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Research Article

Role of N-Terminal His6-Tags in Binding and Efficient Translocation of Polypeptides into Cells Using Anthrax Protective Antigen (PA)

  • Christoph Beitzinger equal contributor,

    equal contributor Contributed equally to this work with: Christoph Beitzinger, Caroline Stefani

    Affiliation: Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany

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  • Caroline Stefani equal contributor,

    equal contributor Contributed equally to this work with: Christoph Beitzinger, Caroline Stefani

    Affiliations: Toxines microbiennes dans la relation hôte-pathogènes, C3M, U1065, Inserm, Nice, France, UFR Médecine, IFR50, Université de Nice-Sophia Antipolis, Nice, France

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  • Angelika Kronhardt,

    Affiliation: Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany

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  • Monica Rolando,

    Affiliations: Toxines microbiennes dans la relation hôte-pathogènes, C3M, U1065, Inserm, Nice, France, UFR Médecine, IFR50, Université de Nice-Sophia Antipolis, Nice, France

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  • Gilles Flatau,

    Affiliation: Toxines microbiennes dans la relation hôte-pathogènes, C3M, U1065, Inserm, Nice, France

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  • Emmanuel Lemichez mail,

    Emmanuel.Lemichez@unice.fr (EL); roland.benz@uni-wuerzburg.de (RB)

    Affiliations: Toxines microbiennes dans la relation hôte-pathogènes, C3M, U1065, Inserm, Nice, France, UFR Médecine, IFR50, Université de Nice-Sophia Antipolis, Nice, France

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  • Roland Benz mail

    Emmanuel.Lemichez@unice.fr (EL); roland.benz@uni-wuerzburg.de (RB)

    Affiliations: Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany

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  • Published: October 08, 2012
  • DOI: 10.1371/journal.pone.0046964

About the Authors

Christoph Beitzinger, Angelika Kronhardt, Roland Benz
Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany
Caroline Stefani, Monica Rolando, Gilles Flatau, Emmanuel Lemichez
Toxines microbiennes dans la relation hôte-pathogènes, C3M, U1065, Inserm, Nice, France
Caroline Stefani, Monica Rolando, Emmanuel Lemichez
UFR Médecine, IFR50, Université de Nice-Sophia Antipolis, Nice, France
Roland Benz
School of Engineering and Science, Jacobs University Bremen, Bremen, Germany

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: CB CS AK MR GF EL RB. Performed the experiments: CB CS AK MR. Analyzed the data: CB CS AK MR EL RB. Contributed reagents/materials/analysis tools: CB CS GF EL RB. Wrote the paper: CB CS MR EL RB.