I. Reducing CLV3 expression
Conditional loss-of-function of CLV3 results in an expansion of the OC and the SCD. In addition, the OC is shifted towards the tip due to a loss of negative feedback by CLV3. When CLV3 expression levels are only mildly reduced, a graded response was observed: SCD and OC enlarge, but the location of the OC is not affected due to active CLV3 signalling. A typical time course of a simulation for a conditional reduction in CLV3 expression is shown in FigS1A. Interestingly, OC size remained unaffected for a wide range of endogenous CLV3 expression levels (Fig. S1B), indicating a partial uncoupling of OC and SCD sizes.