Figures
Abstract
Mast cells (MCs) are heterogeneous cells whose phenotype is modulated by signals received from the local microenvironment. Recent studies have identified the mesenchymal-derived cytokine IL-33 as a potent direct activator of MCs, as well as regulator of their effector phenotype, and have implicated this activity in the ability of mast cells to contribute to murine experimental arthritis. We explored the hypothesis that IL-33 enables participation of synovial MCs in murine K/BxN arthritis by promoting their activation by IgG immune complexes. Compared to wild-type (WT) control mice, transgenic animals lacking the IL-33 receptor ST2 exhibited impaired MC-dependent immune complex-induced vascular permeability (flare) and attenuated K/BxN arthritis. Whereas participation of MCs in this model is mediated by the activating IgG receptor FcγRIII, we pre-incubated bone marrow-derived MCs with IL-33 and found not only direct induction of cytokine release but also a marked increase in FcγRIII-driven production of critical arthritogenic mediators including IL-1β and CXCL2. This “priming” effect was associated with mRNA accumulation rather than altered expression of Fcγ receptors, could be mimicked by co-culture of WT but not ST2−/− MCs with synovial fibroblasts, and was blocked by antibodies against IL-33. In turn, WT but not ST2−/− MCs augmented fibroblast expression of IL-33, forming a positive feedback circuit. Together, these findings confirm a novel role for IL-33 as an amplifier of IgG immune complex-mediated inflammation and identify a potential MC-fibroblast amplification loop dependent on IL-33 and ST2.
Citation: Kaieda S, Wang J-X, Shnayder R, Fishgal N, Hei H, Lee RT, et al. (2012) Interleukin-33 Primes Mast Cells for Activation by IgG Immune Complexes. PLoS ONE 7(10): e47252. https://doi.org/10.1371/journal.pone.0047252
Editor: Jose Carlos Alves-Filho, University of São Paulo, Brazil
Received: May 11, 2012; Accepted: September 12, 2012; Published: October 11, 2012
Copyright: © Kaieda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by NIH grants AR051321 (to P.A.N.), AI059746 (to P.A.N. and R.L.S.), HL092930 (to R.T.L.), and AI065858 (to R.L.S.), and by the Cogan Family Foundation (to P.A.N.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Mast cells (MCs) are hematopoietic cells that develop from circulating progenitors and differentiate into fully granulated effector cells within the local tissue milieu. The survival, development, phenotype, and function of these immune cells are modulated by contact-dependent and -independent signals from the microenvironment [1], [2], [3], [4]. In many locations, MCs reside in close proximity to fibroblasts, and prior work has demonstrated the existence of intricate physical contacts between the two cell types [5], [6], [7]. This interaction is of critical importance to MCs, since expression of membrane-bound Kit ligand (KitL) by fibroblasts enables the survival of MCs in tissues [8], [9]. Fibroblasts also modulate the effector phenotype of MCs, including their expression of eicosanoids and granule proteases [5], [6]. In turn, MCs influence the growth and activation of fibroblasts [10], [11].
Among the multiple factors known to influence MC phenotype and behavior, recent interest has focused on IL-33, a pro-inflammatory member of the IL-1 cytokine family [12], [13]. IL-33 is produced primarily by fibroblasts, smooth muscle cells, keratinocytes, and endothelial cells; MCs themselves have also been identified as a potential source [14], [15]. Acting via its receptor ST2, IL-33 triggers MCs to release numerous cytokines and chemokines [16], [17], [18], [19], [20], [21], [22], an activity implicated in the pathogenesis of anaphylaxis and in the role of mast cells as sensors of tissue injury [22], [23]. Moreover, exposure of MCs to IL-33 augments expression of cytokines in MCs activated concomitantly via the high-affinity IgE receptor FcεRI [24], [25]. IL-33 is also the first factor shown to promote the accumulation in granules of mouse MC protease 6 [26], an ortholog of human tryptase β that plays a role in innate immunity and inflammatory arthritis [27], [28], [29]. Thus, IL-33 also is a granule maturation factor for MCs.
