About the Authors

William L. Rice

Affiliation Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

Alfred N. Van Hoek

Affiliation Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

Teodor G. Păunescu

Affiliation Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

Chuong Huynh

Affiliation Carl Zeiss Microscopy, Peabody, Massachusetts, United States of America

Bernhard Goetze

Affiliation Carl Zeiss Microscopy, Peabody, Massachusetts, United States of America

Bipin Singh

Affiliation Carl Zeiss Microscopy, Peabody, Massachusetts, United States of America

Larry Scipioni

Affiliation Carl Zeiss Microscopy, Peabody, Massachusetts, United States of America

Lewis A. Stern

Affiliation Carl Zeiss Microscopy, Peabody, Massachusetts, United States of America

Dennis Brown

brown.dennis@mgh.harvard.edu

Affiliation Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

Competing Interests

The authors have read the journal's policy and have the following conflicts. The work was performed partially with a sponsored research agreement between the Massachusetts General Hospital and the Zeiss Corporation. Four of the authors are employed by the Zeiss Corporation in the Helium Ion Microscopy Facility in Peabody, MA. The corresponding author D. Brown is the PI of the sponsored research agreement from the Zeiss Corporation. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: WLR AVH TGP BG DB. Performed the experiments: WLR AVH TGP CH LS BG DB. Analyzed the data: WLR AVH TGP DG CH LS BG DB. Contributed reagents/materials/analysis tools: WLR AVH TGP CH BG BS LS LAS DB. Wrote the paper: WLR AVH TGP DB.