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Research Article

Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing

  • Bradley Michael Zamft equal contributor,

    equal contributor Contributed equally to this work with: Bradley Michael Zamft, Adam H. Marblestone

    Affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America

    X
  • Adam H. Marblestone equal contributor,

    equal contributor Contributed equally to this work with: Bradley Michael Zamft, Adam H. Marblestone

    Affiliations: Biophysics Program, Harvard University, Boston, Massachusetts, United States of America, Wyss Institute, Harvard University, Boston, Massachusetts, United States of America

    X
  • Konrad Kording,

    Affiliations: Northwestern University, Departments of Physical Medicine and Rehabilitation, Physiology, and Applied Mathematics, Chicago, Illinois, United States of America, The Rehabilitation Institute of Chicago, Chicago, Illinois, United States of America

    X
  • Daniel Schmidt,

    Affiliations: Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

    X
  • Daniel Martin-Alarcon,

    Affiliations: Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

    X
  • Keith Tyo,

    Affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America

    X
  • Edward S. Boyden,

    Affiliations: Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

    X
  • George Church mail

    gchurch@genetics.med.harvard.edu

    Affiliations: Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America, Biophysics Program, Harvard University, Boston, Massachusetts, United States of America

    X
  • Published: August 22, 2012
  • DOI: 10.1371/journal.pone.0043876

About the Authors

Bradley Michael Zamft, George Church
Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
Adam H. Marblestone, George Church
Biophysics Program, Harvard University, Boston, Massachusetts, United States of America
Konrad Kording
Northwestern University, Departments of Physical Medicine and Rehabilitation, Physiology, and Applied Mathematics, Chicago, Illinois, United States of America
Daniel Schmidt, Daniel Martin-Alarcon, Edward S. Boyden
Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
Daniel Schmidt, Daniel Martin-Alarcon, Edward S. Boyden
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
Keith Tyo
Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America
Daniel Schmidt, Daniel Martin-Alarcon, Edward S. Boyden
McGovern Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
Konrad Kording
The Rehabilitation Institute of Chicago, Chicago, Illinois, United States of America
Adam H. Marblestone
Wyss Institute, Harvard University, Boston, Massachusetts, United States of America

Corresponding Author

Email: gchurch@genetics.med.harvard.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BMZ AHM KK DS DMA KT ESB GC. Performed the experiments: BMZ AHM DS DMA. Analyzed the data: BMZ AHM KK DS DMA ESB GC. Contributed reagents/materials/analysis tools: BMZ AHM KK DS DMA ESB GC. Wrote the paper: BMZ AHM. Edited the paper: BMZ AHM KK DS DMA KT ESB GC.