Recent studies have implicated IL-33 in the activation of synovial MCs in murine arthritis [21], [30], [31]. Transgenic mice lacking ST2 exhibit impaired degranulation of synovial MCs, while MCs cultured overnight in the presence of arthritogenic K/BxN mouse serum have been reported to become susceptible to IL-33-induced degranulation [31]. However, MC-dependent vasogenic edema begins within minutes of the administration of K/BxN mouse serum, a time course that may be too rapid for de novo release of IL-33 [14], [32], [33], [34]. Further, genetic studies have demonstrated that FcγRIII is an obligate pathway for the activation of synovial MCs in K/BxN arthritis [33], [35]. It therefore remains unclear, in the context of arthritis, whether the major role of IL-33 is to activate MCs directly or rather to potentiate their activation via other pathways, such as Fc receptors. To address this question, we tested the role of IL-33 in the response of MCs to FcγRIII ligation, and explored the role of this cytokine in the interactions between MCs and synovial fibroblasts, a potential source of IL-33 within the joint.
Materials and Methods
Reagents
The Mouse Cytokine Array Panel A, recombinant mouse IL-33, and specific ELISAs for CXCL2, tumor necrosis factor-α (TNF-α), IL-1β, IL-6, IL-33 and anti-IL-33 Ab (MAB3626) were obtained from R&D Systems (Minneapolis, MN). Mouse IgG1 isotype antibody was from Biolegend (San Diego, CA). Anti-IL-33 (Nessy-1) was from Enzo Life Sciences (Farmingdale, NY). Recombinant mouse IL-3 and KitL were obtained from Peprotech (Rocky Hill, NJ), and endotoxin-free rat anti-mouse FcγRII/III antibody (Ab) (clone 2.4G2) was obtained from BioXCell (West Lebanon, NH). Additional reagents included 2.4G2-PE Ab (BD Biosciences, San Diego, CA) and rat anti-FcγRIII Abs (clone 275003, unconjugated and carboxyfluorescein conjugated, R&D Systems).
Mice
Wild-type (WT) C57BL/6J (B6) and B6;129S4-Fcγr2btm1Ttk/J (FcγRII−/−) mice were from the Jackson Labs (Bar Harbor, ME). ST2−/− B6 mice were obtained from Dr. Andrew McKenzie (MRC Laboratory of Molecular Biology) [36]. Animal experiments were approved by the Institutional Animal Care and Use Committee of the Dana Farber Cancer Institute (Animal Welfare Assurance Number: A3023-01). All efforts were made to minimize the suffering of animals used in this research.
Experimental Arthritis
Pro-arthritic serum was isolated from K/BxN mice as previously described [37]. K/BxN arthritis was induced by the intraperitoneal injection of diluted K/BxN serum (75 µl serum with 75 µl endotoxin-free PBS) on days 0 and 2 of each experiment. Arthritis was graded using a 0–12 clinical scale (0–3 per paw) as well as by caliper measurement of ankle thickness, as described [37]. “Flare” (acute paw swelling) was measured by caliper in all 4 paws 30 minutes after the first serum administration [33]. Histological assessment was performed on paraffin-embedded 4-µm sections stained with hematoxylin and eosin, and synovial inflammation, cartilage injury, and bone erosion were graded in blinded fashion on a 0–5 scale using an established system [35].
Cell Culture and Mast Cell Activation
Mouse bone marrow derived MCs (mBMMCs) were developed by culturing bone marrow cells for at least 4 weeks in 10% FBS DMEM media supplemented with IL-3 (10 ng/ml) and KitL (25 ng/ml), as previously described [37]. Fibroblast-like synoviocytes (FLS) were cultured from collagenase-digested mouse ankles in 10% FBS DMEM media [38]. For co-culture experiments where FLS are a potential source of KitL, mBMMCs were derived as above but using only IL-3 (10 ng/ml) [39]. Co-culture of IL-3-developed mBMMCs and FLS was performed in the presence of 10 ng/ml recombinant IL-3, with the cell types separated by a membrane containing 0.2 µm pores [26]. Activation of IL-3/KitL-developed mBMMCs via FcγRIII was achieved by low-speed centrifugation against plate-bound 2.4G2 Ab as described [35]. For these experiments, cells were maintained in their pre-incubation medium (i.e., containing IL-33 if added, as well as any mediators released through the action of IL-33 prior to crosslinking of FcγRIII by plate-bound 2.4G2). Quantification of cytokine array spot density for the Mouse Cytokine Array Panel A was performed using NIH ImageJ and normalized to positive controls in each membrane as per manufacturer instructions.
