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Research Article

Enhanced Reliability and Accuracy for Field Deployable Bioforensic Detection and Discrimination of Xylella fastidiosa subsp. pauca, Causal Agent of Citrus Variegated Chlorosis Using Razor Ex Technology and TaqMan Quantitative PCR

  • Ping Ouyang equal contributor,

    equal contributor Contributed equally to this work with: Ping Ouyang, Mohammad Arif

    Affiliations: National Institute for Microbial Forensics & Food and Agricultural Biosecurity, Oklahoma State University, Stillwater, Oklahoma, United States of America, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, United States of America

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  • Mohammad Arif equal contributor,

    equal contributor Contributed equally to this work with: Ping Ouyang, Mohammad Arif

    Affiliations: National Institute for Microbial Forensics & Food and Agricultural Biosecurity, Oklahoma State University, Stillwater, Oklahoma, United States of America, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, United States of America

    Current address: Department of Plant Pathology, Kansas State University, Manhattan, Kansas, United States of America

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  • Jacqueline Fletcher mail,

    * Email: Jacqueline.fletcher@okstate.edu (JF); ochoaco@okstate.edu (MOC)

    Affiliations: National Institute for Microbial Forensics & Food and Agricultural Biosecurity, Oklahoma State University, Stillwater, Oklahoma, United States of America, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, United States of America

    X
  • Ulrich Melcher,

    Affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, United States of America

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  • Francisco Manuel Ochoa Corona mail

    * Email: Jacqueline.fletcher@okstate.edu (JF); ochoaco@okstate.edu (MOC)

    Affiliations: National Institute for Microbial Forensics & Food and Agricultural Biosecurity, Oklahoma State University, Stillwater, Oklahoma, United States of America, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, United States of America

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  • Published: November 29, 2013
  • DOI: 10.1371/journal.pone.0081647

About the Authors

Ping Ouyang, Mohammad Arif, Jacqueline Fletcher, Francisco Manuel Ochoa Corona
National Institute for Microbial Forensics & Food and Agricultural Biosecurity, Oklahoma State University, Stillwater, Oklahoma, United States of America
Ping Ouyang, Mohammad Arif, Jacqueline Fletcher, Francisco Manuel Ochoa Corona
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, United States of America
Ulrich Melcher
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MA FMOC JF UM. Performed the experiments: PO MA. Analyzed the data: MA. Contributed reagents/materials/analysis tools: JF FMOC. Wrote the manuscript: PO MA JF UM FMOC.