Advertisement
Research Article

Robust Detection of Rare Species Using Environmental DNA: The Importance of Primer Specificity

  • Taylor M. Wilcox mail,

    taylor.wilcox@umontana.edu

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

    X
  • Kevin S. McKelvey,

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

    X
  • Michael K. Young,

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

    X
  • Stephen F. Jane,

    Affiliation: Department of Environmental Conservation, University of Massachusetts, Amherst, Massachusetts, United States of America

    X
  • Winsor H. Lowe,

    Affiliation: Division of Biological Sciences, University of Montana, Missoula, Montana, United States of America

    X
  • Andrew R. Whiteley,

    Affiliation: Department of Environmental Conservation, University of Massachusetts, Amherst, Massachusetts, United States of America

    X
  • Michael K. Schwartz

    Affiliation: United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America

    X
  • Published: March 26, 2013
  • DOI: 10.1371/journal.pone.0059520

About the Authors

Taylor M. Wilcox, Kevin S. McKelvey, Michael K. Young, Michael K. Schwartz
United States Department of Agriculture Forest Service, Rocky Mountain Research Station, Missoula, Montana, United States of America
Stephen F. Jane, Andrew R. Whiteley
Department of Environmental Conservation, University of Massachusetts, Amherst, Massachusetts, United States of America
Winsor H. Lowe
Division of Biological Sciences, University of Montana, Missoula, Montana, United States of America

Corresponding Author

Email: taylor.wilcox@umontana.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TMW KSM MKY SFJ WHL ARW MKS. Performed the experiments: TMW SFJ. Analyzed the data: TMW. Contributed reagents/materials/analysis tools: KSM MKY SFJ MKS. Wrote the paper: TMW KSM MKY SFJ WHL ARW MKS.