Real-time Quantitative PCR (qPCR)
Total RNA was isolated from mBMMCs, FLS, and ankle joints using the RNeasy minikit (Qiagen, Valencia, CA), followed by qPCR employing primers specific for IL-6, IL-1β, TNF-α, IL-33, CXCL2, β-Actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [26], [33], [40].
Statistical Analysis
P values were calculated by Student’s t-test, where normality was confirmed using the Kolmogorov-Smirnov test, by Mann-Whitney for non-parametric data, and by ANOVA for curves (GraphPad Prism, version 4.0). Synergistic interaction between stimuli was evaluated using linear regression (SAS, version 9.2). P values smaller than 0.05 were considered significant.
Results
ST2 Promotes Disease Severity and Mast Cell Activation in K/BxN Arthritis
To explore the role of IL-33 in K/BxN arthritis and to evaluate early immune complex-mediated activation of MCs, we induced arthritis in mice lacking ST2 as well as in WT control mice. In agreement with published data [31], we found that ST2−/− animals exhibited less severe arthritis (Figures 1A and 1B). Correspondingly, histological measures of arthritis were reduced, as was tissue expression of IL-6, IL-1β and TNF-α (Figures 1C and 1D). Congruent with the prior demonstration in arthritic day 4 tissues that MC degranulation is impaired by ST2 deficiency [31], we found that acute MC-dependent vascular edema (“flare”) [33] was reduced in the transgenic mice 30 minutes after serum administration (Figure 1E). These results confirm the importance of ST2 in this model, including in initial MC activation.
Arthritis was initiated in ST2−/− mice and their WT littermates via intraperitoneal administration of K/BxN mouse serum on days 0 and 2 (n = 5/group). (A) Clinical score on a 0–12 scale, P<0.0001, WT versus ST2−/−. (B) Change in ankle thickness, P<0.0001, WT versus ST2−/−. (C) Histomorphometric quantification of arthritic tissue (5 ankles/group). (D) Cytokine mRNA in ankle lysates (10 ankles/group from two separate experiments) at day 8 or 10 arthritis. (E) Acute change in wrist and ankle thickness (“flare”) measured 30 minutes after initial serum administration (n = 5/group). Results shown are the mean ± SEM. Panels A–C&E reflect 1 of 2 experiments with similar results. *P<0.05, **P<0.01, WT versus ST2−/−.
IL-33 Amplifies FcγRIII-mediated Mast Cell Mediator Production
FcγRIII and FcγRII are stimulatory and inhibitory IgG receptors, respectively, that mediate opposing effects on immune complex-induced MC activation. In K/BxN arthritis, engraftment experiments have shown that effective engagement of synovial MCs requires the activating IgG receptor FcγRIII [35]. Further, synovial MCs lacking FcγRIII fail to degranulate upon serum administration [33]. Expression of the C5a complement receptor CD88 by MCs is also required, though at a step downstream of degranulation [33]. To assess whether IL-33 enhances MC activation via FcγRIII, we employed an established in vitro system [35]. WT mBMMCs and transgenic mBMMCs lacking FcγRII were cultured for 4 hours in the presence or absence of IL-33. The resulting cells were then activated via plate-bound anti-FcγRII/III Ab (clone 2.4G2). IL-33 markedly amplified IL-6 production by FcγRII−/− cells (Figure 2A). Interestingly, whereas activation of WT mBMMCs by 2.4G2 is typically blocked due to engagement of the inhibitory receptor FcγRII, IL-33 enabled MC to partially bypass this blockade and respond productively to this immune complex mimic (Figure 2B). Of note, we failed to observe any effect of IL-33 on exocytosis of the granule constituent β-hexosaminidase (Figure 2C), a defect that could not be reversed using published methods [23], [31], including overnight pre-incubation with IgE (anti-trinitrophenyl, 0.3 µg/ml) or K/BxN mouse serum (1∶100-1∶1) (data not shown). We also could not identify a synergistic effect of exogenous IL-33 and C5a in our mBMMC, with or without concomitant FcγRII/III ligation (data not shown).
