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Research Article

A Human Blood-Brain Barrier Transcytosis Assay Reveals Antibody Transcytosis Influenced by pH-Dependent Receptor Binding

  • Hadassah Sade,

    Affiliation: Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Penzberg, Germany

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  • Claudia Baumgartner,

    Affiliation: Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Penzberg, Germany

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  • Adrian Hugenmatter,

    Affiliation: Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Schlieren, Switzerland

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  • Ekkehard Moessner,

    Affiliation: Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Schlieren, Switzerland

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  • Per-Ola Freskgård,

    Affiliation: Neuroscience Discovery and Translation Area, Pharma Research and Early Development (pRED), F. Hoffmann-La Roche, Basel, Switzerland

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  • Jens Niewoehner mail

    jens.niewoehner@roche.com

    Affiliation: Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Penzberg, Germany

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  • Published: April 30, 2014
  • DOI: 10.1371/journal.pone.0096340

About the Authors

Hadassah Sade, Claudia Baumgartner, Jens Niewoehner
Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Penzberg, Germany
Adrian Hugenmatter, Ekkehard Moessner
Large Molecule Research, Pharma Research and Early Development (pRED), Roche, Schlieren, Switzerland
Per-Ola Freskgård
Neuroscience Discovery and Translation Area, Pharma Research and Early Development (pRED), F. Hoffmann-La Roche, Basel, Switzerland

Corresponding Author

Email: jens.niewoehner@roche.com

Competing Interests

The authors have read the journal's policy and have the following conflicts: All authors are paid employees of Roche affiliates, whose company funded this study. There are no products in development or marketed products to declare. Part of the data described in the paper are included in the patent application: "Method and Constructs for the pH Dependent Passage of the Blood-brain-barrier", US20120282176. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: JN HS AH EM POF. Performed the experiments: HS CB. Analyzed the data: HS JN CB. Wrote the paper: HS JN.