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Research Article

An Automated HIV-1 Env-Pseudotyped Virus Production for Global HIV Vaccine Trials

  • Anke Schultz equal contributor,

    equal contributor Contributed equally to this work with: Anke Schultz, Stefanie Koch

    Affiliation: Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany

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  • Stefanie Koch equal contributor,

    equal contributor Contributed equally to this work with: Anke Schultz, Stefanie Koch

    Affiliation: Department of Virology, Saarland University, Homburg, Germany

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  • Martina Fuss,

    Affiliation: Department of Virology, Saarland University, Homburg, Germany

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  • Angela S. Mazzotta,

    Affiliation: Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany

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  • Marcella Sarzotti-Kelsoe,

    Affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

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  • Daniel A. Ozaki,

    Affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

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  • David C. Montefiori,

    Affiliation: Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

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  • Hagen von Briesen,

    Affiliation: Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany

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  • Heiko Zimmermann,

    Affiliation: Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany

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  • Andreas Meyerhans mail

    andreas.meyerhans@upf.edu

    Affiliations: Department of Virology, Saarland University, Homburg, Germany, ICREA Infection Biology Laboratory, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain

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  • Published: December 27, 2012
  • DOI: 10.1371/journal.pone.0051715

About the Authors

Anke Schultz, Angela S. Mazzotta, Hagen von Briesen, Heiko Zimmermann
Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany
Stefanie Koch, Martina Fuss, Andreas Meyerhans
Department of Virology, Saarland University, Homburg, Germany
Marcella Sarzotti-Kelsoe, Daniel A. Ozaki, David C. Montefiori
Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
Andreas Meyerhans
ICREA Infection Biology Laboratory, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain

Corresponding Author

Email: andreas.meyerhans@upf.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SK AS MS-K DAO. Performed the experiments: SK AS MF ASM. Analyzed the data: SK AS MS-K DAO. Contributed reagents/materials/analysis tools: DM. Wrote the paper: AM AS. Conceptional outline and participating by adding key points to the manuscript: AM MS-K DM HVB HZ.