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Research Article

Impact of Cell Type and Epitope Tagging on Heterologous Expression of G Protein-Coupled Receptor: A Systematic Study on Angiotensin Type II Receptor

  • Lili Jiang,

    Affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

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  • Gladys M. K. Teng,

    Affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

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  • Elaine Y. M. Chan,

    Affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

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  • Shannon W. N. Au,

    Affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

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  • Helen Wise,

    Affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

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  • Susanna S. T. Lee mail,

    wtcheung@cuhk.edu.hk (WTC); lee2022@cuhk.edu.hk (SL)

    Affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    X
  • Wing-Tai Cheung mail

    wtcheung@cuhk.edu.hk (WTC); lee2022@cuhk.edu.hk (SL)

    Affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    X
  • Published: October 08, 2012
  • DOI: 10.1371/journal.pone.0047016

About the Authors

Lili Jiang, Gladys M. K. Teng, Elaine Y. M. Chan, Helen Wise, Wing-Tai Cheung
School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Shannon W. N. Au, Susanna S. T. Lee
School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: LJ GT WTC. Performed the experiments: LJ GT EC. Analyzed the data: LJ GT HW SL WTC. Contributed reagents/materials/analysis tools: SA SL WTC. Wrote the paper: LJ WTC.