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Research Article

Porcine Model of Hemophilia A

  • Yuji Kashiwakura equal contributor,

    equal contributor Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Jun Mimuro equal contributor mail,

    equal contributor Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto

    mimuro-j@jichi.ac.jp

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

    X
  • Akira Onishi equal contributor,

    equal contributor Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto

    Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan

    X
  • Masaki Iwamoto equal contributor,

    equal contributor Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto

    Affiliations: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan, Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan

    X
  • Seiji Madoiwa,

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Daiichiro Fuchimoto,

    Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan

    X
  • Shunichi Suzuki,

    Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan

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  • Misae Suzuki,

    Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan

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  • Shoichiro Sembon,

    Affiliation: Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan

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  • Akira Ishiwata,

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Atsushi Yasumoto,

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Asuka Sakata,

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Tsukasa Ohmori,

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Michiko Hashimoto,

    Affiliation: Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan

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  • Satoko Yazaki,

    Affiliation: Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan

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  • Yoichi Sakata

    Affiliation: Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan

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  • Published: November 28, 2012
  • DOI: 10.1371/journal.pone.0049450

About the Authors

Yuji Kashiwakura, Jun Mimuro, Seiji Madoiwa, Akira Ishiwata, Atsushi Yasumoto, Asuka Sakata, Tsukasa Ohmori, Yoichi Sakata
Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
Akira Onishi, Masaki Iwamoto, Daiichiro Fuchimoto, Shunichi Suzuki, Misae Suzuki, Shoichiro Sembon
Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
Masaki Iwamoto, Michiko Hashimoto, Satoko Yazaki
Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan

Corresponding Author

Email: mimuro-j@jichi.ac.jp

Competing Interests

MI, MH, and SY are employees of Prim Tech Ltd., the developer of Piezo micromanipulators for nuclear transfer, and are collaborative with National Institute of Agrobiological Sciences and Jichi Meidical University for basic research. Other authors declare no conflict of interest. This does not alter the authors adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Wrote the paper: JM. Senior investigator and supervised this study: YS. Acted as a senior investigator, planned, generated constructs for F8 gene targeting, and conducted the study: JM. Postdoctoral fellow, conducted most of the F8 targeting work and injected human FVIII into a cloned piglet: YK. Performed nuclear transfer of targeted cells to oocytes and transplantation of the oocytes, and managed care of cloned pigs: AO MI. Injected human FVIII into a cloned piglet on day 1 after birth: SM. Cared for cloned piglets and did pathological examination of cloned piglets: DF SS MS SS MH SY. Performed cell culture experiments: AI AY AS TO.