TY - JOUR T1 - Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging A1 - Kelly, Kimberly A. A1 - Carson, Jonathan A1 - McCarthy, Jason R. A1 - Weissleder, Ralph Y1 - 2007/07/25 N2 - BackgroundProbes that allow site-specific protein labeling have become critical tools for visualizing biological processes.MethodsHere we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence (“IQ-tag”) allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging.SignificanceThe described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development. JF - PLOS ONE JA - PLOS ONE VL - 2 IS - 7 UR - https://doi.org/10.1371/journal.pone.0000665 SP - e665 EP - PB - Public Library of Science M3 - doi:10.1371/journal.pone.0000665 ER -