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Research Article

A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach

  • Qian Mei,

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Xiang Li,

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Yuanguang Meng,

    Affiliation: Department of Gynecologic Oncology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Zhiqiang Wu,

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Mingzhou Guo,

    Affiliation: Department of Cancer Epigenetics, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Yali Zhao,

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Xiaobing Fu mail,

    hanwdrsw69@yahoo.com (WH); fuxiaobing@vip.sina.com (XF)

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Weidong Han mail

    hanwdrsw69@yahoo.com (WH); fuxiaobing@vip.sina.com (XF)

    Affiliation: Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

    X
  • Published: October 05, 2012
  • DOI: 10.1371/journal.pone.0046890

About the Authors

Qian Mei, Xiang Li, Zhiqiang Wu, Yali Zhao, Xiaobing Fu, Weidong Han
Department of Molecular Biology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China
Yuanguang Meng
Department of Gynecologic Oncology, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China
Mingzhou Guo
Department of Cancer Epigenetics, Institute of Basic Medicine, School of Life Sciences, Chinese PLA General Hospital, Beijing, People’s Republic of China

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: QM WH. Performed the experiments: QM XL ZW WH MG YZ. Analyzed the data: QM WH YM XF. Contributed reagents/materials/analysis tools: YM MG. Wrote the paper: QM WH.