnassials (table 1). Bite force between carnassials and canines varied substantially. On average, carnassial bites produced forces 2.4 times greater than those of canines under similar conditions (fig 1).

Figure 1. Comparison of canine derived bites, vs carnassial bites.

doi:10.1371/journal.pone.0002200.g001

Table 1. Comparison of gape angle and bite point on overall bite force in C. l. dingo.

doi:10.1371/journal.pone.0002200.t001

Stress

Mean brick stress was superficially similar under both load cases. Stress tended to increase as jaw angle decreased (fig 2). The mandible showed the greatest increase in stress out of all the skull regions, with shallower gape angles delivering the largest VM values. VM stress was consistently high along the edges of the coronoid process, and near the base of the mandibular condyle. These stress values were directly muscle related, and could be viewed as an artifact of the muscle modeling [19]. That said, there was a noted increase in stress along these regions of the mandible. This suggested a greater pull being generated by the temporalis and masseter during these shallow bite tests. The zygomatic arch showed the highest VM stress for the cranium (table 2); with stress levels remaining mostly stable throughout both load cases, and all jaw positions. VM stress was always higher in canine bites than respective carnassial bites (fig 3).

Figure 2. Comparison of stress distribution along the skull during each freedom case.

doi:10.1371/journal.pone.0002200.g002

Figure 3. Comparison of stress development in C.l.dingo during bites directed at the canines (left), or carnassials (right) at select angles to show trend.

Gape angles are (a) 65°, (b) 35° and (c) 05°.

doi:10.1371/journal.pone.0002200.g003

Table 2. Mean brick stress (VM) of select skull regions during static bite tests at various angles and jaw regions for solved FE models for C. l. dingo.

doi:10.1371/journal.pone.0002200.t002

Differences between the bite points were also observed in the cranium. For the canine load case, VM stress was seen to increase linearly in the cranium as gape shallowed. However once the jaw reached a 25° angle to the rest of the skull, cranial stress plateaued, with VM stress increasing by no more than 1% between 25° and 5°. The carnassial load case showed a different pattern in the cranium. With the carnassials, there was a notable “dip” in stress starting at 55° and continuing down to 45° where VM values were at their lowest (fig 4).

Figure 4. Focus on cranium stress distribution during each freedom case.

doi:10.1371/journal.pone.0002200.g004

Discussion Top

The data from this study lends support to work using more traditional methods that showed bite force to correlate negatively with jaw gape [4]–[5], [19]. That moving closer to the pivot point of the jaw would increase bite strength is an expected result of the jaw's lever mechanics [11], [23]. Although the largest bite force was generated at the shallowest jaw gape, the greatest increase in bite force did not occur at this angle. Rather it occurred at 25° for the canines and 35° for the carnassials.

It is notable that these angles of high bite force increase also appeared to correlate with regions of the cranium that showed either resistance to the increased force (canine bites), or lowered VM stress (carnassial bites). This suggests that the dingo's skull and musculature may be optimized to deliver maximal bites at these angles, with interesting functional implications. Among subspecies of grey wolf and social canids in general, bite force in the dingo is relatively weak and bite force adjusted for body mass allometry has been shown to correlate with prey size [24]–[25]. We suggest that optimal gape angle may also be a useful indicator of feeding ecology among carnivorous mammals. Analyses incorporating both large prey specialists (e.g., Canis lupus lupus, Lycaon pictus) and small prey specialists (e.g., Vulpes vulpes) are needed to examine this proposal. Alternatively it could also be that optimality in this range is necessary for disabling bites, such as the severing of major tendons.

As the mandible is the primary object being powered by the jaw muscles, finding higher VM stress in this skull region is not entirely surprising. It is doubtful, however, that stress increase was solely due to the higher bite force attributed to the more acute angles. If bite force itself was the main driver of mandibular stress, then VM stress in the mandible would be greatest in the much stronger carnassial bites. However, a carnassial bite at maximum gape, producing a force 2.7 times higher than the respective canine bite, resulted in mandibular VM stress that was 30% lower than in a canine bite at the same angle. This suggested that dingoes, and perhaps by extension, carnivorans in general, have evolved skulls that are better adapted to tolerate stresses at wider jaw angles than other mammals.

Overall, the results of this study offer support for previous models of mammalian jaw mechanics [1]–[4]. Bite force is markedly affected by both jaw gape and point of contact along the jaw line. Proximal bites and acute jaw angles result in greater overall force. Even for carnivorans, which have undergone evolutionary adaptations to allow for greater bite forces at wider gape angles, the rules of the models remains true.

In conclusion, while bite force in C. l. dingo appears to still be limited by overall jaw mechanics, the fact that stress data shows greater tolerance of wide jaw angles, indicates the direction taken in carnivoran evolution. Consideration of stress and bite force data, suggests that there is an optimal bite angle of between 25° and 35° in C. l. dingo. Further analyses will be needed to determine whether optimal gape angle might be a useful predictor of feeding ecology.

