One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

Fatma Filiz Coskun-Ari; Dilek Guldemir; Riza Durmaz

doi:10.1371/journal.pone.0050406

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Open Access

Peer-reviewed

Research Article

One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

Fatma Filiz Coskun-Ari,

Affiliation Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey
⨯
Dilek Guldemir,

Affiliation Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey
⨯
Riza Durmaz

* E-mail: rizadurmaz@ymail.com

Affiliations Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey, Department of Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey
⨯

One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

Fatma Filiz Coskun-Ari,
Dilek Guldemir,
Riza Durmaz

Published: December 4, 2012
https://doi.org/10.1371/journal.pone.0050406

About the Authors

Fatma Filiz Coskun-Ari

Affiliation Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey

Dilek Guldemir

Affiliation Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey

Riza Durmaz

* E-mail: rizadurmaz@ymail.com

Affiliations Molecular Microbiology Research and Application Laboratory, Department of Microbiology Reference Laboratories, Public Health Agency of Turkey, Ankara, Turkey, Department of Clinical Microbiology, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey

Competing Interests

A patent application has been filed for the one-step multiplex PCR assay (TR2011/08227).

Author Contributions

Conceived and designed the experiments: FFCA RD. Performed the experiments: FFCA DG. Analyzed the data: FFCA DG RD. Contributed reagents/materials/analysis tools: FFCA DG. Wrote the paper: FFA. Critically revised the manuscript: RD.

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Polymerase chain reaction
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Pneumococcus
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Immune serum
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Infectious disease epidemiology
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Quellung reaction
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Gel electrophoresis
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Magnesium chloride
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Polysaccharides
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