CLANS program and manual link: http://bioinfoserver.rsbs.anu.edu.au/downloads/clans/ program: clans.jar manual: CLANS_tutorial.pdf (if you use this program for a publication, please cite: Frickey T, Lupas A (2004) CLANS: a Java application for visualizing protein families based on pairwise similarity. Bioinformatics 20: 3702-4.) CLANS-save-files for the sequence-similarity maps depicted in Figures 3-9 and the Hox15, NOB-1 and EGL-5 analysis are available at (these are bigger files than the sequence files, but provide pre-clustered and labelled maps): http://bioinfoserver.rsbs.anu.edu.au/downloads/hox_classification/ to open the CLANS save files: - download the Clans program and CLANS-save-files of interest to a chosen directory - open a terminal / command line (see tutorial) - go to the directory in which the clans.jar and Clans-save-files are located - type "java -jar -Xmx3000m clans.jar filename" (for bigger files you may need up to 12 GB of RAM, in that case replace the 3000 with a 12000 in the -Xmx setting) Alignment files can be viewed with AlnEdit http://bioinfoserver.rsbs.anu.edu.au/programs/alnedit/