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Research Article

SNP Detection in mRNA in Living Cells Using Allele Specific FRET Probes

  • Liya Dahan,

    Affiliations: Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel, Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel

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  • Lingyan Huang,

    Affiliation: Integrated DNA Technologies, Coralville, Iowa, United States of America

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  • Ranit Kedmi,

    Affiliations: Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel, Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel

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  • Mark A. Behlke mail,

    mbehlke@idtdna.com (MAB); peer@tauex.tau.ac.il (DP)

    Affiliation: Integrated DNA Technologies, Coralville, Iowa, United States of America

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  • Dan Peer mail

    mbehlke@idtdna.com (MAB); peer@tauex.tau.ac.il (DP)

    Affiliations: Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel, Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel

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  • Published: September 09, 2013
  • DOI: 10.1371/journal.pone.0072389

About the Authors

Liya Dahan, Ranit Kedmi, Dan Peer
Department of Cell Research and Immunology, Tel Aviv University, Tel Aviv, Israel
Liya Dahan, Ranit Kedmi, Dan Peer
Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel
Lingyan Huang, Mark A. Behlke
Integrated DNA Technologies, Coralville, Iowa, United States of America

Corresponding Authors

Competing Interests

MAB and LH are employees of IDT; DP has financial interest in Quiet Therapeutics. The remaining authors declare no competing financial interests. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: DP MAB. Performed the experiments: LD RK LH. Analyzed the data: LD MAB DP. Contributed reagents/materials/analysis tools: LH MAB. Wrote the paper: LD MAB DP.