N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Yaozhong Zou; William I. Weis; Brian K. Kobilka

doi:10.1371/journal.pone.0046039

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Open Access

Peer-reviewed

Research Article

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Yaozhong Zou,

Affiliation Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America
⨯
William I. Weis,

Affiliations Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America, Department of Structural Biology, Stanford University School of Medicine, Stanford, California, United States of America
⨯
Brian K. Kobilka

* E-mail: kobilka@stanford.edu (B.K.K.)

Affiliation Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America
⨯

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

Yaozhong Zou,
William I. Weis,
Brian K. Kobilka

Published: October 4, 2012
https://doi.org/10.1371/journal.pone.0046039

About the Authors

Yaozhong Zou

Affiliation Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America

William I. Weis

Affiliations Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America, Department of Structural Biology, Stanford University School of Medicine, Stanford, California, United States of America

Brian K. Kobilka

* E-mail: kobilka@stanford.edu (B.K.K.)

Affiliation Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: YZ BKK. Performed the experiments: YZ. Analyzed the data: YZ WIW BKK. Contributed reagents/materials/analysis tools: YZ BKK. Wrote the paper: YZ BKK.

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Crystal structure
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G protein coupled receptors
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Crystallization
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Binding analysis
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Crystal lattices
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Point mutation
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Lysozyme
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Protein structure determination
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