About the Authors
- Ann L. McEvoy
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* E-mail: almcevoy@berkeley.edu (ALM); robert.e.campbell@ualberta.ca (REC)
¶a Correspondence related to super-resolution imaging techniques should be addressed to ALM.
Affiliation Biophysics Graduate Group, University of California, Berkeley, California, United States of America
- Hiofan Hoi
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Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
- Mark Bates
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Affiliation Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
- Evgenia Platonova
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Affiliation Institute of Biochemistry, Eidgenössische Technische Hochschule (ETH) Zurich, Zurich, Switzerland
- Paula J. Cranfill
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Affiliations National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America, Department of Physics, University of California, Berkeley, California, United States of America
- Michelle A. Baird
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Affiliations National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America, Department of Physics, University of California, Berkeley, California, United States of America
- Michael W. Davidson
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Affiliations National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America, Department of Physics, University of California, Berkeley, California, United States of America
- Helge Ewers
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Affiliation Institute of Biochemistry, Eidgenössische Technische Hochschule (ETH) Zurich, Zurich, Switzerland
- Jan Liphardt
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Affiliations Biophysics Graduate Group, University of California, Berkeley, California, United States of America, California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California, United States of America, Bay Area Physical Sciences – Oncology Center, University of California, Berkeley, California, United States of America, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America
- Robert E. Campbell
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* E-mail: almcevoy@berkeley.edu (ALM); robert.e.campbell@ualberta.ca (REC)
¶b Correspondence related to new pcFP variants used in this work should be addressed to REC.
Affiliation Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
Competing Interests
New pcFPs that originate from the Campbell lab and are described in this manuscript are covered by US patent application 12/960,397, which is titled “Photoconvertible fluorescent proteins” and is jointly owned by the University of Alberta and Allele Biotechnology. Allele Biotechnology is also the licensed distributor of plasmids containing genes encoding these pcFPs. There are no further patents, products in development or marketed products to declare. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.
Author Contributions
Conceived and designed the experiments: ALM HH MB EP HE JTL REC. Performed the experiments: ALM HH MB EP PJC MAB MD. Analyzed the data: ALM HH MB EP HE PJC MAB MD REC. Contributed reagents/materials/analysis tools: MB HE MD JTL REC. Wrote the manuscript: ALM REC HH JTL MB EP HE MD. Designed the protein engineering assay and conceived of the in vitro experiments: HH REC. Conceived of the imaging experiments: ALM MB JTL. Conceived of the experiments using mammalian cells: MWD EP HE. Engineered mMaple and performed the in vitro characterization of the protein: HH REC. Made the bacterial strains used for imaging and performed the bacterial cell imaging: ALM. Constructed the (f−)PALM/STORM microscope used: MB. Performed the imaging of purified mMaple: ALM MB. Created the pcFP-actin fusions used for mammalian cell imaging, and performed flow cytometry experiments: HH. In depth comparison of pcFP-actin fusions: EP HE. Determined the photobleaching and photoconversion rates of pcFP-H2B fusions: PJC MAB MWD. Analyzed data relating to in vitro characterization of mMaple: HH REC. Developed custom software to analyze (f)PALM/STORM data: ALM MB. Analyzed 3D-SIM data: ALM. Analyzed mammalian cell data: EP HE PJC MAB MWD. Developed the software for (f−)PALM/STORM image reconstruction: MB.