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Research Article

Elicitation of Both Anti HIV-1 Env Humoral and Cellular Immunities by Replicating Vaccinia Prime Sendai Virus Boost Regimen and Boosting by CD40Lm

  • Xianfeng Zhang,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Tomoyoshi Sobue,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Mao Isshiki,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Shun-ichi Makino,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Makoto Inoue,

    Affiliation: DNAVEC Corporation, Techno Park Oho, Tsukuba, Ibaraki, Japan

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  • Kazunori Kato,

    Affiliation: Department of BioMedical Engineering, Toyo University, Kawagoe, Saitama, Japan

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  • Tatsuo Shioda,

    Affiliation: Department of Viral Infection, Research Institute for Microbial Disease, Osaka University, Yamada-oka, Suita-shi, Osaka, Japan

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  • Takashi Ohashi,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Hirotaka Sato,

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Jun Komano,

    Affiliation: Division of Virology, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health, Nakamichi Higashinari-ku, Osaka, Japan

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  • Hideji Hanabusa,

    Affiliation: Ogikubo Hospital, Ogikubo, Suginami, Tokyo, Japan

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  • Hisatoshi Shida mail

    hshida@igm.hokudai.ac.jp

    Affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan

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  • Published: December 07, 2012
  • DOI: 10.1371/journal.pone.0051633

About the Authors

Xianfeng Zhang, Tomoyoshi Sobue, Mao Isshiki, Shun-ichi Makino, Takashi Ohashi, Hirotaka Sato, Hisatoshi Shida
Institute for Genetic Medicine, Hokkaido University, Kita-ku, Sapporo, Japan
Makoto Inoue
DNAVEC Corporation, Techno Park Oho, Tsukuba, Ibaraki, Japan
Kazunori Kato
Department of BioMedical Engineering, Toyo University, Kawagoe, Saitama, Japan
Tatsuo Shioda
Department of Viral Infection, Research Institute for Microbial Disease, Osaka University, Yamada-oka, Suita-shi, Osaka, Japan
Jun Komano
Division of Virology, Department of Infectious Diseases, Osaka Prefectural Institute of Public Health, Nakamichi Higashinari-ku, Osaka, Japan
Hideji Hanabusa
Ogikubo Hospital, Ogikubo, Suginami, Tokyo, Japan

Corresponding Author

Email: hshida@igm.hokudai.ac.jp

Competing Interests

The authors declare one author, M. Inoue, has an affiliation in DNAVEC Corporation. Although DNAVEC, a profit company, has developed and is preparing for marketing the Sendai Virus Vector (SeV Vector), it has not financially supported this study at all. This study has been exclusively supported by the grants (No: 21390135) of Ministry of Education, Culture, Sports, Science and Technology and that (No: SAB4861) of Human Health Foundation. Thus, participation of Makoto Inoue, a member of DNAVEC, does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: XZ H. Shida. Performed the experiments: XZ T. Sobue M. Isshiki SM H. Sato. Analyzed the data: XZ T. Sobue M. Isshiki SM H. Shida. Contributed reagents/materials/analysis tools: M. Inoue KK T. Shioda TO JK HH. Wrote the paper: XZ H. Shida.