About the Authors

Alison J. Basile

Ajj1@cdc.gov

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Kalanthe Horiuchi

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Amanda J. Panella

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Janeen Laven

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Olga Kosoy

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Robert S. Lanciotti

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Neeraja Venkateswaran

Current address: Tetracore, Inc, Rockville, Maryland, United States of America

Affiliation Radix Biosolutions, Georgetown, Texas, United States of America

Brad J. Biggerstaff

Affiliation Division of Vector-Borne Diseases, Centers for Disease Control, Fort Collins, Colorado, United States of America

Competing Interests

a) Authors Alison Basile and Brad Biggerstaff are named inventors on US patent 7,933,721 and Alison Basile is the named inventor on US patent 8,433,523. These patents are related to precursor methods for the one described in this manuscript. b) The CDC has an informal affiliation with Radix BioSolutions such that the CDC provides monoclonal antibodies for the purposes of coupling to microspheres at Radix for precursor tests related to that described in the manuscript at hand. Radix makes these available to State Health laboratories that use the CDC microsphere tests. There is no exchange of money between CDC and Radix in this arrangement; it serves to relieve the CDC for manufacturing and shipping these reagents and allows for standardized reagents. Co-author Neeraja Venkatsewaran was a former employee of Radix (now at Tetracore with whom the authors' have no affiliation) and agreed to make control microspheres for the test as a favor to the CDC. These declarations do not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: AJB RL. Performed the experiments: AJB AJP JL OK. Analyzed the data: AJB KH BJB. Contributed reagents/materials/analysis tools: NV. Wrote the manuscript: AJB BJB.