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Research Article

Simple and Efficient Methods for Enrichment and Isolation of Endonuclease Modified Cells

  • Branden S. Moriarity,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Eric P. Rahrmann,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Dominic A. Beckmann,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Caitlin B. Conboy,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Adrienne L. Watson,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Daniel F. Carlson,

    Affiliations: Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Animal Science, University of Minnesota, Minneapolis, Minnesota, United States of America, Recombinetics, Inc., Saint Paul, Minnesota, United States of America

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  • Erik R. Olson,

    Affiliation: Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

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  • Kendra A. Hyland,

    Affiliation: Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

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  • Scott C. Fahrenkrug,

    Affiliations: Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Animal Science, University of Minnesota, Minneapolis, Minnesota, United States of America, Recombinetics, Inc., Saint Paul, Minnesota, United States of America

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  • R. Scott McIvor,

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

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  • David A. Largaespada mail

    larga002@umn.edu

    Affiliations: Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America, Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America, Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, United States of America

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  • Published: May 05, 2014
  • DOI: 10.1371/journal.pone.0096114

About the Authors

Branden S. Moriarity, Eric P. Rahrmann, Dominic A. Beckmann, Caitlin B. Conboy, Adrienne L. Watson, R. Scott McIvor, David A. Largaespada
Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America
Branden S. Moriarity, Eric P. Rahrmann, Dominic A. Beckmann, Daniel F. Carlson, Scott C. Fahrenkrug, R. Scott McIvor, David A. Largaespada
Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America
Branden S. Moriarity, Eric P. Rahrmann, Dominic A. Beckmann, Caitlin B. Conboy, Adrienne L. Watson, David A. Largaespada
Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America
Erik R. Olson, Kendra A. Hyland, R. Scott McIvor, David A. Largaespada
Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America
David A. Largaespada
Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, United States of America
Daniel F. Carlson, Scott C. Fahrenkrug
Department of Animal Science, University of Minnesota, Minneapolis, Minnesota, United States of America
Daniel F. Carlson, Scott C. Fahrenkrug
Recombinetics, Inc., Saint Paul, Minnesota, United States of America

Corresponding Author

Email: larga002@umn.edu

Competing Interests

R.S.M is a founder and managing employee of Discovery Genomics, Inc. (DGI), including stock and options. K.H. and E.O. are employees of DGI with stock options. D.A.L. is a founder of DGI with stock. D.A.L. is a founder of NeoClone Biotechnology, Inc. with stock. D.F.C and S.F are currently an employee of and CEO, respectfully, of Recombinetics, Inc. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: BSM DAL RSM. Performed the experiments: BSM DAB EPR CBC ALW KAH. Analyzed the data: BSM DAB EPR. Contributed reagents/materials/analysis tools: DFC SCF ERO. Wrote the paper: BSM.