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Research Article

Role of the NC-Loop in Catalytic Activity and Stability in Lipase from Fervidobacterium changbaicum

  • Binchun Li equal contributor,

    equal contributor Contributed equally to this work with: Binchun Li, Guangyu Yang

    Affiliations: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China, Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P. R. China

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  • Guangyu Yang equal contributor,

    equal contributor Contributed equally to this work with: Binchun Li, Guangyu Yang

    Affiliation: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China

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  • Lie Wu,

    Affiliations: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China, Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P. R. China

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  • Yan Feng mail

    yfeng2009@sjtu.edu.cn

    Affiliations: State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China, Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P. R. China

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  • Published: October 08, 2012
  • DOI: 10.1371/journal.pone.0046881

About the Authors

Binchun Li, Guangyu Yang, Lie Wu, Yan Feng
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China
Binchun Li, Lie Wu, Yan Feng
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P. R. China

Corresponding Author

Email: yfeng2009@sjtu.edu.cn

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: YF. Performed the experiments: BL GY LW. Analyzed the data: BL GY. Contributed reagents/materials/analysis tools: BL GY. Wrote the paper: BL GY YF.