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Research Article

Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface

  • Andrew C. Payne equal contributor,

    equal contributor Contributed equally to this work with: Andrew C. Payne, Michael Andregg

    Affiliations: Wyss Institute, Harvard University, Boston, Massachusetts, United States of America, Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Michael Andregg equal contributor,

    equal contributor Contributed equally to this work with: Andrew C. Payne, Michael Andregg

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Kent Kemmish,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Mark Hamalainen,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Charlotte Bowell,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Andrew Bleloch,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

    X
  • Nathan Klejwa,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

    X
  • Wolfgang Lehrach,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Ken Schatz,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Heather Stark,

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Adam Marblestone,

    Affiliation: Biophysics Program and Wyss Institute, Harvard University, Boston, Massachusetts, United States of America

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  • George Church mail,

    gchurch@genetics.med.harvard.edu (GC); csown@voxa.co (CO)

    Affiliations: Biophysics Program and Wyss Institute, Harvard University, Boston, Massachusetts, United States of America, Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Christopher S. Own mail,

    gchurch@genetics.med.harvard.edu (GC); csown@voxa.co (CO)

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • William Andregg

    Affiliation: Halcyon Molecular, Inc., Redwood City, California, United States of America

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  • Published: July 31, 2013
  • DOI: 10.1371/journal.pone.0069058

About the Authors

Andrew C. Payne
Wyss Institute, Harvard University, Boston, Massachusetts, United States of America
Andrew C. Payne, Michael Andregg, Kent Kemmish, Mark Hamalainen, Charlotte Bowell, Andrew Bleloch, Nathan Klejwa, Wolfgang Lehrach, Ken Schatz, Heather Stark, Christopher S. Own, William Andregg
Halcyon Molecular, Inc., Redwood City, California, United States of America
Adam Marblestone, George Church
Biophysics Program and Wyss Institute, Harvard University, Boston, Massachusetts, United States of America
George Church
Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

Corresponding Authors

Competing Interests

Two US patents has been filed on related work by authors of this manuscript: Sequencing Nucleic Acid Polymers with Electron Microscopy, inventors: William Andregg, Michael Andregg, US patent no. 8,153,438; Scanning Transmission Electron Microscopy, inventors: Christopher Su-Yan Own, William Andregg, Michael Andregg, Application number: 13/303,121 (pending publication), Filing date: 11/22/2011. Several of the authors are employed by Halcyon Molecular Inc. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: AP MA KK MH AB KS AM GMC CSO WA. Performed the experiments: AP MA KK CB HS CSO. Analyzed the data: AP MH WL CSO. Contributed reagents/materials/analysis tools: AP NK WL KS HS GMC CSO. Wrote the paper: AP AM CSO. Supervised the experiments: GMC AM CSO. Obtained funding: GMC CSO WA MA KK.