Advertisement
Research Article

HIV-1 Tat Promotes Integrin-Mediated HIV Transmission to Dendritic Cells by Binding Env Spikes and Competes Neutralization by Anti-HIV Antibodies

  • Paolo Monini,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Aurelio Cafaro,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Indresh K. Srivastava,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America

    X
  • Sonia Moretti,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Victoria A. Sharma,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America

    X
  • Claudia Andreini,

    Affiliation: CERM, University of Florence, Florence, Italy

    X
  • Chiara Chiozzini,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Flavia Ferrantelli,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Maria R. Pavone. Cossut,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Antonella Tripiciano,

    Affiliations: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy, San Gallicano Hospital, Rome, Italy

    X
  • Filomena Nappi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy

    X
  • Olimpia Longo,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Stefania Bellino,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Orietta Picconi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Emanuele Fanales-Belasio,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Alessandra Borsetti,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Elena Toschi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy

    X
  • Ilaria Schiavoni,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy

    X
  • Ilaria Bacigalupo,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Elaine Kan,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America

    X
  • Leonardo Sernicola,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Maria T. Maggiorella,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Katy Montin,

    Affiliation: Department of Biochemistry, University of Ferrara, Ferrara, Italy

    X
  • Marco Porcu,

    Affiliation: CERM, University of Florence, Florence, Italy

    X
  • Patrizia Leone,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Pasqualina Leone,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy

    X
  • Barbara Collacchi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Clelia Palladino,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Barbara Ridolfi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy

    X
  • Mario Falchi,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Iole Macchia,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    Current address: Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy

    X
  • Jeffrey B. Ulmer,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America

    X
  • Stefano Buttò,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Cecilia Sgadari,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Mauro Magnani,

    Affiliation: Department of Biomolecular Science, University of Urbino, Urbino, Italy

    X
  • Maurizio P. M. Federico,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Fausto Titti,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Lucia Banci,

    Affiliation: CERM, University of Florence, Florence, Italy

    X
  • Franco Dallocchio,

    Affiliation: Department of Biochemistry, University of Ferrara, Ferrara, Italy

    X
  • Rino Rappuoli,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Siena, Italy

    X
  • Fabrizio Ensoli,

    Affiliation: San Gallicano Hospital, Rome, Italy

    X
  • Susan W. Barnett,

    Affiliation: Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America

    X
  • Enrico Garaci,

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Barbara Ensoli mail

    barbara.ensoli@iss.it

    Affiliation: National AIDS Center, Istituto Superiore di Sanità, Rome, Italy

    X
  • Published: November 13, 2012
  • DOI: 10.1371/journal.pone.0048781

About the Authors

Paolo Monini, Aurelio Cafaro, Sonia Moretti, Chiara Chiozzini, Flavia Ferrantelli, Maria R. Pavone. Cossut, Antonella Tripiciano, Filomena Nappi, Olimpia Longo, Stefania Bellino, Orietta Picconi, Emanuele Fanales-Belasio, Alessandra Borsetti, Elena Toschi, Ilaria Schiavoni, Ilaria Bacigalupo, Leonardo Sernicola, Maria T. Maggiorella, Patrizia Leone, Pasqualina Leone, Barbara Collacchi, Clelia Palladino, Barbara Ridolfi, Mario Falchi, Iole Macchia, Stefano Buttò, Cecilia Sgadari, Maurizio P. M. Federico, Fausto Titti, Enrico Garaci, Barbara Ensoli
National AIDS Center, Istituto Superiore di Sanità, Rome, Italy
Indresh K. Srivastava, Victoria A. Sharma, Elaine Kan, Jeffrey B. Ulmer, Susan W. Barnett
Novartis Vaccines & Diagnostics, Inc., Cambridge, Massachusetts, United States of America
Claudia Andreini, Marco Porcu, Lucia Banci
CERM, University of Florence, Florence, Italy
Katy Montin, Franco Dallocchio
Department of Biochemistry, University of Ferrara, Ferrara, Italy
Antonella Tripiciano, Fabrizio Ensoli
San Gallicano Hospital, Rome, Italy
Rino Rappuoli
Novartis Vaccines & Diagnostics, Inc., Siena, Italy
Mauro Magnani
Department of Biomolecular Science, University of Urbino, Urbino, Italy

Corresponding Author

Email: barbara.ensoli@iss.it

Competing Interests

IKS, VAS, EK, JBU, RR and SWB are employees of Novartis Vaccines & Diagnostics, Inc. Novartis provided the recombinant HIV-1 Env proteins as a partner in several collaborative studies (the European projects AVIP and MUVAPRED, the “Joint Program ISS/Novartis (Chiron) for the development of a combined vaccine against HIV/AIDS.” There is one patent to declare: Novel Tat complexes, and vaccines comprising them (Inventor: Ensoli B.; Applicant: ISS (100%); priority date March 11, 2004; N. PCT/EP2005/003043, Publication N. WO2005/090391). There are no products in development or marketed products to declare. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: BE PM. Performed the experiments: VAS EK IKS SM MRPC IM ET IB CP Patrizia Leone EFB MPMF CA MP LB FT LS MTM MF FF AB IS JBU BR KM FD CC FN Pasqualina Leone BC CS. Analyzed the data: AC S. Bellino MM FE AT OL S. Buttò OP. Wrote the paper: BE PM AC. Coordinated the studies performed in Novartis: RR JBU SWB. Contributed to data analysis and discussion: EG.