(A–C) FcγRII−/− or WT B6 mBMMCs were pre-incubated with or without IL-33 (10 ng/ml) for 4 hours, and then cells in pre-incubation media were spun onto plates pre-coated with anti-FcγRII/III Ab (2.4G2). Supernatants were harvested at 16 hours and assayed for IL-6 (A&B) and the granule mediator β-hexosaminidase (C). Differences in baseline IL-6 production were reproducibly observed between FcγRII−/− and B6 mBMMCs, and may reflect divergent genetic backgrounds or other factors. (D) FcγRII−/− mBMMCs incubated with or without IL-33 for 4 hours were activated by plate-bound 2.4G2 Ab (10 µg/ml) for 16 hours and assayed by multiplex cytokine array. (E) Quantitation of optical density of selected mediators from D (mean of 2 dots). (F) Assay of mediators identified in D-E via specific ELISA in separate experiments employing an identical experimental design (IL-1β performed on lysates). (G) To determine whether IL-33 induced intracellular accumulation of cytokine, B6 mBMMCs were stimulated with IL-33 (10 ng/ml) for the intervals indicated, washed×2 in ice-cold PBS, and lysed in the presence of protease inhibitors. All results representative of at least 2 independent experiments. *P<0.05, **P<0.01, ***P<0.001.
To understand more fully the effect of cooperative IL-33- and FcγRIII-mediated activation of MCs, we employed a multiplex array to evaluate cytokines and chemokines elaborated by 2.4G2-stimulated FcγRII−/− mBMMCs, pre-incubated with IL-33 or vehicle for 4 hours. The results confirmed our IL-6 data, and showed that amplification extended to other mediators as well, most prominently TNF-α and CXCL2 (Figure 2D, quantitated in Figure 2E). The latter finding is particularly interesting, since arthritis is neutrophil dependent and the receptor CXCR2 has been implicated in IL-33-mediated neutrophil recruitment [41], [42], [43]. Selected results were further confirmed by specific ELISAs (Figure 2F). Whereas IL-1β has been shown to be a key MC-derived arthritogenic cytokine [35], we assayed for IL-1β in lysates of FcγRIII-stimulated FcγRII−/− mBMMCs and found a marked increase after IL-33 pre-incubation (Figure 2F). Linear regression demonstrated that the effect of sequential stimulation by IL-33 followed by FcγRIII ligation far exceeded an additive effect, confirming synergy (TNF-α P = 0.0005, IL-1β and CXCL2 P<0.0001). Interestingly, in mBMMCs stimulated with IL-33 and subsequently washed to remove released cytokine, accumulation of intracellular cytokine was observed for IL-6 and IL-1β, but not TNF-α (Figure 2G).
