@article{10.1371/journal.pone.0000665, doi = {10.1371/journal.pone.0000665}, author = {Kelly, Kimberly A. AND Carson, Jonathan AND McCarthy, Jason R. AND Weissleder, Ralph}, journal = {PLOS ONE}, publisher = {Public Library of Science}, title = {Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging}, year = {2007}, month = {07}, volume = {2}, url = {https://doi.org/10.1371/journal.pone.0000665}, pages = {1-9}, abstract = {BackgroundProbes that allow site-specific protein labeling have become critical tools for visualizing biological processes.MethodsHere we used phage display to identify a novel peptide sequence with nanomolar affinity for near infrared (NIR) (benz)indolium fluorochromes. The developed peptide sequence (“IQ-tag”) allows detection of NIR dyes in a wide range of assays including ELISA, flow cytometry, high throughput screens, microscopy, and optical in vivo imaging.SignificanceThe described method is expected to have broad utility in numerous applications, namely site-specific protein imaging, target identification, cell tracking, and drug development.}, number = {7}, }