TY - JOUR T1 - Spontaneous Reperfusion after In Situ Thromboembolic Stroke in Mice A1 - Durand, Anne A1 - Chauveau, Fabien A1 - Cho, Tae-Hee A1 - Bolbos, Radu A1 - Langlois, Jean-Baptiste A1 - Hermitte, Laure A1 - Wiart, Marlène A1 - Berthezène, Yves A1 - Nighoghossian, Norbert Y1 - 2012/11/16 N2 - Injection of thrombin into the middle cerebral artery (MCA) of mice has been proposed as a new model of thromboembolic stroke. The present study used sequential multiparametric Magnetic Resonance Imaging (MRI), including Magnetic Resonance Angiography (MRA), Diffusion-Weighted Imaging (DWI) and Perfusion-Weighted Imaging (PWI), to document MCA occlusion, PWI-DWI mismatch, and lesion development. In the first experiment, complete MCA occlusion and reproducible hypoperfusion were obtained in 85% of animals during the first hour after stroke onset. In the second experiment, 80% of animals showed partial to complete reperfusion during a three-hour follow-up. Spontaneous reperfusion thus contributed to the variability in ischemic volume in this model. The study confirmed the value of the model for evaluating new thrombolytic treatments, but calls for extended MRI follow-up at the acute stage in therapeutic studies. JF - PLOS ONE JA - PLOS ONE VL - 7 IS - 11 UR - https://doi.org/10.1371/journal.pone.0050083 SP - e50083 EP - PB - Public Library of Science M3 - doi:10.1371/journal.pone.0050083 ER -