Effect of Serum Heat-Inactivation and Dilution on Detection of Anti-WNV Antibodies in Mice by West Nile Virus E-protein Microsphere Immunoassay

Madhuri Namekar; Mukesh Kumar; Maile O'Connell; Vivek R. Nerurkar

doi:10.1371/journal.pone.0045851

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Open Access

Peer-reviewed

Research Article

Effect of Serum Heat-Inactivation and Dilution on Detection of Anti-WNV Antibodies in Mice by West Nile Virus E-protein Microsphere Immunoassay

Madhuri Namekar,

Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
⨯
Mukesh Kumar,

Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
⨯
Maile O'Connell,

Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
⨯
Vivek R. Nerurkar

* E-mail: nerurkar@hawaii.edu

Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
⨯

Effect of Serum Heat-Inactivation and Dilution on Detection of Anti-WNV Antibodies in Mice by West Nile Virus E-protein Microsphere Immunoassay

Madhuri Namekar,
Mukesh Kumar,
Maile O'Connell,
Vivek R. Nerurkar

Published: September 25, 2012
https://doi.org/10.1371/journal.pone.0045851

About the Authors

Madhuri Namekar

Affiliation Department of Tropical Medicine, Medical Microbiology and Pharmacology, Pacific Center for Emerging Infectious Diseases Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America

Mukesh Kumar

Maile O'Connell

Vivek R. Nerurkar

* E-mail: nerurkar@hawaii.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MN VRN. Performed the experiments: MN MK MO. Analyzed the data: MN MK VRN. Contributed reagents/materials/analysis tools: MN VRN. Wrote the paper: MN MK VRN.

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West Nile virus
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Antibodies
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Enzyme-linked immunoassays
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Mouse models
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Complement system
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Respiratory infections
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Antibody production
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Immunoassays
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