Research Article

High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

  • Audrey D. McConnell,
  • Minjee Do,
  • Tamlyn Y. Neben,
  • Vladimir Spasojevic,
  • Josh MacLaren,
  • Andy P. Chen,
  • Laurence Altobell III,
  • John L. Macomber,
  • Ashley D. Berkebile,
  • Robert A. Horlick,
  • Peter M. Bowers mail,

    Affiliation: AnaptysBio, Inc., San Diego, California, United States of America

  • David J. King
  • Published: November 14, 2012
  • DOI: 10.1371/journal.pone.0049458

About the Authors

Peter M. Bowers
AnaptysBio, Inc., San Diego, California, United States of America

Corresponding Author


Competing Interests

The authors have the following interests. AnaptysBio, Inc. funded the research described in this report. AnaptysBio encourages publication and plays a role in issuing approval for the authors to submit scientific manuscripts to journals. All authors are employed by AnaptysBio, Inc. and the technology described in this paper is a product of AnaptysBio ( There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: ADM TYN RAH PMB DJK. Performed the experiments: ADM MD TYN VS JM APC LA JLM ADB. Analyzed the data: ADM MD TYN VS JM APC JLM ADB. Wrote the paper: ADM.