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Research Article

Direct and Dynamic Detection of HIV-1 in Living Cells

  • Jonas Helma,

    Affiliations: Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany, Center for Integrated Protein Science, Munich, Germany

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  • Katrin Schmidthals,

    Affiliations: Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany, ChromoTek GmbH, Planegg-Martinsried, Germany

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  • Vanda Lux,

    Affiliation: Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany

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  • Stefan Nüske,

    Affiliation: Livestock Center of the Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Oberschleissheim, Germany

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  • Armin M. Scholz,

    Affiliation: Livestock Center of the Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Oberschleissheim, Germany

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  • Hans-Georg Kräusslich,

    Affiliation: Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany

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  • Ulrich Rothbauer mail,

    ulrich.rothbauer@uni-tuebingen.de (UR); h.leonhardt@lmu.de (HL)

    Affiliations: Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany, ChromoTek GmbH, Planegg-Martinsried, Germany

    Current address: Pharmaceutical Biotechnology, Eberhard-Karls University Tuebingen, Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany

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  • Heinrich Leonhardt mail

    ulrich.rothbauer@uni-tuebingen.de (UR); h.leonhardt@lmu.de (HL)

    Affiliations: Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany, Center for Integrated Protein Science, Munich, Germany

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  • Published: November 28, 2012
  • DOI: 10.1371/journal.pone.0050026

About the Authors

Jonas Helma, Katrin Schmidthals, Ulrich Rothbauer, Heinrich Leonhardt
Department of Biology II, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany
Vanda Lux, Hans-Georg Kräusslich
Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany
Stefan Nüske, Armin M. Scholz
Livestock Center of the Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Oberschleissheim, Germany
Jonas Helma, Heinrich Leonhardt
Center for Integrated Protein Science, Munich, Germany
Katrin Schmidthals, Ulrich Rothbauer
ChromoTek GmbH, Planegg-Martinsried, Germany

Corresponding Authors

Competing Interests

JH, KS, UR and HL are shareholders of the commercial company ChromoTek GmbH. However, this does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. No patents, products in development or marketed products relating to the subject of this study are available or planned. The reagents described in this study will be available upon request.

Author Contributions

Conceived and designed the experiments: JH HGK UR HL. Performed the experiments: JH KS. Analyzed the data: JH KS HGK UR HL. Contributed reagents/materials/analysis tools: VL SN AMS. Wrote the paper: JH HGK UR HL.