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Research Article

Transcutaneous Application of Carbon Dioxide (CO2) Induces Mitochondrial Apoptosis in Human Malignant Fibrous Histiocytoma In Vivo

  • Yasuo Onishi,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Teruya Kawamoto mail,

    trykwmt@med.kobe-u.ac.jp

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Takeshi Ueha,

    Affiliation: NeoChemir Inc., Gokodori, Chuo-ku, Kobe, Hyogo, Japan

    X
  • Kenta Kishimoto,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Hitomi Hara,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Naomasa Fukase,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Mitsunori Toda,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Risa Harada,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Masaya Minoda,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Yoshitada Sakai,

    Affiliation: Faculty of Health Care Sciences, Himeji Dokkyo University, Kami-Ohno, Himeji, Hyogo, Japan

    X
  • Masahiko Miwa,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

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  • Masahiro Kurosaka,

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

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  • Toshihiro Akisue

    Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan

    X
  • Published: November 15, 2012
  • DOI: 10.1371/journal.pone.0049189

About the Authors

Yasuo Onishi, Teruya Kawamoto, Kenta Kishimoto, Hitomi Hara, Naomasa Fukase, Mitsunori Toda, Risa Harada, Masaya Minoda, Masahiko Miwa, Masahiro Kurosaka, Toshihiro Akisue
Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kusunoki-cho, Chuo-ku, Kobe, Japan
Takeshi Ueha
NeoChemir Inc., Gokodori, Chuo-ku, Kobe, Hyogo, Japan
Yoshitada Sakai
Faculty of Health Care Sciences, Himeji Dokkyo University, Kami-Ohno, Himeji, Hyogo, Japan

Corresponding Author

Email: trykwmt@med.kobe-u.ac.jp

Competing Interests

This study was supported by NeoChemir Inc. through the employment of Takeshi Ueha who conceived, designed and performed the experiments, and analyzed the data for this study. The hydro-gel used in this study was a gift from NeoChemir Inc. and is patented by Neochemir Inc. (publication number WO2004/002393; publication date January 8, 2004). There are no other patents, products in development or other marketed products to declare. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: YO TK TU. Performed the experiments: YO TK TU M. Minoda. Analyzed the data: YO MT RH HH NF. Contributed reagents/materials/analysis tools: TU. Wrote the paper: YO TK TU. Supervised all aspects of this study: KK M. Miwa YS MK TA.