About the Authors

Sílvia Fontenete

Affiliations LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark, ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal

Nuno Guimarães

Affiliations LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark

Marina Leite

Affiliation IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

Céu Figueiredo

Affiliations IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal, FMUP, Faculty of Medicine of the University of Porto, Porto, Portugal

Jesper Wengel

Affiliation Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark

Nuno Filipe Azevedo

nazevedo@fe.up.pt

Affiliation LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal

Competing Interests

Jesper Wengel is cofounder of RiboTask ApS which offer LNA/2’-OMe-RNA probes for RNA targeting. Nuno Filipe Azevedo is cofounder of Biomode SA which develops molecular methods for the rapid detection of microorganisms. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: SF NG CF JW NFA. Performed the experiments: SF NG ML. Analyzed the data: SF NG JW ML NFA. Contributed reagents/materials/analysis tools: SF NG ML NFA JW CF. Wrote the manuscript: SF NG NFA.