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Research Article

Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent

  • Fumiaki Itoi,

    Affiliations: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan, The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan

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  • Mikiko Tokoro,

    Affiliations: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan, The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan

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  • Yukari Terashita,

    Affiliations: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan, Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan

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  • Kazuo Yamagata,

    Affiliation: Center for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, Suita, Japan

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  • Noritaka Fukunaga,

    Affiliation: The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan

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  • Yoshimasa Asada,

    Affiliation: The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan

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  • Teruhiko Wakayama mail

    teru@cdb.riken.jp

    Affiliations: Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan, Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan, Kofu, Japan

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  • Published: October 09, 2012
  • DOI: 10.1371/journal.pone.0047512

About the Authors

Fumiaki Itoi, Mikiko Tokoro, Yukari Terashita, Teruhiko Wakayama
Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan
Fumiaki Itoi, Mikiko Tokoro, Noritaka Fukunaga, Yoshimasa Asada
The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan
Yukari Terashita
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Kazuo Yamagata
Center for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
Teruhiko Wakayama
Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan, Kofu, Japan

Corresponding Author

Email: teru@cdb.riken.jp

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: TW FI KY YA. Performed the experiments: FI MT YT. Analyzed the data: FI NF. Contributed reagents/materials/analysis tools: TW. Wrote the paper: TW FI.