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Research Article

Highly Efficient and Specific Genome Editing in Silkworm Using Custom TALENs

  • Sanyuan Ma,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Shengling Zhang,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Feng Wang,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Yong Liu,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Yuanyuan Liu,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Hanfu Xu,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Chun Liu,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Ying Lin,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Ping Zhao,

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Qingyou Xia mail

    xiaqy@swu.edu.cn

    Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

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  • Published: September 18, 2012
  • DOI: 10.1371/journal.pone.0045035
  • Featured in PLOS Collections

About the Authors

Sanyuan Ma, Shengling Zhang, Feng Wang, Yong Liu, Yuanyuan Liu, Hanfu Xu, Chun Liu, Ying Lin, Ping Zhao, Qingyou Xia
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China

Corresponding Author

Email: xiaqy@swu.edu.cn

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SM QX. Performed the experiments: SM SZ Yong Liu Yuanyuan Liu. Analyzed the data: SM SZ QX. Contributed reagents/materials/analysis tools: CL Y. Lin. Wrote the paper: SM QX. Provided suggestions about designing the experiments: HX PZ. Provided protocols about mRNA preparation and performed some of the experiments: FW.