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Research Article

Targeted Genomic Integration of a Selectable Floxed Dual Fluorescence Reporter in Human Embryonic Stem Cells

  • Jay A. Gantz equal contributor,

    equal contributor Contributed equally to this work with: Jay A. Gantz, Nathan J. Palpant

    Affiliation: Department of Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

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  • Nathan J. Palpant equal contributor,

    equal contributor Contributed equally to this work with: Jay A. Gantz, Nathan J. Palpant

    Affiliation: Department of Pathology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

    X
  • Robert E. Welikson,

    Affiliation: Department of Biochemistry, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

    X
  • Stephen D. Hauschka,

    Affiliation: Department of Biochemistry, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

    X
  • Charles E. Murry,

    Affiliations: Department of Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America, Department of Pathology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America, Department of Medicine/Cardiology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

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  • Michael A. Laflamme mail

    laflamme@u.washington.edu

    Affiliation: Department of Pathology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

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  • Published: October 10, 2012
  • DOI: 10.1371/journal.pone.0046971

About the Authors

Jay A. Gantz, Charles E. Murry
Department of Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America
Nathan J. Palpant, Charles E. Murry, Michael A. Laflamme
Department of Pathology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America
Robert E. Welikson, Stephen D. Hauschka
Department of Biochemistry, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America
Charles E. Murry
Department of Medicine/Cardiology, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America

Corresponding Author

Email: laflamme@u.washington.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JAG NJP CEM MAL. Performed the experiments: JAG NJP. Analyzed the data: JAG NJP. Contributed reagents/materials/analysis tools: JAG NJP REW SDH. Wrote the paper: JAG NJP.