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Research Article

Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions

  • Yangbo Hu equal contributor,

    equal contributor Contributed equally to this work with: Yangbo Hu, Lipeng Feng

    Affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

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  • Lipeng Feng equal contributor,

    equal contributor Contributed equally to this work with: Yangbo Hu, Lipeng Feng

    Affiliations: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China, Graduate University of the Chinese Academy of Sciences, Beijing, China

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  • Yunlong Li,

    Affiliations: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China, Graduate University of the Chinese Academy of Sciences, Beijing, China

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  • Yong Zhang,

    Affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

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  • Pei Lu,

    Affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

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  • Simon Rayner,

    Affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

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  • Shiyun Chen mail

    sychen@wh.iov.cn

    Affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

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  • Published: November 21, 2012
  • DOI: 10.1371/journal.pone.0050142

About the Authors

Yangbo Hu, Lipeng Feng, Yunlong Li, Yong Zhang, Pei Lu, Simon Rayner, Shiyun Chen
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
Lipeng Feng, Yunlong Li
Graduate University of the Chinese Academy of Sciences, Beijing, China

Corresponding Author

Email: sychen@wh.iov.cn

Competing Interests

Although the authors have filed a patent application (#CN20120076722.6) in China relating to the method developed in this article, this does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. The authors declare there are no other relevant declarations relating to employment, consultancy, patents, products in development or marketed products, etc.

Author Contributions

Conceived and designed the experiments: YH LF SC. Performed the experiments: YH LF YL. Analyzed the data: YH LF SC. Contributed reagents/materials/analysis tools: YZ PL. Wrote the paper: YH SR SC.