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Research Article

A Yeast Purification System for Human Translation Initiation Factors eIF2 and eIF2Bε and Their Use in the Diagnosis of CACH/VWM Disease

  • Rogerio A. de Almeida,

    Affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom

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  • Anne Fogli,

    Affiliations: Laboratoire GReD (Génétique, Reproduction et Développement), Faculté de Médecine, INSERM U1103 CNRS 6293, Clermont-Ferrand, France, Université de Clermont, UFR Médecine, Clermont-Ferrand, France, Centre Hospitalier Universitaire de Clermont-Ferrand, Service de Biochimie Médicale et Biologie Moléculaire, Clermont-Ferrand, France

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  • Marina Gaillard,

    Affiliation: Laboratoire GReD (Génétique, Reproduction et Développement), Faculté de Médecine, INSERM U1103 CNRS 6293, Clermont-Ferrand, France

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  • Gert C. Scheper,

    Affiliation: Department of Pediatrics, VU University Medical Centre, Amsterdam, The Netherlands

    Current address: Crucell Holland BV, Leiden, The Netherlands

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  • Odile Boesflug-Tanguy,

    Affiliations: INSERM U676, Hopital Robert Debré, Paris, France, Université Paris Diderot, Sorbonne Cité, UMR U676, Paris, France, APHP, Hopital Robert Debré, Pediatric Neurology and Metabolic Diseases, Reference Center for Leukodystrophies, Paris, France

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  • Graham D. Pavitt mail

    graham.pavitt@manchester.ac.uk

    Affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom

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  • Published: January 15, 2013
  • DOI: 10.1371/journal.pone.0053958

About the Authors

Rogerio A. de Almeida, Graham D. Pavitt
Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
Anne Fogli, Marina Gaillard
Laboratoire GReD (Génétique, Reproduction et Développement), Faculté de Médecine, INSERM U1103 CNRS 6293, Clermont-Ferrand, France
Anne Fogli
Université de Clermont, UFR Médecine, Clermont-Ferrand, France
Anne Fogli
Centre Hospitalier Universitaire de Clermont-Ferrand, Service de Biochimie Médicale et Biologie Moléculaire, Clermont-Ferrand, France
Gert C. Scheper
Department of Pediatrics, VU University Medical Centre, Amsterdam, The Netherlands
Odile Boesflug-Tanguy
INSERM U676, Hopital Robert Debré, Paris, France
Odile Boesflug-Tanguy
Université Paris Diderot, Sorbonne Cité, UMR U676, Paris, France
Odile Boesflug-Tanguy
APHP, Hopital Robert Debré, Pediatric Neurology and Metabolic Diseases, Reference Center for Leukodystrophies, Paris, France

Corresponding Author

Email: graham.pavitt@manchester.ac.uk

Competing Interests

Dr. Scheper is an employee Crucell Holland BV. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: GDP GCS OBT. Performed the experiments: RAA AF MG. Analyzed the data: RAA AF GDP. Contributed reagents/materials/analysis tools: GCS OBT GDP. Wrote the paper: GDP AF OBT RAA GCS.