Mast Cell Priming by IL-33 Reflects mRNA Accumulation Rather than FcγR Expression
Exploring the mechanism of the observed synergy, we examined whether IL-33 pre-incubation altered FcγR expression and/or downstream processes involved in the expression, biosynthesis, and release of mediators. Reciprocal modulation of FcγRII and FcγRIII expression is a well-recognized pathway for enhancing the responsiveness of cells to immune complexes [44], although we have been unable to confirm that this mechanism is active in either mouse or human MCs [33]. Exposure of mBMMCs to IL-33 failed to alter surface expression of FcγRII or FcγRIII (Figure 3A), consistent with the lack of an effect of IL-33 on FcR-mediated MC activation threshold (Figure 2C). Rather, pre-incubation of mBMMCs with IL-33 induced a marked accumulation of transcripts that encode numerous pro-inflammatory factors. Most remarkably, the level of IL-1β transcript increased several hundred-fold, an effect that could be observed as low as 3–10 pg/ml of IL-33 (Figure 3B and data not shown). We interpret these results to indicate “priming” of MCs by IL-33, whereby exposure of MC to IL-33 alters the state of the cells to enable markedly enhanced production of pro-inflammatory mediators upon subsequent stimulation via FcγRIII.
(A) B6 mBMMCs were incubated with IL-33 (10 ng/ml) and analyzed for surface expression of inhibitory (FcγRII) and stimulatory (FcγRIII) IgG receptors by flow cytometry at the indicated time points. (B) Cytokine mRNA expression in B6 mBMMCs exposed to IL-33 (10 ng/ml) for 4 hours. Results reflect at least 2 independent experiments.
IL-33 and ST2 Mediate Mast Cell Priming by Fibroblasts
Whereas IL-33 may be elaborated by synovial fibroblasts [21], [30], we explored the possibility that this cytokine could be pivotal for MC-fibroblast interactions. For these experiments, we co-cultured mBMMCs and FLS in an established transwell system that prohibited direct cell-cell contact and permitted separate analysis of each cell type [26]. We found that FLS induced mBMMCs to increase their levels of transcripts for IL-6 and IL-1β. This effect was completely abrogated in ST2-deficient MCs (Figure 4A). Whereas IL-33 is potent at low concentrations and unstable to manipulation, we were unable to measure this cytokine consistently in our extended co-culture supernatants (detection limit 10–15 pg/ml). Therefore, we repeated our studies in the presence of blocking antibodies against IL-33 [45]. Consistent with our ST2−/− findings, specific IL-33 antagonism abrogated mRNA accumulation in WT MCs (Figure 4B). These results implicate IL-33, acting via ST2, as the key soluble mediator driving accumulation of pro-inflammatory cytokine mRNA in MC in our co-culture system.
MCs were cultured with or without FLS for 1–2 weeks in the upper and lower chambers, respectively, of a transwell apparatus. (A) Cytokine mRNA expression in WT and ST2−/− MCs after co-culture with FLS. (B) Cytokine mRNA levels in MCs with or without anti-IL-33 Ab treatment (10 µg/ml q4d). Data represent 2 independent experiments with similar results. (C–D) Cytokine mRNA levels in FLS. n = 2 wells per condition, reflective of 2–5 pooled experiments. *P<0.05, **P<0.01, N.S., not significant.
Interestingly, mBMMCs induced co-cultured FLS to increase their expression of IL-33 and IL-6 (Figure 4C). This reciprocal effect on FLS also required MCs to express ST2 (Figure 4D), indicating an ST2-dependent MC-FLS pro-inflammatory loop. Whereas MCs have recently been identified as a potential source of IL-33 [15], we assessed IL-33 mRNA from co-cultured mBMMCs in two experiments and found it to be either low (<0.03 vs. GAPDH) or absent (<0.0002 vs. GAPDH), indicating that FLS are the most likely source of IL-33 in our system. Of note, neutralizing antibodies against IL-6 and IL-1β failed to abrogate the loop (data not shown). Therefore, the identity of the MC-derived soluble factor(s) mediating IL-33 mRNA up-regulation in FLS remains to be determined.
Discussion
Among their many functions, MCs are immune sentinels, residing near epithelial surfaces, blood vessels, and near vulnerable body cavities where they serve to provide surveillance against pathogen invasion, tissue injury, and other insults [13], [46]. Upon activation, MCs can elaborate a range of responses depending not only upon the stimulus but also upon their particular phenotype [4]. MCs from different tissue sites express distinct surface receptors, intracellular proteases, and other effector molecules. These phenotypic changes are mediated by the local environment, though the detailed pathways involved are incompletely defined.