References Top

1. Pruim GJ, DeJongh HJ, Ten Bosch JJ (1980) Forces Acting on the Mandible During Bilateral Static Biting at Different Bite Force Levels. J Biomech 13: 755–763. Find this article online
2. Herring SW, Herring SE (1974) The Superficial Masseter and gape in Mammals. Amer Nat 108: 561–576. Find this article online
3. Lindauer SJ, Gay T, Rendell J (1993) Effect of Jaw Opening on Masticatory Muscle EMG-Force Characteristics. J Dental Res 72: 51–55. Find this article online
4. Greaves WS (1982) A Mechanical Limitation on the Position of the Jaw Muscles in Mammals: The One Third-Rule. J Mammalogy 63 (2): 261–266. Find this article online
5. Fields HW, Proffitt WR, Case JC, Vig KWL (1986) Variables Affecting Measurements of Vertical Occlusal Force. J Dental Res 62: 135–138. Find this article online
6. Dechow PC, Carlson DS (1983) A Method of Bite Force Measurement in Primates. J Biomech 16: 797–802. Find this article online
7. Aguirre LF, Herrel A, Van Damme R, Matthysen E (2002) Ecomorphological Analysis of Trophic Niche Partitioning in a Tropical Savannah Bat Community. Proc R Soc Lond B 269: 1271–1278. Find this article online
8. Binder WJ, Van Valkenburgh B (2000) Development of Bite Strength and Feeding Behaviour in Juvenile Spotted Hyenas (Crocuta crocuta). J Zool 252: 273–283. Find this article online
9. Hylander WL (1977) In Vivo Bone Strain in the Mandible of Galago crassicaudatus. Am J Phys Anthropol 46: 309–326. Find this article online
10. Hylander WL (1979) Mandibular Function in Galago crassicaudatus and Macaca fasicularis. An In Vivo Approach to Stress Analysis of the Mandible. J Morphol 159: 253–296. Find this article online
11. Thompson EN, Biknevicius AR, German RZ (2003) Ontogeny of Feeding Function in the Gray Short-Tailed Opossum Monodelphis domestica: Empirical Support for the Constrained Model of Jaw Biomechanics. J Exp Biol 206: 923–932. doi: 10.1242/jeb.00181. Find this article online
12. Dumont ER, Herrel A (2003) The Effects of Gape Angle and Bite Point on Bite Force in Bats. J Exp Biol 206: 2117–2123. doi: 10.1242/jeb.00375. Find this article online
13. Emerson SB, Radinsky L (1980) Functional Analysis of Sabertooth Cranial Morphology. Paleobiology 6(3): 295–312. Find this article online
14. Rayfield EJ (2007) Finite Element Analysis and Understanding the Biomechanics and Evolution of Living and Fossil Organisms. Annu rev Earth Planet Sci 35: 541–576. Find this article online
15. Strait DS, Wang Q, Dechow PC, Ross CF, Richmond BG, et al. (2005) Modeling Elastic Properties in Finite Element Analysis: How Much Precision is needed to Produce an Accurate Model? Anat Rec 283A: 275–287. Find this article online
16. Ross CF, Patel BA, Slice DE, Strait DS, Dechow PC, et al. (2005) Modeling Masticatory Muscle Force in Finite Element Analysis: Sensitivity Analysis Using Principal Coordinates Analysis. Anat Rec 283A: 288–299. Find this article online
17. Thomason JJ, McClinchey HL, Faramarzi B, Jofriet JC (2005) Mechanical Behavior and Quantitative Morphology of the Equine Laminar Junction. Anat Rec 283A: 366–379. Find this article online
18. Wroe S. Cranial mechanics compared in extinct marsupial and extant African lions using a finite-element approach. Journal of Zoology (London). In Press. Find this article online
19. Dumont ER, Piccirillo J, Grosse IR (2005) Finite-Element Analysis of Biting Behavior and Bone Stress in the Facial Skeletons of Bats. Anat Rec 283A: 319–330. Find this article online
20. McHenry CR, Wroe S, Clausen PD, Moreno K, Cunningham E (2007) Supermodeled Sabercat, Predatory Behavior in Smilodon fatalis Revealed by High-Resolution 3D Computer Simulation. PNAS 104. 41: 16010–16015. doi: 10.1073/pnas.0706086104. Find this article online
21. Wroe S, Moreno K, Clausen P, McHenry C, Curnoe D (2007) High-Resolution Three-Dimensional Computer Simulation of Hominid Cranial Mechanics. Anat Rec 290: 1248–1255. Find this article online
22. Wroe S, Clausen P, McHenry C, Moreno K, Cunningham E (2007) Computer simulation of feeding behaviour in the thylacine and dingo as a novel test for convergence and niche overlap. Proc R Soc B Vol. 274. 1627: 2819–2828. doi:10.1098/rspb.2007.0906. Find this article online
23. Husak JF, Lappin AK, Fox SF, Lemos-Espinal JA (2006) Bite-Force Performance Predicts Dominance in Male Venerable collared Lizards (Crotaphytus antiquus). Copeia 2: 301–306. Find this article online
24. Wroe S, McHenry C, Thomason J (2005) Bite club: Comparative bite force in big biting mammals and the prediction of predatory behaviour in fossil taxa. Proceedings of the Royal Society of London, Series B 272: 619–625. Find this article online
25. Christiansen P, Wroe S (2007) Bite force predicts feeding ecology in carnivorans. Ecology 88: 347–358. Find this article online