About the Authors

Meral Tunc-Ozdemir

Contributed equally to this work with: Meral Tunc-Ozdemir, Claudia Rato

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Claudia Rato

Contributed equally to this work with: Meral Tunc-Ozdemir, Claudia Rato

Current address: European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom

Affiliation Universidade de Lisboa, Faculdade de Ciências de Lisboa, BioFIG, Lisboa, Portugal

Elizabeth Brown

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Stephanie Rogers

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Amanda Mooneyham

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Sabine Frietsch

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Candace T. Myers

Current address: Department of Cellular and Molecular Medicine, Molecular Cardiovascular Research Program, University of Arizona, Tucson, Arizona, United States of America

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Lisbeth Rosager Poulsen

Affiliations Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America, Department of Plant Biology and Biotechnology, Centre for Membrane Pumps in Cells and Disease (PUMPKIN), University of Copenhagen, Danish National Research Foundation, Frederiksberg, Denmark

Rui Malhó

Affiliation Universidade de Lisboa, Faculdade de Ciências de Lisboa, BioFIG, Lisboa, Portugal

Jeffrey F. Harper

jfharper@unr.edu

Affiliation Department of Biochemistry, University of Nevada, Reno, Nevada, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MTO CR EB SR AM SF CTM LRP RM JFH. Performed the experiments: MTO CR EB SR AM SF CTM LRP. Wrote the manuscript and provided significant experimental results: MTO. Helped writing, design experiments and provided significant experimental results, namely the discovery of pollen transmision defect: CR. Provided significant experimental results, specifically in genotyping and finding rescue lines: EB. Provided significant experimental results, specifically with confocal microscopy: SR. Provided significant experimental results, specifically in generating F to W mutations: AM. Helped design experiments, and provided significant experimental results, specifically in comparisons with cngc18: SF. Provided significant experimental results, specifically in genotyping and finding rescue lines: CTM. Identified the 2 of the original cngc alleles used in this study: LRP. Helped write and design experiments: RM. Helped write, design experiments, analyzed the bursting phenotype: JFH. Analyzed the data: MTO CR EB SR AM SF CTM LRP RM JFH. Contributed reagents/materials/analysis tools: MTO CR EB SR AM SF CTM LRP RM JFH. Wrote the paper: MTO CR RM JFH.