Here, we identify a new role for IL-33 and its receptor ST2 in IgG-mediated MC activation. We previously showed that MCs activated via FcγRIII elaborate IL-1β, and that this pathway is required for MCs to “jump start” IgG-mediated K/BxN murine arthritis [35]. However, the quantity of IL-1β found to be elaborated by cultured MCs stimulated in vitro via FcγRIII was smaller than might have been expected given the prominent in vivo role of this cytokine. The present work helps to bridge this gap. We now show that exposure of MCs to IL-33 dramatically increased their production of IL-1β upon FcγRIII ligation, and that this effect could be mimicked by co-culture with primary fibroblasts derived from mouse synovium. Further, we found that this relationship was reciprocal, in that MCs activated via ST2 augmented IL-33 expression in co-cultured FLS, representing a potential amplification loop (Figure 5). The in vivo importance of these in vitro observations was suggested by the reduced intensity of K/BxN arthritis in ST2−/− mice, a phenotype associated by others with altered MC activation [31].
In the model proposed, synovial fibroblasts release IL-33 in a constitutive or induced manner. IL-33 causes phenotypic changes in neighboring MCs, including accumulation of cytokine mRNA and alteration in granule content, depicted as color change in “primed” MC. Upon exposure to immune complexes, primed MCs exhibit release pro-inflammatory mediators that further activate fibroblasts, promote neutrophil recruitment, and contribute to arthritis severity. Reciprocal signals from MCs stimulated via ST2 enhance IL-33 production by fibroblasts, constituting a MC-fibroblast amplification loop.
Beyond IL-1β, we found that IL-33 priming of MCs enhanced FcγRIII-mediated release of multiple other mediators, including CXCL2. The CXCL2 receptor CXCR2 is required for the development of full pathology in K/BxN arthritis, and in particular for neutrophil recruitment [29], [41], [43], [47]. Our findings therefore develop further our understanding of mechanisms by which MCs help recruit neutrophils to the inflamed joint [33].
These results are consistent with published data showing that IL-33 amplifies mediator production resulting from stimulation of MCs via IgE, since both FcγRIII and FcεRI signal via a common Fc receptor γ chain [24], [25]. Further, our results confirm that IL-33 can itself drive MC production of a range of mediators, including the unusual combination of IL-6 and IL-2 that could potentially contribute to the subversion of regulatory T cell function (Figure 2D) [48]. Such direct stimulation of MCs may contribute to inflammatory arthritis, particularly in chronic synovitis, when the population of synovial MCs is often markedly expanded [11].
However, our results suggest an alternate mechanism by which IL-33 contributes to acute MC activation in IgG-mediated arthritis. In K/BxN arthritis, the MC-dependent “flare” begins within minutes of serum administration, a timeframe probably too short for de novo IL-33 synthesis. Rather, consistent with published results demonstrating the key role of FcγRIII in synovial MC activation [33], [35], our data suggest that constitutive signals mediated via IL-33 promote immune complex responsiveness of synovial MCs, defining therefore a new model for a permissive role of IL-33 in MC-dependent immune complex disease (Figure 5). Whereas IL-33 pre-incubation induces accumulation of mRNA (and to a lesser extent intracellular protein) for key pro-inflammatory cytokines whose production by subsequent FcγRIII ligation is markedly enhanced, we hypothesize that such “pre-loading” of MC by IL-33 represents an important component of the priming mechanism, though other factors may also be involved.
Our results also expand appreciation of the integral relationship between MCs and fibroblasts. We previously demonstrated a profound effect of fibroblasts on the development of MCs [5], [6], [26]. The current work builds upon these studies, showing that IL-33 is a key mediator by which fibroblasts prime MCs for activation by IgG immune complexes. Given the known anatomic and functional associations of synovial MC with fibroblasts, these cells represent the most likely source of IL-33 in the joint, a possibility modeled by our in vitro co-culture system. However, endothelial cells or other IL-33-producing lineages, including MCs themselves, could potentially fulfill the same role.
