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Research Article

Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial

  • Delia Bethell mail,

    delia.bethell@afrims.org

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • David Saunders,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Anan Jongkaewwattana,

    Affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Bangkok, Thailand

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  • Jarin Kramyu,

    Affiliation: Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Bangkok, Thailand

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  • Arunee Thitithayanont,

    Affiliation: Faculty of Science, Mahidol University, Bangkok, Thailand

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  • Suwimon Wiboon-ut,

    Affiliation: Faculty of Science, Mahidol University, Bangkok, Thailand

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  • Kosol Yongvanitchit,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Amporn Limsalakpetch,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Utaiwan Kum-Arb,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Nichapat Uthaimongkol,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Jean Michel Garcia,

    Affiliation: University of Hong Kong-Pasteur Research Center, Hong Kong Special Administrative Region, China

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  • Ans E. Timmermans,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Malik Peiris,

    Affiliation: University of Hong Kong-Pasteur Research Center, Hong Kong Special Administrative Region, China

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  • Stephen Thomas,

    Affiliation: Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Anneke Engering,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Richard G. Jarman,

    Affiliation: Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Duangrat Mongkolsirichaikul,

    Affiliation: Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Carl Mason,

    Affiliation: Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Nuanpan Khemnu,

    Affiliation: Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Stuart D. Tyner,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Mark M. Fukuda,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Douglas S. Walsh,

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Sathit Pichyangkul

    Affiliation: Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

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  • Published: March 26, 2013
  • DOI: 10.1371/journal.pone.0059674

About the Authors

Delia Bethell, David Saunders, Kosol Yongvanitchit, Amporn Limsalakpetch, Utaiwan Kum-Arb, Nichapat Uthaimongkol, Ans E. Timmermans, Anneke Engering, Stuart D. Tyner, Mark M. Fukuda, Douglas S. Walsh, Sathit Pichyangkul
Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Anan Jongkaewwattana, Jarin Kramyu
Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Bangkok, Thailand
Arunee Thitithayanont, Suwimon Wiboon-ut
Faculty of Science, Mahidol University, Bangkok, Thailand
Jean Michel Garcia, Malik Peiris
University of Hong Kong-Pasteur Research Center, Hong Kong Special Administrative Region, China
Stephen Thomas, Richard G. Jarman, Duangrat Mongkolsirichaikul
Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Carl Mason, Nuanpan Khemnu
Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

Corresponding Author

Email: delia.bethell@afrims.org

Competing Interests

The authors declare they have no competing interests. This study was supported by MedImmune, which also provided FluMist Seasonal Trivalent Live Attenuated Intranasal Vaccine for use in the trial. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. There are no patents, products in development or marketed products to declare.

Author Contributions

Conceived and designed the experiments: DB DS AE SDT MF SP. Performed the experiments: DB DS AJ JK AT SW KY AL UK NU JMG AT MP ST RGJ DM NK SDT DSW SP. Analyzed the data: DB DS KY AL NU MMF DSW SP. Contributed reagents/materials/analysis tools: AJ JK AT SW JMG MP CM. Wrote the paper: DB DS AT JMG AT ST AE RGJ SDT MMF DSW SP.