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Research Article

Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents

  • Susanne Meyer equal contributor,

    equal contributor Contributed equally to this work with: Susanne Meyer, John P. Maufort

    Affiliation: Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America

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  • John P. Maufort equal contributor,

    equal contributor Contributed equally to this work with: Susanne Meyer, John P. Maufort

    Affiliation: Morgridge Institute for Research, Madison, Wisconsin, United States of America

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  • Jeff Nie,

    Affiliation: Morgridge Institute for Research, Madison, Wisconsin, United States of America

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  • Ron Stewart,

    Affiliation: Morgridge Institute for Research, Madison, Wisconsin, United States of America

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  • Brian E. McIntosh,

    Affiliation: Morgridge Institute for Research, Madison, Wisconsin, United States of America

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  • Lisa R. Conti,

    Affiliation: Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America

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  • Kareem M. Ahmad,

    Affiliation: Interdepartmental Program in Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California, United States of America

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  • H. Tom Soh,

    Affiliations: Department of Materials, University of California Santa Barbara, Santa Barbara, California, United States of America, Department of Mechanical Engineering, University of California Santa Barbara, Santa Barbara, California, United States of America

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  • James A. Thomson mail

    jthomson@morgridgeinstitute.org

    Affiliations: Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America, Morgridge Institute for Research, Madison, Wisconsin, United States of America, Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America

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  • Published: August 13, 2013
  • DOI: 10.1371/journal.pone.0071798

About the Authors

Susanne Meyer, Lisa R. Conti, James A. Thomson
Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America
John P. Maufort, Jeff Nie, Ron Stewart, Brian E. McIntosh, James A. Thomson
Morgridge Institute for Research, Madison, Wisconsin, United States of America
James A. Thomson
Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America
H. Tom Soh
Department of Materials, University of California Santa Barbara, Santa Barbara, California, United States of America
H. Tom Soh
Department of Mechanical Engineering, University of California Santa Barbara, Santa Barbara, California, United States of America
Kareem M. Ahmad
Interdepartmental Program in Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, California, United States of America

Corresponding Author

Email: jthomson@morgridgeinstitute.org

Competing Interests

JAT is a founder, stockowner, consultant and board member of Cellular Dynamics International (CDI). He also serves as scientific advisor to and has financial interests in Tactics II Stem Cell Ventures. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: SM JPM JAT. Performed the experiments: SM JPM BM. Analyzed the data: SM JMP JAT. Contributed reagents/materials/analysis tools: RS JN KMA. Wrote the paper: SM LC HTS JAT.