While our in vitro findings correspond well to the expected activity of MCs in arthritis, it is possible that our system fails to model all aspects of the in vivo biology. In particular, we observed evidence for reduced MC activation in ST2−/− animals exposed to K/BxN IgG, manifested as reduced flare magnitude. This result supports the observation that MC degranulation (observed at day 4 tissue harvest) is impaired in ST2−/− mice administered K/BxN serum [31]. However, consistent with most published reports, we found no in vitro effect of IL-33 on degranulation of cultured MCs, either alone or together with FcγRIII ligation [13], [25]. Further, whereas exposure of WT MCs to IL-33 enabled these cells to bypass inhibition by FcγRII with respect to production of IL-6, we could not induce FcγRIII-mediated degranulation or IL-1β production (data not shown). These observations may reflect phenotypic variance between cultured MCs and those that have matured within synovial tissues, or potentially the absence of a required cofactor, given the recent finding that animals deficient in the receptor for IL-4 fail to demonstrate tissue MC degranulation induced by repeated injections of recombinant IL-33 [49]. Alternately, it may be that the initial MC activation step that provides the “jump start” to arthritis does not obligatorily involve degranulation. Indeed, the precise mechanisms mediating the flare remain to be defined, and are known to involve neutrophils as well as MCs, such that the flare is unlikely to simply represent local anaphylaxis-like release of MC granule contents [32], [50].
Our results do not define the pathways by which fibroblasts produce and release IL-33. Indeed, this remains an area of substantial uncertainty within IL-33 biology [34]. Like most other members of the IL-1 family, IL-33 does not possess a signal peptide permitting conventional secretion. Since IL-33 is inactivated by caspases, it has been suggested that it may represent a “alarmin,” liberated during necrosis but not apoptosis [51]. Indeed, some degree of necrosis was detectable in our cultures by lactate dehydrogenase release (data not shown), though whether such necrosis is relevant to our in vitro observation, or indeed to the in vivo arthritis phenotype, is unknown. Interestingly, we recently showed that cardiac fibroblasts can release IL-33 upon mechanical stretch, providing one potential mechanism by which fibroblasts within a moving joint might release IL-33, thereby priming MCs [52]. However, this mechanism would not have been expected to be operative in our static culture system.
In summary, our results show that IL-33 has the previously unrecognized potential to enhance MC responses to FcγRIII ligation. Our previous studies have demonstrated that MCs activated via FcγRIII can “jump start” synovial inflammation, at least in part via the pro-inflammatory cytokine IL-1β [35]. Recent in vivo studies, confirmed here, have implicated MC expression of ST2 in arthritis [21], [31]. Our current results link these observations together, showing that priming of MCs via IL-33 potentiates their activation via FcγRIII, resulting in markedly enhanced production of IL-1β, IL-6, and other mediators (Figure 5). Since immune complexes deposited within synovial tissue are a hallmark of rheumatoid arthritis [53], our results suggest that blockade of the IL-33/ST2 axis could benefit from a multiplier effect, dampening cell activation resulting not only from IL-33 itself but also from mechanisms amplified by this cytokine, including Fc receptors ligation in MCs. These results therefore further support IL-33 as a potential candidate for therapeutic inhibition in arthritis.
Acknowledgments
We thank Dr. Michael B. Brenner (Brigham and Women’s Hospital, Boston, MA) for providing the K/BxN mouse serum used in our arthritis experiments; Nicholas Calderone, Blair Chaletzky and Sarah Ameri for expert technical support; Dr. Pushpalatha Jayaraman (Brigham and Women’s Hospital, Boston, MA) for assistance with the lactate dehydrogenase assay; and Dr. Jing Cui (Brigham and Women’s Hospital, Boston, MA) for statistical expertise.
Author Contributions
Conceived and designed the experiments: SK JXW PAN. Performed the experiments: SK JXW RS NF HH PAN. Analyzed the data: SK JXW RLS PAN. Contributed reagents/materials/analysis tools: RTL. Wrote the paper: SK JXW PAN.
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