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Research Article

Experimental and In Silico Modelling Analyses of the Gene Expression Pathway for Recombinant Antibody and By-Product Production in NS0 Cell Lines

  • Emma J. Mead mail,

    E.J.Mead@kent.ac.uk (EJM); C.M.Smales@kent.ac.uk (CMS); T.von-der-Haar@kent.ac.uk (TvdH)

    Affiliations: School of Biosciences, University of Kent, Canterbury, United Kingdom, Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom

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  • Lesley M. Chiverton,

    Affiliations: School of Biosciences, University of Kent, Canterbury, United Kingdom, Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom

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  • Sarah K. Spurgeon,

    Affiliations: School of Engineering and Digital Arts, University of Kent, Canterbury, United Kingdom, Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom

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  • Elaine B. Martin,

    Affiliation: School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle, United Kingdom

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  • Gary A. Montague,

    Affiliation: School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle, United Kingdom

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  • C. Mark Smales mail,

    E.J.Mead@kent.ac.uk (EJM); C.M.Smales@kent.ac.uk (CMS); T.von-der-Haar@kent.ac.uk (TvdH)

    Affiliations: School of Biosciences, University of Kent, Canterbury, United Kingdom, Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom

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  • Tobias von der Haar mail

    E.J.Mead@kent.ac.uk (EJM); C.M.Smales@kent.ac.uk (CMS); T.von-der-Haar@kent.ac.uk (TvdH)

    Affiliations: School of Biosciences, University of Kent, Canterbury, United Kingdom, Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom

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  • Published: October 10, 2012
  • DOI: 10.1371/journal.pone.0047422

About the Authors

Emma J. Mead, Lesley M. Chiverton, C. Mark Smales, Tobias von der Haar
School of Biosciences, University of Kent, Canterbury, United Kingdom
Sarah K. Spurgeon
School of Engineering and Digital Arts, University of Kent, Canterbury, United Kingdom
Emma J. Mead, Lesley M. Chiverton, Sarah K. Spurgeon, C. Mark Smales, Tobias von der Haar
Centre for Molecular Processing, University of Kent, Canterbury, United Kingdom
Elaine B. Martin, Gary A. Montague
School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle, United Kingdom

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: CMS TVDH. Performed the experiments: EJM LMC. Analyzed the data: SKS EBM GAM CMS TVDH. Wrote the paper: TVDH CMS EJM SKS. Implemented and analysed the models: TVDH SKS. Analysed modelling results: EJM LMC SKS EBM GAM CMS TVDH. Read and approved the manuscript: EJM LMC SKS EBM GAM CMS TVDH.