TY - JOUR T1 - Specific Association of Lectin LecB with the Surface of Pseudomonas aeruginosa: Role of Outer Membrane Protein OprF A1 - Funken, Horst A1 - Bartels, Kai-Malte A1 - Wilhelm, Susanne A1 - Brocker, Melanie A1 - Bott, Michael A1 - Bains, Manjeet A1 - Hancock, Robert E. W. A1 - Rosenau, Frank A1 - Jaeger, Karl-Erich Y1 - 2012/10/08 N2 - The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF. JF - PLOS ONE JA - PLOS ONE VL - 7 IS - 10 UR - https://doi.org/10.1371/journal.pone.0046857 SP - e46857 EP - PB - Public Library of Science M3 - doi:10.1371/journal.pone.0046857 ER -