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Open Access

Peer-reviewed

Research Article

De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth

Michael A. Fisher,

Current address: Energy Biosciences Institute, University of California, Berkeley, California, United States of America

Affiliation Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
⨯
Kara L. McKinley,

Current address: Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Affiliation Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
⨯
Luke H. Bradley,

Current address: Departments of Anatomy and Neurobiology, Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America

Affiliation Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
⨯
Sara R. Viola,

Current address: Columbia University Medical Center, New York, New York, United States of America

Affiliation Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
⨯
Michael H. Hecht

* E-mail: hecht@princeton.edu

Affiliation Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America
⨯

De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth

Michael A. Fisher,
Kara L. McKinley,
Luke H. Bradley,
Sara R. Viola,
Michael H. Hecht

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Published: January 4, 2011
https://doi.org/10.1371/journal.pone.0015364

Reader Comments (7)

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30 Apr 2013
19:41 GMT
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IS IT POSSIBLE?? Posted by aashami on 30 Apr 2013 at 19:41 GMT
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30 Apr 2013
10:01 GMT
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What is life ? Posted by aashami on 30 Apr 2013 at 10:01 GMT
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29 Apr 2013
18:46 GMT
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PATHWAYS Posted by aashami on 29 Apr 2013 at 18:46 GMT
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20 Feb 2011
09:44 GMT
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is that surprising? Posted by Vincent_Danos on 19 Feb 2011 at 17:28 GMT
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16 Jan 2011
04:19 GMT
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Very interesting method Posted by Slava on 16 Jan 2011 at 04:19 GMT
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06 Jan 2011
19:51 GMT
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Very interesting! Posted by Derya on 06 Jan 2011 at 19:51 GMT

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Cell growth
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Protein folding
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Plasmid construction
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Protein expression
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