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Research Article

Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

  • Jolien Vermeire,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Evelien Naessens,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Hanne Vanderstraeten,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Alessia Landi,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Veronica Iannucci,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Anouk Van Nuffel,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Tom Taghon,

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Massimo Pizzato,

    Affiliation: Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy

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  • Bruno Verhasselt mail

    bruno.verhasselt@ugent.be

    Affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium

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  • Published: December 05, 2012
  • DOI: 10.1371/journal.pone.0050859

About the Authors

Jolien Vermeire, Evelien Naessens, Hanne Vanderstraeten, Alessia Landi, Veronica Iannucci, Anouk Van Nuffel, Tom Taghon, Bruno Verhasselt
Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University, Ghent, Belgium
Massimo Pizzato
Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy

Corresponding Author

Email: bruno.verhasselt@ugent.be

Competing Interests

Bruno Verhasselt serves as an academic editor of PLOS ONE. No other conflicts of interest are to be reported by the authors. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: JV MP BV. Performed the experiments: JV EN HV. Analyzed the data: JV BV. Contributed reagents/materials/analysis tools: AL VI AVN TT. Wrote the paper: JV BV.