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Research Article

Application of Genotyping-by-Sequencing on Semiconductor Sequencing Platforms: A Comparison of Genetic and Reference-Based Marker Ordering in Barley

  • Martin Mascher,

    Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

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  • Shuangye Wu,

    Affiliation: Department of Agronomy, Kansas State University, Manhattan, Kansas, United States of America

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  • Paul St. Amand,

    Affiliation: United States Department of Agriculture, Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, Kansas, United States of America

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  • Nils Stein,

    Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

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  • Jesse Poland mail

    jpoland@ksu.edu

    Affiliations: Department of Agronomy, Kansas State University, Manhattan, Kansas, United States of America, United States Department of Agriculture, Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, Kansas, United States of America

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  • Published: October 03, 2013
  • DOI: 10.1371/journal.pone.0076925

About the Authors

Martin Mascher, Nils Stein
Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
Shuangye Wu, Jesse Poland
Department of Agronomy, Kansas State University, Manhattan, Kansas, United States of America
Paul St. Amand, Jesse Poland
United States Department of Agriculture, Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, Kansas, United States of America

Corresponding Author

Email: jpoland@ksu.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JP NS. Performed the experiments: SW PSt. Analyzed the data: MM JP. Wrote the manuscript: JP NS MM PSt.