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Research Article

Neurally Mediated Airway Constriction in Human and Other Species: A Comparative Study Using Precision-Cut Lung Slices (PCLS)

  • Marco Schlepütz,

    Affiliation: Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany

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  • Annette D. Rieg,

    Affiliation: Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany

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  • Sophie Seehase,

    Affiliations: Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany, Encepharm GmbH, Göttingen, Germany

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  • Jan Spillner,

    Affiliation: Department of Cardiothoracic and Vascular Surgery, RWTH Aachen University, Aachen, Germany

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  • Alberto Perez-Bouza,

    Affiliation: Institute of Pathology, RWTH Aachen University, Aachen, Germany

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  • Till Braunschweig,

    Affiliation: Institute of Pathology, RWTH Aachen University, Aachen, Germany

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  • Thomas Schroeder,

    Affiliation: Department of Surgery, Luisenhospital Aachen, Aachen, Germany

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  • Marc Bernau,

    Affiliation: Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany

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  • Verena Lambermont,

    Affiliation: School of Oncology and Developmental Biology, School of Mental Health and Neuroscience, Maastricht University Medical Center, Maastricht, The Netherlands

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  • Christina Schlumbohm,

    Affiliation: Encepharm GmbH, Göttingen, Germany

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  • Katherina Sewald,

    Affiliation: Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany

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  • Rüdiger Autschbach,

    Affiliation: Department of Cardiothoracic and Vascular Surgery, RWTH Aachen University, Aachen, Germany

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  • Armin Braun,

    Affiliation: Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany

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  • Boris W. Kramer,

    Affiliation: School of Oncology and Developmental Biology, School of Mental Health and Neuroscience, Maastricht University Medical Center, Maastricht, The Netherlands

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  • Stefan Uhlig,

    Affiliation: Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany

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  • Christian Martin mail

    chmartin@ukaachen.de

    Affiliation: Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany

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  • Published: October 09, 2012
  • DOI: 10.1371/journal.pone.0047344

About the Authors

Marco Schlepütz, Annette D. Rieg, Marc Bernau, Stefan Uhlig, Christian Martin
Institute of Pharmacology and Toxicology, RWTH Aachen University, Aachen, Germany
Sophie Seehase, Katherina Sewald, Armin Braun
Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany
Sophie Seehase, Christina Schlumbohm
Encepharm GmbH, Göttingen, Germany
Jan Spillner, Rüdiger Autschbach
Department of Cardiothoracic and Vascular Surgery, RWTH Aachen University, Aachen, Germany
Alberto Perez-Bouza, Till Braunschweig
Institute of Pathology, RWTH Aachen University, Aachen, Germany
Thomas Schroeder
Department of Surgery, Luisenhospital Aachen, Aachen, Germany
Verena Lambermont, Boris W. Kramer
School of Oncology and Developmental Biology, School of Mental Health and Neuroscience, Maastricht University Medical Center, Maastricht, The Netherlands

Corresponding Author

Email: chmartin@ukaachen.de

Competing Interests

CS is currently employee at Encepharm GmbH. With respect to the current study CS took responsibility for the supply with NHP material from the German Primate Center Göttingen, a non-commercial institution. There are no competing interests by Encepharm GmbH relating to employment, consultancy, patents, products in development or marketed products. Hence, CS's employment at Encepharm GmbH does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MS SU CM. Performed the experiments: MS MB. Analyzed the data: MS SU CM. Contributed reagents/materials/analysis tools: MS ADR SS JS AP TB TS VL CS KS RA AB BWK SU CM. Wrote the paper: MS SU CM. Read and revised the manuscript: MS ADR SS JS AP TB TS VL CS KS RA AB BWK SU CM.